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avidin biotin peroxidase complex solution  (Vector Laboratories)


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    Structured Review

    Vector Laboratories avidin biotin peroxidase complex solution
    Avidin Biotin Peroxidase Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 16918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Universal)/pm42892892-383-17-25
    Average 96 stars, based on 16918 article reviews
    avidin biotin peroxidase complex solution - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: β-Cyclodextrin-Complexed 1,8-Cineole Attenuates Motor Deficits and Preserves Tyrosine Hydroxylase Immunoreactivity in a Reserpine-Induced Mouse Model of Parkinsonism
    Article Snippet: After primary antibody incubation, the sections were washed and incubated for 2 h with a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, USA) diluted in Triton X-100 solution. .. Sections were subsequently incubated for an additional 2 h with an avidin-biotin-peroxidase complex solution (Vectastain Elite ABC Kit, Vector Laboratories, USA). .. Immunoreactivity was visualized using 3,3′-diaminobenzidine (DAB; Sigma-Aldrich, USA) in the presence of 0.01% H2O2 for 2 min in TH-stained sections and for 1 min in α-Syn-stained sections.

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The next day, the tissues were washed in TBS before being incubated with a goat anti-rabbit biotinylated secondary antibody (1:300, catalog: 111-066-046, Jackson ImmunoResearch) in TBS for 1.5 h at RT, and washed again in TBS. .. The tissues were incubated in an avidin-biotin-complex solution (ABC; 1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories) in TBS for 1 h. The tissues were then washed in TBS and the staining was revealed using incubation with 0.05% of 3,3ʹ diaminobenzidine (DAB) in Tris buffer (TB, 50 mM, pH 8.0) with 0.015% H 2 O 2 for 5 min. ..

    Article Title: Developmental trajectories of somatostatin-positive interneurons in the human auditory cortex from migration to maturation.
    Article Snippet: The sections were again rinsed thrice in PBS and incubated for 2 hours in a secondary antibody solution (Biotinylated anti-rat antibody raised in goat; 1:250 dilution, BA-9400, Vector Laboratories, Burlingame, CA, USA) at RT. .. After this step, sections were rinsed in PBS three times and incubated in a solution containing the avidin-biotin complex (ABC reagent; PK-6100, Vectastain Elite ABC HRP kit, Vector Laboratories, USA) for 2 hours. .. AR TI CL E IN P RE SS ARTICLE IN PRESS This was followed by three rinses in PBS, after which the sections were developed in a solution containing the chromogen (NovaRED substrate kit, peroxidase; SK-4800, Vector Laboratories, USA) according to the manufacturer’s instructions.

    Article Title: Motor sequence analysis as a sensitive biomarker of dopaminergic degeneration in a non-human primate model of parkinsonism
    Article Snippet: .. After three more washes, the sections were incubated in an avidin-biotin enzyme complex solution (ABC kit, PK-4000, Vector, Burlingame, CA) for 1 hour. .. Then, they were rewashed and subjected to the peroxidase detection reaction using DAB (diaminobenzidine – Sigma-Aldrich, Inc) as the chromogen.

    Avidin-Biotin Assay:

    Article Title: β-Cyclodextrin-Complexed 1,8-Cineole Attenuates Motor Deficits and Preserves Tyrosine Hydroxylase Immunoreactivity in a Reserpine-Induced Mouse Model of Parkinsonism
    Article Snippet: After primary antibody incubation, the sections were washed and incubated for 2 h with a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, USA) diluted in Triton X-100 solution. .. Sections were subsequently incubated for an additional 2 h with an avidin-biotin-peroxidase complex solution (Vectastain Elite ABC Kit, Vector Laboratories, USA). .. Immunoreactivity was visualized using 3,3′-diaminobenzidine (DAB; Sigma-Aldrich, USA) in the presence of 0.01% H2O2 for 2 min in TH-stained sections and for 1 min in α-Syn-stained sections.

