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counterstaining with hematoxylin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories counterstaining with hematoxylin
    Counterstaining With Hematoxylin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1582 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/counterstain/VECTOR+Hematoxylin+QS/pmc13101708-56-15-22
    Average 96 stars, based on 1582 article reviews
    counterstaining with hematoxylin - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Construct:

    Article Title: Spermatogonial stem cells and their differentiation roadmap throughout marmoset development
    Article Snippet: In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.. The slides were mounted under glass coverslips with VectaShield Mounting Medium containing 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat H-1200).. Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).

    Article Title: Altered Pulmonary Glucose Transport Is Restored by Metformin Treatment in an Obese Type 2 Diabetic Animal Model
    Article Snippet: Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).. Slides were then treated with Nova Red substrate (Vector Laboratories #SK-4800, Burlingame, CA, USA) for 15 min, followed by a 10 s counterstain with Hematoxylin QS (Vector Laboratories #H-3404-100).. After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.

    Article Title: Repeat associated non-AUG translation as a common mechanism for the polyGln ataxias
    Article Snippet: RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).. Slides were washed in running water (10 mins) and counterstain was performed using Hematoxylin QS (Vector Laboratories) at RT (30 secs for human tissue, 2 mins for mouse tissue).. After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).

    Article Title: Manipulation of eIF3 to modulate repeat associated non-ATG (RAN) translation
    Article Snippet: applied for 30 min at room temperature and detection was performed by exposure to vector nova red substrate kit (Vector Laboratories, Inc., SK4800). .. For counterstain, hematoxylin solution (Vector Laboratories, Inc., H3404) was applied for twenty seconds. .. Slides were dehydrated in graded ethanol and xylene solutions and mounted using Cytoseal 60 (Electron Microscopy Sciences, 18006).

    Article Title: FGFR2 Abrogation Intercepts Pancreatic Ductal Adenocarcinoma Development
    Article Snippet: DAB peroxidase substrate and Vector Blue Alkaline Phosphatase substrate (Vector Laboratories) were used as substrates. .. Hematoxylin (Vector Laboratories) was used as counterstain. .. Cover slides were mounted with Cytoseal 60.

    Article Title: Complement dysregulation in human tauopathies
    Article Snippet: The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.. Chromogenic development was performed using the DAB kit (Vector labs, SK‐4105) using haematoxylin as a counterstain (Vector Labs, H‐3401).. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Article Title: Complement dysregulation in human tauopathies.
    Article Snippet: goat anti-rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK-6100) reagent for 1 h at RT. .. Chromogenic development was performed using the DAB kit (Vector labs, SK-4105) using haematoxylin as a counterstain (Vector Labs, H-3401). .. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Transfection:

    Article Title: Spermatogonial stem cells and their differentiation roadmap throughout marmoset development
    Article Snippet: In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.. The slides were mounted under glass coverslips with VectaShield Mounting Medium containing 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat H-1200).. Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).

    Article Title: Altered Pulmonary Glucose Transport Is Restored by Metformin Treatment in an Obese Type 2 Diabetic Animal Model
    Article Snippet: Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).. Slides were then treated with Nova Red substrate (Vector Laboratories #SK-4800, Burlingame, CA, USA) for 15 min, followed by a 10 s counterstain with Hematoxylin QS (Vector Laboratories #H-3404-100).. After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.

    Article Title: Repeat associated non-AUG translation as a common mechanism for the polyGln ataxias
    Article Snippet: RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).. Slides were washed in running water (10 mins) and counterstain was performed using Hematoxylin QS (Vector Laboratories) at RT (30 secs for human tissue, 2 mins for mouse tissue).. After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).

    Article Title: Manipulation of eIF3 to modulate repeat associated non-ATG (RAN) translation
    Article Snippet: applied for 30 min at room temperature and detection was performed by exposure to vector nova red substrate kit (Vector Laboratories, Inc., SK4800). .. For counterstain, hematoxylin solution (Vector Laboratories, Inc., H3404) was applied for twenty seconds. .. Slides were dehydrated in graded ethanol and xylene solutions and mounted using Cytoseal 60 (Electron Microscopy Sciences, 18006).

    Article Title: FGFR2 Abrogation Intercepts Pancreatic Ductal Adenocarcinoma Development
    Article Snippet: DAB peroxidase substrate and Vector Blue Alkaline Phosphatase substrate (Vector Laboratories) were used as substrates. .. Hematoxylin (Vector Laboratories) was used as counterstain. .. Cover slides were mounted with Cytoseal 60.

