Review



complex solution  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher complex solution
    Complex Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/Tween+20/pm41991526-369-2-31
    Average 99 stars, based on 1 article reviews
    complex solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: PIK3CA mutant cervical cancer is selectively suppressed by PI3Kα inhibition (Alpelisib/BYL-719 and Inavolisib/GDC-0077) and cooperates with HPV directed T cell therapy
    Article Snippet: According to the manufacturer's protocols, whole-cell protein lysates were prepared from cell lines using RIPA lysis buffer supplemented with Complete Protease Inhibitor Cocktail (Santa Cruz, CA, United States). .. In total, 20 - 40 micrograms of whole cell protein were separated in a 4–12% NuPAGE Bis-Tris-gel, transferred to PVDF membrane (Thermo Fisher Scientific, Carlsbad, CA, United States), and immunoblotted with primary antibodies in 0.05% Tween 20-Tris-buffered saline (TBST) containing 5% BSA at 4°C with shaking overnight. .. The primary antibodies used in this study were: rabbit monoclonal anti-PD-L1, (Cell Signaling Technology Cat# 13684, RRID: AB_2687655), YAP1, (Cell Signaling Technology Cat# 14074, RRID: AB_2650491), EGFR (Cell Signaling Technology Cat# 4267, RRID: AB_2800085), CTGF (Cell Signaling Technology Cat# 86641, RRID: AB_2800085), Integrin α6 (Cell Signaling Technology Cat# 3750, RRID: AB_2249263), IRF1 (Cell Signaling Technology Cat# 8478, RRID: AB_10949108) at a dilution from1:250 to 1:500; mouse monoclonal anti-HPV16 E7 at 1:100 (Santa Cruz Cat# sc-51951, RRID: AB_629662), and goat monoclonal anti-p16 at 1:500 (R&D system Cat# AF5779, RRID: AB_1964666). β-actin, used as an internal control, was detected by rabbit anti-β-actin antibody (Cell Signaling Technology Cat# 4967, RRID: AB_330288).

    Saline:

    Article Title: PIK3CA mutant cervical cancer is selectively suppressed by PI3Kα inhibition (Alpelisib/BYL-719 and Inavolisib/GDC-0077) and cooperates with HPV directed T cell therapy
    Article Snippet: According to the manufacturer's protocols, whole-cell protein lysates were prepared from cell lines using RIPA lysis buffer supplemented with Complete Protease Inhibitor Cocktail (Santa Cruz, CA, United States). .. In total, 20 - 40 micrograms of whole cell protein were separated in a 4–12% NuPAGE Bis-Tris-gel, transferred to PVDF membrane (Thermo Fisher Scientific, Carlsbad, CA, United States), and immunoblotted with primary antibodies in 0.05% Tween 20-Tris-buffered saline (TBST) containing 5% BSA at 4°C with shaking overnight. .. The primary antibodies used in this study were: rabbit monoclonal anti-PD-L1, (Cell Signaling Technology Cat# 13684, RRID: AB_2687655), YAP1, (Cell Signaling Technology Cat# 14074, RRID: AB_2650491), EGFR (Cell Signaling Technology Cat# 4267, RRID: AB_2800085), CTGF (Cell Signaling Technology Cat# 86641, RRID: AB_2800085), Integrin α6 (Cell Signaling Technology Cat# 3750, RRID: AB_2249263), IRF1 (Cell Signaling Technology Cat# 8478, RRID: AB_10949108) at a dilution from1:250 to 1:500; mouse monoclonal anti-HPV16 E7 at 1:100 (Santa Cruz Cat# sc-51951, RRID: AB_629662), and goat monoclonal anti-p16 at 1:500 (R&D system Cat# AF5779, RRID: AB_1964666). β-actin, used as an internal control, was detected by rabbit anti-β-actin antibody (Cell Signaling Technology Cat# 4967, RRID: AB_330288).

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to separate lysates with 4-12% Bis-Tris gels (Thermo Scientific) before transfer to PVDF membranes using an iBlot3 (Invitrogen). .. Blocking with 5% BSA in Tris-buffered saline with Tween 20 (TBST) for 1 h at room temperature was followed by overnight incubation with primary antibodies diluted in SuperBlock blocking buffers (Thermo Fisher Scientific) at 4 °C. ..

    Lysis:

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. Cells were resuspended in Cell Lysis Buffer [25 mM KCl (Sigma–Aldrich, 27810.295), 5 mM TRIS (Sigma–Aldrich, 0497–5KG) pH 8.3, 1.25 mM MgCl 2 (VWR BDH7899-1), 0.225% IGEPAL (Sigma–Aldrich, I8896-50 ML), and 0.225% Tween20 (VWR)] supplemented with 0.4 μg/μl Proteinase K (Thermo Fisher Scientific, EO0492), vortexed, and incubated sequentially at 65°C for 6 min and 95°C for 2 min. Lysates were cooled down, and 1–2 μl were used directly for polymerase chain reaction (PCR). .. For applications requiring high-purity gDNA, including Sanger sequencing, gDNA was isolated using the NZY Tissue gDNA Isolation Kit (NZYTech, MB13503) according to the manufacturer’s instructions.