    Article Title: Precursor Cells in the Parenchyma Act in Concert with Ventricular Neural Progenitors to Facilitate Dramatic Astrocyte Turnover and Recovery Following Natural Neuronal Death
    Article Snippet: Developing solution from the EdU Click-it Kit (Thermo Fisher; Ref No. C10269; biotin azide antibody at 1:800) (component amounts published in online protocol) was then made and applied to slides which were incubated for 2-3 hours at room temperature or overnight at 4C. .. Solution was then thoroughly rinsed and avidin-biotin peroxidase (ABC) complex (Vector Laboratories; SKU PK-6100) was added for signal amplification as per manufacturer’s protocol. ..

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The next day, the tissues were washed in TBS before being incubated with a goat anti-rabbit biotinylated secondary antibody (1:300, catalog: 111-066-046, Jackson ImmunoResearch) in TBS for 1.5 h at RT, and washed again in TBS. .. The tissues were incubated in an avidin-biotin-complex solution (ABC; 1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories) in TBS for 1 h. The tissues were then washed in TBS and the staining was revealed using incubation with 0.05% of 3,3ʹ diaminobenzidine (DAB) in Tris buffer (TB, 50 mM, pH 8.0) with 0.015% H 2 O 2 for 5 min. ..

    Article Title: Developmental trajectories of somatostatin-positive interneurons in the human auditory cortex from migration to maturation.
    Article Snippet: The sections were again rinsed thrice in PBS and incubated for 2 hours in a secondary antibody solution (Biotinylated anti-rat antibody raised in goat; 1:250 dilution, BA-9400, Vector Laboratories, Burlingame, CA, USA) at RT. .. After this step, sections were rinsed in PBS three times and incubated in a solution containing the avidin-biotin complex (ABC reagent; PK-6100, Vectastain Elite ABC HRP kit, Vector Laboratories, USA) for 2 hours. .. AR TI CL E IN P RE SS ARTICLE IN PRESS This was followed by three rinses in PBS, after which the sections were developed in a solution containing the chromogen (NovaRED substrate kit, peroxidase; SK-4800, Vector Laboratories, USA) according to the manufacturer’s instructions.

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa.
    Article Snippet: .. Detection was achieved using a biotinylated secondary antibody diluted in TBS, followed by incubating the slides with avidin-biotin-peroxidase complex solution (ABC, Vectastain Elite ABC Kit; Vector Laboratories, USA) each for 30 minutes at RT and exposure to a 3-amino-9-ethylcarbazole substrate (AEC substrate Kit, Abcam, UK). .. For PAX5 an ImmPRESS Polymer Kit (Vector Laboratories, USA) was used, followed by AEC substrate.

    Article Title: Motor sequence analysis as a sensitive biomarker of dopaminergic degeneration in a non-human primate model of parkinsonism
    Article Snippet: .. After three more washes, the sections were incubated in an avidin-biotin enzyme complex solution (ABC kit, PK-4000, Vector, Burlingame, CA) for 1 hour. .. Then, they were rewashed and subjected to the peroxidase detection reaction using DAB (diaminobenzidine – Sigma-Aldrich, Inc) as the chromogen.

    Article Title: Targeting integrin αvβ3 by chimeric antigen receptor neural stem cell (CAR-NSC) therapy for stroke
    Article Snippet: .. Sections were then treated with the Avidin-Biotin-Complex (ABC) kit solution (Vector Laboratories, #PK-6100) according to the manufacturer’s protocol. ..

    Amplification:

    Article Title: Precursor Cells in the Parenchyma Act in Concert with Ventricular Neural Progenitors to Facilitate Dramatic Astrocyte Turnover and Recovery Following Natural Neuronal Death
    Article Snippet: Developing solution from the EdU Click-it Kit (Thermo Fisher; Ref No. C10269; biotin azide antibody at 1:800) (component amounts published in online protocol) was then made and applied to slides which were incubated for 2-3 hours at room temperature or overnight at 4C. .. Solution was then thoroughly rinsed and avidin-biotin peroxidase (ABC) complex (Vector Laboratories; SKU PK-6100) was added for signal amplification as per manufacturer’s protocol. ..