    Article Title: Complement dysregulation in human tauopathies
    Article Snippet: The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.. Chromogenic development was performed using the DAB kit (Vector labs, SK‐4105) using haematoxylin as a counterstain (Vector Labs, H‐3401).. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Article Title: Complement dysregulation in human tauopathies.
    Article Snippet: goat anti-rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK-6100) reagent for 1 h at RT. .. Chromogenic development was performed using the DAB kit (Vector labs, SK-4105) using haematoxylin as a counterstain (Vector Labs, H-3401). .. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Plasmid Preparation:

    Article Title: Spermatogonial stem cells and their differentiation roadmap throughout marmoset development
    Article Snippet: In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.. The slides were mounted under glass coverslips with VectaShield Mounting Medium containing 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat H-1200).. Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).

    Article Title: Altered Pulmonary Glucose Transport Is Restored by Metformin Treatment in an Obese Type 2 Diabetic Animal Model
    Article Snippet: Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).. Slides were then treated with Nova Red substrate (Vector Laboratories #SK-4800, Burlingame, CA, USA) for 15 min, followed by a 10 s counterstain with Hematoxylin QS (Vector Laboratories #H-3404-100).. After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.

    Article Title: Repeat associated non-AUG translation as a common mechanism for the polyGln ataxias
    Article Snippet: RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).. Slides were washed in running water (10 mins) and counterstain was performed using Hematoxylin QS (Vector Laboratories) at RT (30 secs for human tissue, 2 mins for mouse tissue).. After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).

    Article Title: Manipulation of eIF3 to modulate repeat associated non-ATG (RAN) translation
    Article Snippet: applied for 30 min at room temperature and detection was performed by exposure to vector nova red substrate kit (Vector Laboratories, Inc., SK4800). .. For counterstain, hematoxylin solution (Vector Laboratories, Inc., H3404) was applied for twenty seconds. .. Slides were dehydrated in graded ethanol and xylene solutions and mounted using Cytoseal 60 (Electron Microscopy Sciences, 18006).

    Article Title: FGFR2 Abrogation Intercepts Pancreatic Ductal Adenocarcinoma Development
    Article Snippet: DAB peroxidase substrate and Vector Blue Alkaline Phosphatase substrate (Vector Laboratories) were used as substrates. .. Hematoxylin (Vector Laboratories) was used as counterstain. .. Cover slides were mounted with Cytoseal 60.

    Article Title: Complement dysregulation in human tauopathies
    Article Snippet: The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.. Chromogenic development was performed using the DAB kit (Vector labs, SK‐4105) using haematoxylin as a counterstain (Vector Labs, H‐3401).. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Article Title: Complement dysregulation in human tauopathies.
    Article Snippet: goat anti-rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK-6100) reagent for 1 h at RT. .. Chromogenic development was performed using the DAB kit (Vector labs, SK-4105) using haematoxylin as a counterstain (Vector Labs, H-3401). .. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Staining:

    Article Title: Spermatogonial stem cells and their differentiation roadmap throughout marmoset development
    Article Snippet: In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.. The slides were mounted under glass coverslips with VectaShield Mounting Medium containing 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat H-1200).. Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).

    Article Title: Altered Pulmonary Glucose Transport Is Restored by Metformin Treatment in an Obese Type 2 Diabetic Animal Model
    Article Snippet: Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).. Slides were then treated with Nova Red substrate (Vector Laboratories #SK-4800, Burlingame, CA, USA) for 15 min, followed by a 10 s counterstain with Hematoxylin QS (Vector Laboratories #H-3404-100).. After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.

    Article Title: Repeat associated non-AUG translation as a common mechanism for the polyGln ataxias
    Article Snippet: RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).. Slides were washed in running water (10 mins) and counterstain was performed using Hematoxylin QS (Vector Laboratories) at RT (30 secs for human tissue, 2 mins for mouse tissue).. After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).

    Article Title: Manipulation of eIF3 to modulate repeat associated non-ATG (RAN) translation
    Article Snippet: applied for 30 min at room temperature and detection was performed by exposure to vector nova red substrate kit (Vector Laboratories, Inc., SK4800). .. For counterstain, hematoxylin solution (Vector Laboratories, Inc., H3404) was applied for twenty seconds. .. Slides were dehydrated in graded ethanol and xylene solutions and mounted using Cytoseal 60 (Electron Microscopy Sciences, 18006).

    Article Title: FGFR2 Abrogation Intercepts Pancreatic Ductal Adenocarcinoma Development
    Article Snippet: DAB peroxidase substrate and Vector Blue Alkaline Phosphatase substrate (Vector Laboratories) were used as substrates. .. Hematoxylin (Vector Laboratories) was used as counterstain. .. Cover slides were mounted with Cytoseal 60.