    Incubation:

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. Cells were resuspended in Cell Lysis Buffer [25 mM KCl (Sigma–Aldrich, 27810.295), 5 mM TRIS (Sigma–Aldrich, 0497–5KG) pH 8.3, 1.25 mM MgCl 2 (VWR BDH7899-1), 0.225% IGEPAL (Sigma–Aldrich, I8896-50 ML), and 0.225% Tween20 (VWR)] supplemented with 0.4 μg/μl Proteinase K (Thermo Fisher Scientific, EO0492), vortexed, and incubated sequentially at 65°C for 6 min and 95°C for 2 min. Lysates were cooled down, and 1–2 μl were used directly for polymerase chain reaction (PCR). .. For applications requiring high-purity gDNA, including Sanger sequencing, gDNA was isolated using the NZY Tissue gDNA Isolation Kit (NZYTech, MB13503) according to the manufacturer’s instructions.

    Article Title: Distinct hyaluronan content and hierarchical organization of collagen-elastic fiber assemblies in subcutaneous fascia
    Article Snippet: .. Samples were incubated with elastin monoclonal antibody BA-4 (MA1-27129, Thermo Fisher, Waltham, MA, USA; dilution 1: 100 in T-PBS containing 0.1% BSA) for 1 h at room temperature, washed three times for 5 min each with T-PBS (0.1% Tween-20 in PBS), and then reacted with Alexa Fluor 594–conjugated goat anti-mouse IgG (A11032; Thermo Fisher; dilution 1:1000 in T-PBS containing 0.1% BSA) for 1 h at room temperature [ ]. .. According to the manufacturer's protocol, 20 μM CHP (FLU60; 3Helix/Funakoshi) dissolved in PBS was heated at 80 °C for 5 min, rapidly cooled in an ice bath, and centrifuged; the supernatant was then used without further dilution [ , , ].

    Article Title: Silencing of SIVA-1 promotes cisplatin resistance in gastric cancer via the Bcl-2/BAX-mediated mitochondria-dependent apoptosis pathway
    Article Snippet: .. Subsequently, Goat Anti-Rabbit IgG H&L (HRP) (cat. no. ab6721; Abcam) was added and incubated for 1 h at room temperature in the dark, and washed three times with 0.1% TBS Tween-20 Buffer (cat. no. 28360; Thermo Fisher Scientific, Inc.). .. A gel imager (iBright CL750; Invitrogen; Thermo Fisher Scientific, Inc.) was used to scan the protein blotting membrane, and the gray scale values of the bands were analyzed and recorded using ImageJ software (bundled with 64-bit Java 8; National Institutes of Health).

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to separate lysates with 4-12% Bis-Tris gels (Thermo Scientific) before transfer to PVDF membranes using an iBlot3 (Invitrogen). .. Blocking with 5% BSA in Tris-buffered saline with Tween 20 (TBST) for 1 h at room temperature was followed by overnight incubation with primary antibodies diluted in SuperBlock blocking buffers (Thermo Fisher Scientific) at 4 °C. ..

    Polymerase Chain Reaction:

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. Cells were resuspended in Cell Lysis Buffer [25 mM KCl (Sigma–Aldrich, 27810.295), 5 mM TRIS (Sigma–Aldrich, 0497–5KG) pH 8.3, 1.25 mM MgCl 2 (VWR BDH7899-1), 0.225% IGEPAL (Sigma–Aldrich, I8896-50 ML), and 0.225% Tween20 (VWR)] supplemented with 0.4 μg/μl Proteinase K (Thermo Fisher Scientific, EO0492), vortexed, and incubated sequentially at 65°C for 6 min and 95°C for 2 min. Lysates were cooled down, and 1–2 μl were used directly for polymerase chain reaction (PCR). .. For applications requiring high-purity gDNA, including Sanger sequencing, gDNA was isolated using the NZY Tissue gDNA Isolation Kit (NZYTech, MB13503) according to the manufacturer’s instructions.

    Blocking Assay:

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to separate lysates with 4-12% Bis-Tris gels (Thermo Scientific) before transfer to PVDF membranes using an iBlot3 (Invitrogen). .. Blocking with 5% BSA in Tris-buffered saline with Tween 20 (TBST) for 1 h at room temperature was followed by overnight incubation with primary antibodies diluted in SuperBlock blocking buffers (Thermo Fisher Scientific) at 4 °C. ..