    Transcranial Magnetic Stimulation:

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The next day, the tissues were washed in TBS before being incubated with a goat anti-rabbit biotinylated secondary antibody (1:300, catalog: 111-066-046, Jackson ImmunoResearch) in TBS for 1.5 h at RT, and washed again in TBS. .. The tissues were incubated in an avidin-biotin-complex solution (ABC; 1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories) in TBS for 1 h. The tissues were then washed in TBS and the staining was revealed using incubation with 0.05% of 3,3ʹ diaminobenzidine (DAB) in Tris buffer (TB, 50 mM, pH 8.0) with 0.015% H 2 O 2 for 5 min. ..

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa.
    Article Snippet: .. Detection was achieved using a biotinylated secondary antibody diluted in TBS, followed by incubating the slides with avidin-biotin-peroxidase complex solution (ABC, Vectastain Elite ABC Kit; Vector Laboratories, USA) each for 30 minutes at RT and exposure to a 3-amino-9-ethylcarbazole substrate (AEC substrate Kit, Abcam, UK). .. For PAX5 an ImmPRESS Polymer Kit (Vector Laboratories, USA) was used, followed by AEC substrate.

    Staining:

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The next day, the tissues were washed in TBS before being incubated with a goat anti-rabbit biotinylated secondary antibody (1:300, catalog: 111-066-046, Jackson ImmunoResearch) in TBS for 1.5 h at RT, and washed again in TBS. .. The tissues were incubated in an avidin-biotin-complex solution (ABC; 1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories) in TBS for 1 h. The tissues were then washed in TBS and the staining was revealed using incubation with 0.05% of 3,3ʹ diaminobenzidine (DAB) in Tris buffer (TB, 50 mM, pH 8.0) with 0.015% H 2 O 2 for 5 min. ..



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    Image Search Results


    UV absorption spectra of PHL solution (A) and zein-PHL mixture solution (B).

    Journal: RSC Advances

    Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

    doi: 10.1039/d5ra08898d

    Figure Lengend Snippet: UV absorption spectra of PHL solution (A) and zein-PHL mixture solution (B).

    Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

    Techniques:

    Effect of PHL on the fluorescence intensity of zein at different temperatures: 298 K (A), 308 K (B), 318 K (C). Stern–Volmer plot of F 0 / F as a function of PHL concentration at different temperatures (D) and plot of log[( F 0 − F )/ F ] as a function of log of PHL concentration (E) in the zein-PHL mixtures at different temperatures. F 0 and F represent the fluorescence intensity in the absence and presence of PHL, respectively.

    Journal: RSC Advances

    Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

    doi: 10.1039/d5ra08898d

    Figure Lengend Snippet: Effect of PHL on the fluorescence intensity of zein at different temperatures: 298 K (A), 308 K (B), 318 K (C). Stern–Volmer plot of F 0 / F as a function of PHL concentration at different temperatures (D) and plot of log[( F 0 − F )/ F ] as a function of log of PHL concentration (E) in the zein-PHL mixtures at different temperatures. F 0 and F represent the fluorescence intensity in the absence and presence of PHL, respectively.

    Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

    Techniques: Fluorescence, Concentration Assay

    CD spectra (A) and secondary structure content (B) of zein-PHL. The PHL concentrations are 0, 10, and 25 µmol L −1 .

    Journal: RSC Advances

    Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

    doi: 10.1039/d5ra08898d

    Figure Lengend Snippet: CD spectra (A) and secondary structure content (B) of zein-PHL. The PHL concentrations are 0, 10, and 25 µmol L −1 .

    Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

    Techniques: Circular Dichroism

    Three-dimensional structure mode of zein-PHL complexes (A); surface structure of PHL in the hydrophobic pocket inside zein (B); binding site of PHL and zein (C).

    Journal: RSC Advances

    Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

    doi: 10.1039/d5ra08898d

    Figure Lengend Snippet: Three-dimensional structure mode of zein-PHL complexes (A); surface structure of PHL in the hydrophobic pocket inside zein (B); binding site of PHL and zein (C).

    Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

    Techniques: Binding Assay