    Article Title: Complement dysregulation in human tauopathies
    Article Snippet: The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.. Chromogenic development was performed using the DAB kit (Vector labs, SK‐4105) using haematoxylin as a counterstain (Vector Labs, H‐3401).. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Article Title: Complement dysregulation in human tauopathies.
    Article Snippet: goat anti-rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK-6100) reagent for 1 h at RT. .. Chromogenic development was performed using the DAB kit (Vector labs, SK-4105) using haematoxylin as a counterstain (Vector Labs, H-3401). .. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Control:

    Article Title: Spermatogonial stem cells and their differentiation roadmap throughout marmoset development
    Article Snippet: In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.. The slides were mounted under glass coverslips with VectaShield Mounting Medium containing 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat H-1200).. Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).

    Article Title: Altered Pulmonary Glucose Transport Is Restored by Metformin Treatment in an Obese Type 2 Diabetic Animal Model
    Article Snippet: Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).. Slides were then treated with Nova Red substrate (Vector Laboratories #SK-4800, Burlingame, CA, USA) for 15 min, followed by a 10 s counterstain with Hematoxylin QS (Vector Laboratories #H-3404-100).. After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.

    Article Title: Repeat associated non-AUG translation as a common mechanism for the polyGln ataxias
    Article Snippet: RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).. Slides were washed in running water (10 mins) and counterstain was performed using Hematoxylin QS (Vector Laboratories) at RT (30 secs for human tissue, 2 mins for mouse tissue).. After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).

    Article Title: Manipulation of eIF3 to modulate repeat associated non-ATG (RAN) translation
    Article Snippet: applied for 30 min at room temperature and detection was performed by exposure to vector nova red substrate kit (Vector Laboratories, Inc., SK4800). .. For counterstain, hematoxylin solution (Vector Laboratories, Inc., H3404) was applied for twenty seconds. .. Slides were dehydrated in graded ethanol and xylene solutions and mounted using Cytoseal 60 (Electron Microscopy Sciences, 18006).

    Article Title: FGFR2 Abrogation Intercepts Pancreatic Ductal Adenocarcinoma Development
    Article Snippet: DAB peroxidase substrate and Vector Blue Alkaline Phosphatase substrate (Vector Laboratories) were used as substrates. .. Hematoxylin (Vector Laboratories) was used as counterstain. .. Cover slides were mounted with Cytoseal 60.

    Article Title: Complement dysregulation in human tauopathies
    Article Snippet: The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.. Chromogenic development was performed using the DAB kit (Vector labs, SK‐4105) using haematoxylin as a counterstain (Vector Labs, H‐3401).. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Article Title: Complement dysregulation in human tauopathies.
    Article Snippet: goat anti-rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK-6100) reagent for 1 h at RT. .. Chromogenic development was performed using the DAB kit (Vector labs, SK-4105) using haematoxylin as a counterstain (Vector Labs, H-3401). .. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Immunostaining:

    Article Title: Spermatogonial stem cells and their differentiation roadmap throughout marmoset development
    Article Snippet: In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.. The slides were mounted under glass coverslips with VectaShield Mounting Medium containing 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat H-1200).. Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).

    Article Title: Altered Pulmonary Glucose Transport Is Restored by Metformin Treatment in an Obese Type 2 Diabetic Animal Model
    Article Snippet: Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).. Slides were then treated with Nova Red substrate (Vector Laboratories #SK-4800, Burlingame, CA, USA) for 15 min, followed by a 10 s counterstain with Hematoxylin QS (Vector Laboratories #H-3404-100).. After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.

    Article Title: Repeat associated non-AUG translation as a common mechanism for the polyGln ataxias
    Article Snippet: RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).. Slides were washed in running water (10 mins) and counterstain was performed using Hematoxylin QS (Vector Laboratories) at RT (30 secs for human tissue, 2 mins for mouse tissue).. After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).

    Article Title: Manipulation of eIF3 to modulate repeat associated non-ATG (RAN) translation
    Article Snippet: applied for 30 min at room temperature and detection was performed by exposure to vector nova red substrate kit (Vector Laboratories, Inc., SK4800). .. For counterstain, hematoxylin solution (Vector Laboratories, Inc., H3404) was applied for twenty seconds. .. Slides were dehydrated in graded ethanol and xylene solutions and mounted using Cytoseal 60 (Electron Microscopy Sciences, 18006).

    Article Title: FGFR2 Abrogation Intercepts Pancreatic Ductal Adenocarcinoma Development
    Article Snippet: DAB peroxidase substrate and Vector Blue Alkaline Phosphatase substrate (Vector Laboratories) were used as substrates. .. Hematoxylin (Vector Laboratories) was used as counterstain. .. Cover slides were mounted with Cytoseal 60.

