connective tissue  (David Kopf Instruments)

Journal: Renal Failure

Article Title: Lactate induces renal lipid accumulation and aggravates renal fibrosis by inhibiting the PPARα signaling pathway and fatty acid oxidation

doi: 10.1080/0886022X.2026.2630507

Figure Lengend Snippet: Impaired renal function and renal tissue fibrosis in UUO mice. (A − C) H&E staining and Masson staining in each group ( n = 6). Scale bar = 50 μm. (D and E) BUN and Scr levels in each group ( n = 6). (F − H) Protein expression levels of FN and CTGF in renal tissues ( n = 3). (I and J) mRNA expression levels of FN and CTGF in renal tissues ( n = 6). (K − M) Immunohistochemical staining and quantification for the expression levels of α-SMA and E-cad in renal tissues ( n = 3). Scale bar = 50 μm. ** p < 0.01 vs Sham, *** p < 0.001 vs Sham.

Article Snippet: Primary antibodies against α-smooth muscle actin (α-SMA, AF1032), fibronectin (FN, AF5335), connective tissue growth factor (CTGF, 15f5788), E-cadherin (E-cad, BF0219), peroxisome proliferator-activated receptor α (PPARα, AF5301), peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1α, AF5395), and sterol regulatory element-binding protein 1 (SREBP1, AF6283) were purchased from Affinity Biosciences (Wuhan, China).

Techniques: Staining, Expressing, Immunohistochemical staining

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Aortotomy-induced acute mural thrombosis progresses to saccular aneurysm formation

doi: 10.1016/j.rpth.2026.103365

Figure Lengend Snippet: β-Aminopropionitrile (BAPN) increases the size of mural thrombus at day 7 but not day 21. (A) Sections stained with Verhoeff–Van Gieson (EVG) show mural thrombus in the control and BAPN groups at day 7 and 21. Scale bar, 400 and 200 μm; n = 6. Black-dashed rectangles show the aortotomy site and thrombus; dashed black line shows the thrombus. (B) Merged immunofluorescence of the aortotomy site in the BAPN group at day 7 shows SM22α (green), elastin (ELN; red), and 4′,6-diamidino-2-phenylindole (DAPI; blue); CD31 (green), CTGF (red), and DAPI (blue). White dashed line shows the thrombus. Scale bar, 1 mm or 100 μm; n = 3. (C) Bar graph showing the AT area at day 7 in the control and BAPN groups; ∗ P =.0003, t -test; n = 3. (D) Bar graph showing smooth muscle cell (SMC) layer thickness in the control and rapamycin groups at day 21; ∗ P =.004, t -test; n = 3.

Article Snippet: The sections were then blocked with 2% bovine serum albumin for 1 hour at room temperature, before incubation overnight at 4 °C with the primary antibodies: α-SMA (Abcam; ab5694, IF 1:500); CD31 (R&D; AF3628, IF 1:400); CD41 (Novus; NBP3-15392, IF 1:100); collagen I (Abcam; ab34710, IF 1:50); connective tissue growth factor (Abclonal; A11456, IF 1:100); ELN (Abclonal; A2723, IF 1:100); proliferating cell nuclear antigen (PCNA; Abcam, ab92552, IF 1:50); SM22α (Abcam; ab10135, IF 1:500); vimentin (Abcam, ab45939, IF 1:200); and von Willebrand factor (VWF; Abcam, ab6994, IF 1:200).

Techniques: Staining, Control, Immunofluorescence