    Similar Products

    99
    Vector Laboratories avidin biotin complex solution
    Avidin Biotin Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc12996694-112-6-15
    Average 99 stars, based on 1 article reviews
    avidin biotin complex solution - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    96
    JASCO Inc zein phl complex solutions
    UV absorption spectra of PHL solution (A) <t>and</t> <t>zein-PHL</t> mixture solution (B).
    Zein Phl Complex Solutions, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/J-1500/pmc12879988-66-5-15
    Average 96 stars, based on 1 article reviews
    zein phl complex solutions - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    99
    Thermo Fisher complex solution
    UV absorption spectra of PHL solution (A) <t>and</t> <t>zein-PHL</t> mixture solution (B).
    Complex Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/Tween+20/pm41991526-369-2-31
    Average 99 stars, based on 1 article reviews
    complex solution - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories avidin biotin complex abc kit solution
    UV absorption spectra of PHL solution (A) <t>and</t> <t>zein-PHL</t> mixture solution (B).
    Avidin Biotin Complex Abc Kit Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/bio_rxiv__64898__2026__04__11__717950-265-6-10
    Average 99 stars, based on 1 article reviews
    avidin biotin complex abc kit solution - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories avidin biotin complex abc solution
    UV absorption spectra of PHL solution (A) <t>and</t> <t>zein-PHL</t> mixture solution (B).
    Avidin Biotin Complex Abc Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/bio_rxiv__64898__2026__04__06__716383-168-12-16
    Average 99 stars, based on 1 article reviews
    avidin biotin complex abc solution - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher copper ii chloride
    UV absorption spectra of PHL solution (A) <t>and</t> <t>zein-PHL</t> mixture solution (B).
    Copper Ii Chloride, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/Copper(II)-ethylenediamine+complex%2C+1M+solution+in+water/pmc13003922-92-0-9
    Average 94 stars, based on 1 article reviews
    copper ii chloride - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    86
    Nichirei Corporation streptavidin peroxidase complex solution
    UV absorption spectra of PHL solution (A) <t>and</t> <t>zein-PHL</t> mixture solution (B).
    Streptavidin Peroxidase Complex Solution, supplied by Nichirei Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/biotin+complex+peroxidase+streptoavidin/pm41702140-55-6-12
    Average 86 stars, based on 1 article reviews
    streptavidin peroxidase complex solution - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    94
    Thermo Fisher copper ii ethylenediamine complex ced
    UV absorption spectra of PHL solution (A) <t>and</t> <t>zein-PHL</t> mixture solution (B).
    Copper Ii Ethylenediamine Complex Ced, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/Copper(II)-ethylenediamine+complex%2C+1M+solution+in+water/pm41577279-71-0-15
    Average 94 stars, based on 1 article reviews
    copper ii ethylenediamine complex ced - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    99
    Vector Laboratories incubatedwith avidin biotinylated enzyme complex abc solution
    UV absorption spectra of PHL solution (A) <t>and</t> <t>zein-PHL</t> mixture solution (B).
    Incubatedwith Avidin Biotinylated Enzyme Complex Abc Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complex+solution/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pm41540212-404-8-16
    Average 99 stars, based on 1 article reviews
    incubatedwith avidin biotinylated enzyme complex abc solution - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    UV absorption spectra of PHL solution (A) and zein-PHL mixture solution (B).

    Journal: RSC Advances

    Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

    doi: 10.1039/d5ra08898d

    Figure Lengend Snippet: UV absorption spectra of PHL solution (A) and zein-PHL mixture solution (B).

    Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

    Techniques:

    Effect of PHL on the fluorescence intensity of zein at different temperatures: 298 K (A), 308 K (B), 318 K (C). Stern–Volmer plot of F 0 / F as a function of PHL concentration at different temperatures (D) and plot of log[( F 0 − F )/ F ] as a function of log of PHL concentration (E) in the zein-PHL mixtures at different temperatures. F 0 and F represent the fluorescence intensity in the absence and presence of PHL, respectively.

    Journal: RSC Advances

    Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

    doi: 10.1039/d5ra08898d

    Figure Lengend Snippet: Effect of PHL on the fluorescence intensity of zein at different temperatures: 298 K (A), 308 K (B), 318 K (C). Stern–Volmer plot of F 0 / F as a function of PHL concentration at different temperatures (D) and plot of log[( F 0 − F )/ F ] as a function of log of PHL concentration (E) in the zein-PHL mixtures at different temperatures. F 0 and F represent the fluorescence intensity in the absence and presence of PHL, respectively.

    Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

    Techniques: Fluorescence, Concentration Assay

    CD spectra (A) and secondary structure content (B) of zein-PHL. The PHL concentrations are 0, 10, and 25 µmol L −1 .

    Journal: RSC Advances

    Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

    doi: 10.1039/d5ra08898d

    Figure Lengend Snippet: CD spectra (A) and secondary structure content (B) of zein-PHL. The PHL concentrations are 0, 10, and 25 µmol L −1 .

    Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

    Techniques: Circular Dichroism

    Three-dimensional structure mode of zein-PHL complexes (A); surface structure of PHL in the hydrophobic pocket inside zein (B); binding site of PHL and zein (C).

    Journal: RSC Advances

    Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

    doi: 10.1039/d5ra08898d

    Figure Lengend Snippet: Three-dimensional structure mode of zein-PHL complexes (A); surface structure of PHL in the hydrophobic pocket inside zein (B); binding site of PHL and zein (C).

    Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

    Techniques: Binding Assay