    Article Title: Complement dysregulation in human tauopathies
    Article Snippet: The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.. Chromogenic development was performed using the DAB kit (Vector labs, SK‐4105) using haematoxylin as a counterstain (Vector Labs, H‐3401).. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Article Title: Complement dysregulation in human tauopathies.
    Article Snippet: goat anti-rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK-6100) reagent for 1 h at RT. .. Chromogenic development was performed using the DAB kit (Vector labs, SK-4105) using haematoxylin as a counterstain (Vector Labs, H-3401). .. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Transgenic Assay:

    Article Title: Spermatogonial stem cells and their differentiation roadmap throughout marmoset development
    Article Snippet: In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.In this case, the tissues were incubated with the conjugated primary antibodies for 2 hours at room temperature.. The slides were mounted under glass coverslips with VectaShield Mounting Medium containing 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat H-1200).. Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).Immunological signals were captured using an Olympus BX61VS microscope or Evident Slideview VS200 Universal whole slide imaging scanner and evaluated using the software VS-ASW-S6 (Olympus, Hamburg, Germany).

    Article Title: Altered Pulmonary Glucose Transport Is Restored by Metformin Treatment in an Obese Type 2 Diabetic Animal Model
    Article Snippet: Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).Primary antibody incubation was performed overnight at 4 °C using anti-GLUT4 (BioRad #4670-1704, Hercules, CA, USA).. Slides were then treated with Nova Red substrate (Vector Laboratories #SK-4800, Burlingame, CA, USA) for 15 min, followed by a 10 s counterstain with Hematoxylin QS (Vector Laboratories #H-3404-100).. After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.After rinsing with distilled deionized water for 1–2 min, slides were dehydrated and coverslipped.

    Article Title: Repeat associated non-AUG translation as a common mechanism for the polyGln ataxias
    Article Snippet: RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).RAN protein detection was performed by exposure to Vector Red Substrate Kit (Vector Laboratories, Inc.).. Slides were washed in running water (10 mins) and counterstain was performed using Hematoxylin QS (Vector Laboratories) at RT (30 secs for human tissue, 2 mins for mouse tissue).. After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).After washing in water for 5 minutes, slides were dehydrated (ethanol 80%, 95%, 100%, xylene, 20 dips for each step) and mounted using Cytoseal 60 (Electron microscopy sciences).

    Article Title: Manipulation of eIF3 to modulate repeat associated non-ATG (RAN) translation
    Article Snippet: applied for 30 min at room temperature and detection was performed by exposure to vector nova red substrate kit (Vector Laboratories, Inc., SK4800). .. For counterstain, hematoxylin solution (Vector Laboratories, Inc., H3404) was applied for twenty seconds. .. Slides were dehydrated in graded ethanol and xylene solutions and mounted using Cytoseal 60 (Electron Microscopy Sciences, 18006).

    Article Title: FGFR2 Abrogation Intercepts Pancreatic Ductal Adenocarcinoma Development
    Article Snippet: DAB peroxidase substrate and Vector Blue Alkaline Phosphatase substrate (Vector Laboratories) were used as substrates. .. Hematoxylin (Vector Laboratories) was used as counterstain. .. Cover slides were mounted with Cytoseal 60.

    Article Title: Complement dysregulation in human tauopathies
    Article Snippet: The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.The tissue was then incubated in goat anti‐mouse or goat anti‐rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK‐6100) reagent for 1 h at RT.. Chromogenic development was performed using the DAB kit (Vector labs, SK‐4105) using haematoxylin as a counterstain (Vector Labs, H‐3401).. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).

    Article Title: Complement dysregulation in human tauopathies.
    Article Snippet: goat anti-rabbit biotinylated secondary antibody for 1 h at RT followed by Avidin Biotin Complex (ABC, Vector Labs, PK-6100) reagent for 1 h at RT. .. Chromogenic development was performed using the DAB kit (Vector labs, SK-4105) using haematoxylin as a counterstain (Vector Labs, H-3401). .. Tissue sections were dehydrated in industrial methylated spirits and Xylene before mounting in EcoMount (Biocare Medical, EM897L).



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    Image Search Results


    (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Journal: Bioengineering & Translational Medicine

    Article Title: Extracellular matrix microarchitecture modulates cellular behavior and extracellular vesicle phenotypes in biomimetic tendon models

    doi: 10.1002/btm2.70134

    Figure Lengend Snippet: (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Article Snippet: Cellular morphology was assessed via actin staining (Invitrogen, Cat. No. A12379) and nuclear counterstaining with DAPI (Fisher Scientific, Cat. No. H‐1200‐10) ( n = 3).

    Techniques: Staining, Derivative Assay, Control