Journal: Journal of Advanced Research
Article Title: Metformin attenuates colitis via blocking STAT3 acetylation by reducing acetyl-CoA production
doi: 10.1016/j.jare.2025.03.058
Figure Lengend Snippet: Metformin attenuates colitis by suppressing the STAT3 signaling pathway. (A) Experimental flow chart: STAT3 fl/fl and STAT3 ΔIEC mice (n = 6) were administered metformin (200 mg/kg) or PBS intraperitoneally, followed by continuous delivery of 3 % DSS in their drinking water for 7 days. (B-C) Changes in body weight and DAI of mice were monitored from day 1. (D-E) A comparison of colon length and morphology in mice. (F) Representative images of H&E morphology, along with IHC analysis of AB-PAS and MUC-2. (G) Histological scores of colon tissue from mice in various treatment groups. (H-I) Representative immunofluorescence images of ZO-1 and TUNEL staining. (J) Scanning electron microscopy images of mouse colonic tissue sections. (K) WB analysis of colonic protein expression in mice, including STAT3, p-STAT3 Y705 , E-cadherin, Occludin, Bcl-2, and Bax. (L) IHC analysis of p-STAT3 Y705 expression in mouse colonic tissue. (M) WB analysis of protein expression in NCM460 cells. The cells were transfected with siSTAT3 or siNC using Lipofectamine 3000, followed by treatment with or without metformin (200 μM for 24 h) and stimulation with LPS (1 μg/ml for 12 h). (N-P) Representative IF images showing ZO-1, p-STAT3 Y705 , and STAT3 expression in cells. (Q) WB analysis of relevant protein levels in NCM460 cells. The cells were cocultured with LPS (1 μg/mL for 12 h) and transfected with a STAT3 overexpression plasmid (STAT3 WT ) or negative control (NC), with or without metformin pretreatment (200 μM for 24 h). (R) WB assessment of relevant protein levels in NCM460 cells. The cells were treated with DMSO or Colivelin TFA (25 μg/ml for 13 h, Med Chem Express, #HY-P1061A) following treatment with LPS (1 μg/ml for 12 h) and metformin (200 μM for 24 h).
Article Snippet: The cells were treated with DMSO or Colivelin TFA (25 μg/ml for 13 h, Med Chem Express, #HY-P1061A) following treatment with LPS (1 μg/ml for 12 h) and metformin (200 μM for 24 h).
Techniques: Comparison, Immunofluorescence, TUNEL Assay, Staining, Electron Microscopy, Expressing, Paraffin-embedded Immunohistochemistry, Transfection, Over Expression, Plasmid Preparation, Negative Control
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