cpt1b (Proteintech)
Structured Review

Cpt1b, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpt1b/CPT1B-specific+Antibody/pmc12796606-192-32-34
Average 96 stars, based on 132 article reviews
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1) Product Images from "A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer"
Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer
Journal: Bioactive Materials
doi: 10.1016/j.bioactmat.2025.12.039
Figure Legend Snippet: Transcriptomic Analysis Indicates Mg and Al-Mg Alter Gene Expression Patterns in Hepatocellular and Pancreatic Cancer Cells. (A) High-throughput sequencing of PANC-1, PANC-1-Mg, PANC-1-Al-Mg, Huh7, Huh7-Mg, and Huh7-Al-Mg groups; heatmap showing sample correlations. (B) Volcano plots illustrating differential gene expression in Huh7 and PANC-1 cells after Mg or Al-Mg treatment. (C) GO enrichment analysis of differentially expressed genes following Mg or Al-Mg treatment. (D) KEGG pathway enrichment analysis of differentially expressed genes after Mg or Al-Mg treatment. (E) GSEA enrichment analysis of gene expression profiles post Mg or Al-Mg treatment. (F) Western blot analysis of AMPK, p-AMPK and CPT1B expression in PANC-1 cells after Mg or Al-Mg exposure with or without AMPK agonist. (G) PPI network analysis of differentially expressed genes in Mg or Al-Mg-treated cells. (H) Integrated analysis of metabolomic and transcriptomic data by MetaboAnalyst 6.0.
Techniques Used: Gene Expression, Next-Generation Sequencing, Western Blot, Expressing
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Blocking Assay:Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, LG18-1501M − 1H). .. After blocking with 5 % nonfat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies, including AMPK (1:1000, CST, 2532), p-AMPK (1:1000, CST, 2535), Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer. Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, LG18-1501M − 1H). .. After blocking with 5 % nonfat milk for 1 h at room temperature, membranes were incubated overnight at 4 ◦C with primary antibodies, including AMPK (1:1000, CST, 2532), p-AMPK (1:1000, CST, 2535), Incubation:Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, LG18-1501M − 1H). .. After blocking with 5 % nonfat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies, including AMPK (1:1000, CST, 2532), p-AMPK (1:1000, CST, 2535), Article Title: Levocarnitine improves cardiac energy metabolic remodeling in myocarditis mice Article Snippet: .. The PVDF membrane was soaked in 5% skim milk and incubated at room temperature for 2 h. Primary antibodies—GAPDH (60004-1-Ig, Proteintech, Wuhan, China), Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer. Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, LG18-1501M − 1H). .. After blocking with 5 % nonfat milk for 1 h at room temperature, membranes were incubated overnight at 4 ◦C with primary antibodies, including AMPK (1:1000, CST, 2532), p-AMPK (1:1000, CST, 2535), Membrane:Article Title: Levocarnitine improves cardiac energy metabolic remodeling in myocarditis mice Article Snippet: .. The PVDF membrane was soaked in 5% skim milk and incubated at room temperature for 2 h. Primary antibodies—GAPDH (60004-1-Ig, Proteintech, Wuhan, China), Pyrolysis Gas Chromatography:Article Title: Levocarnitine improves cardiac energy metabolic remodeling in myocarditis mice Article Snippet: .. The PVDF membrane was soaked in 5% skim milk and incubated at room temperature for 2 h. Primary antibodies—GAPDH (60004-1-Ig, Proteintech, Wuhan, China), |