Journal: The Journal of Neuroscience
Article Title: The Right Time for a Synapse to Change: Windows and Mechanisms of Multiday Training Trials
doi: 10.1523/JNEUROSCI.1981-25.2026
Figure Lengend Snippet: Phosphorylation of CREB1 and CREB2 after the first stimulus block. A1 , Confocal images of SNs after pCREB1 immunostaining. DAPI was used as a nuclear counterstain. Merged images indicated immunoreactivity to pCREB1 was highest in the nucleus. A2 , Summary data. Different colored circles in each group indicate individual experiments, and the same color in different groups represents one set of experiments. Data in this figure and all other figures are represented as mean ± SEM. ∗ p < 0.05. A3 , No significant (N.S) difference was found in pCREB1 between 5-HT (S) and vehicle (V) groups. B1 , Confocal images of SNs after pCREB2 immunostaining. Immunoreactivity to pCREB2 was also highest in the nucleus. B2 , B3 , Summary data. C , Changes in pCREB1 and pCREB2 at 18 and 24 h. Scale bars, 15 µm.
Article Snippet: The antibody to phosphorylated CREB1 (pCREB1, Genemed Synthesis) utilized the Ser85-phosphorylated CREB1 peptide KKRREILTRRPSYRK epitope ( ).
Techniques: Phospho-proteomics, Blocking Assay, Immunostaining