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Impaired renal function and renal tissue fibrosis in UUO mice. (A − C) H&E staining and Masson staining in each group ( n = 6). Scale bar = 50 μm. (D and E) BUN and Scr levels in each group ( n = 6). (F − H) Protein expression levels of FN and <t>CTGF</t> in renal tissues ( n = 3). (I and J) mRNA expression levels of FN and CTGF in renal tissues ( n = 6). (K − M) Immunohistochemical staining and quantification for the expression levels of α-SMA and E-cad in renal tissues ( n = 3). Scale bar = 50 μm. ** p < 0.01 vs Sham, *** p < 0.001 vs Sham.
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Impaired renal function and renal tissue fibrosis in UUO mice. (A − C) H&E staining and Masson staining in each group ( n = 6). Scale bar = 50 μm. (D and E) BUN and Scr levels in each group ( n = 6). (F − H) Protein expression levels of FN and <t>CTGF</t> in renal tissues ( n = 3). (I and J) mRNA expression levels of FN and CTGF in renal tissues ( n = 6). (K − M) Immunohistochemical staining and quantification for the expression levels of α-SMA and E-cad in renal tissues ( n = 3). Scale bar = 50 μm. ** p < 0.01 vs Sham, *** p < 0.001 vs Sham.
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Impaired renal function and renal tissue fibrosis in UUO mice. (A − C) H&E staining and Masson staining in each group ( n = 6). Scale bar = 50 μm. (D and E) BUN and Scr levels in each group ( n = 6). (F − H) Protein expression levels of FN and <t>CTGF</t> in renal tissues ( n = 3). (I and J) mRNA expression levels of FN and CTGF in renal tissues ( n = 6). (K − M) Immunohistochemical staining and quantification for the expression levels of α-SMA and E-cad in renal tissues ( n = 3). Scale bar = 50 μm. ** p < 0.01 vs Sham, *** p < 0.001 vs Sham.
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Impaired renal function and renal tissue fibrosis in UUO mice. (A − C) H&E staining and Masson staining in each group ( n = 6). Scale bar = 50 μm. (D and E) BUN and Scr levels in each group ( n = 6). (F − H) Protein expression levels of FN and <t>CTGF</t> in renal tissues ( n = 3). (I and J) mRNA expression levels of FN and CTGF in renal tissues ( n = 6). (K − M) Immunohistochemical staining and quantification for the expression levels of α-SMA and E-cad in renal tissues ( n = 3). Scale bar = 50 μm. ** p < 0.01 vs Sham, *** p < 0.001 vs Sham.
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Impaired renal function and renal tissue fibrosis in UUO mice. (A − C) H&E staining and Masson staining in each group ( n = 6). Scale bar = 50 μm. (D and E) BUN and Scr levels in each group ( n = 6). (F − H) Protein expression levels of FN and <t>CTGF</t> in renal tissues ( n = 3). (I and J) mRNA expression levels of FN and CTGF in renal tissues ( n = 6). (K − M) Immunohistochemical staining and quantification for the expression levels of α-SMA and E-cad in renal tissues ( n = 3). Scale bar = 50 μm. ** p < 0.01 vs Sham, *** p < 0.001 vs Sham.
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Impaired renal function and renal tissue fibrosis in UUO mice. (A − C) H&E staining and Masson staining in each group ( n = 6). Scale bar = 50 μm. (D and E) BUN and Scr levels in each group ( n = 6). (F − H) Protein expression levels of FN and CTGF in renal tissues ( n = 3). (I and J) mRNA expression levels of FN and CTGF in renal tissues ( n = 6). (K − M) Immunohistochemical staining and quantification for the expression levels of α-SMA and E-cad in renal tissues ( n = 3). Scale bar = 50 μm. ** p < 0.01 vs Sham, *** p < 0.001 vs Sham.

Journal: Renal Failure

Article Title: Lactate induces renal lipid accumulation and aggravates renal fibrosis by inhibiting the PPARα signaling pathway and fatty acid oxidation

doi: 10.1080/0886022X.2026.2630507

Figure Lengend Snippet: Impaired renal function and renal tissue fibrosis in UUO mice. (A − C) H&E staining and Masson staining in each group ( n = 6). Scale bar = 50 μm. (D and E) BUN and Scr levels in each group ( n = 6). (F − H) Protein expression levels of FN and CTGF in renal tissues ( n = 3). (I and J) mRNA expression levels of FN and CTGF in renal tissues ( n = 6). (K − M) Immunohistochemical staining and quantification for the expression levels of α-SMA and E-cad in renal tissues ( n = 3). Scale bar = 50 μm. ** p < 0.01 vs Sham, *** p < 0.001 vs Sham.

Article Snippet: Primary antibodies against α-smooth muscle actin (α-SMA, AF1032), fibronectin (FN, AF5335), connective tissue growth factor (CTGF, 15f5788), E-cadherin (E-cad, BF0219), peroxisome proliferator-activated receptor α (PPARα, AF5301), peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1α, AF5395), and sterol regulatory element-binding protein 1 (SREBP1, AF6283) were purchased from Affinity Biosciences (Wuhan, China).

Techniques: Staining, Expressing, Immunohistochemical staining