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mouse colorectal cancer cell lines cmt93  (ATCC)


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    ATCC mouse colorectal cancer cell lines cmt93
    Mouse Colorectal Cancer Cell Lines Cmt93, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse colorectal cancer cell lines cmt93/product/ATCC
    Average 95 stars, based on 281 article reviews
    mouse colorectal cancer cell lines cmt93 - by Bioz Stars, 2026-05
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    Equal inoculums of CM TS1 , M7, or M8 were used to infect CMT93, C57, and McCoy cells, and IFU were counted at 24 hpi. The ratio of IFUs that the strains formed in (A) CMT93 versus McCoy cells and in (B) C57 versus McCoy cells were compared. Significance was determined by ordinary one-way ANOVA with Dunnett’s multiple comparisons posttest. Error bars represent standard deviation. **, P < 0.01; ****, P < 0.0001.

    Journal: PLOS One

    Article Title: Isolation and characterization of a Chlamydia muridarum tc0237 mutant from a genetic screen that is attenuated in epithelial cells

    doi: 10.1371/journal.pone.0329637

    Figure Lengend Snippet: Equal inoculums of CM TS1 , M7, or M8 were used to infect CMT93, C57, and McCoy cells, and IFU were counted at 24 hpi. The ratio of IFUs that the strains formed in (A) CMT93 versus McCoy cells and in (B) C57 versus McCoy cells were compared. Significance was determined by ordinary one-way ANOVA with Dunnett’s multiple comparisons posttest. Error bars represent standard deviation. **, P < 0.01; ****, P < 0.0001.

    Article Snippet: Murine fibroblast (McCoy cells) and murine rectal carcinoma (CMT93) cell lines were obtained from the American Type Culture Collection (ATCC) and were maintained in high glucose Dulbecco’s Modified Eagle medium (DMEM; Cytiva Hyclone) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals) non-essential amino acids, and HEPES (DMEM-10).

    Techniques: Standard Deviation

    Equal inocula of CM TS1 , M7, or M8 were used to infect (A) CMT93 and (B) C57 cells + / − IFNγ, and IFU were counted at 24 hpi. IFUs that the strains formed in the cell lines + / − IFNγ were compared. Significance was determined by ordinary one-way ANOVA with Dunnett’s multiple comparison posttest. Error bars represent standard deviation. ***, P < 0.001; ****, P < 0.0001.

    Journal: PLOS One

    Article Title: Isolation and characterization of a Chlamydia muridarum tc0237 mutant from a genetic screen that is attenuated in epithelial cells

    doi: 10.1371/journal.pone.0329637

    Figure Lengend Snippet: Equal inocula of CM TS1 , M7, or M8 were used to infect (A) CMT93 and (B) C57 cells + / − IFNγ, and IFU were counted at 24 hpi. IFUs that the strains formed in the cell lines + / − IFNγ were compared. Significance was determined by ordinary one-way ANOVA with Dunnett’s multiple comparison posttest. Error bars represent standard deviation. ***, P < 0.001; ****, P < 0.0001.

    Article Snippet: Murine fibroblast (McCoy cells) and murine rectal carcinoma (CMT93) cell lines were obtained from the American Type Culture Collection (ATCC) and were maintained in high glucose Dulbecco’s Modified Eagle medium (DMEM; Cytiva Hyclone) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals) non-essential amino acids, and HEPES (DMEM-10).

    Techniques: Comparison, Standard Deviation

    M8, various recombinants, CM TS1 , or CM tsp were used to infect McCoy, ( A ) CMT93, and ( B ) C57 cells at MOIs of 1.0, and inclusions were counted at 24 hpi. Ratios of IFUs that the strains formed in CMT93 or C57 cells divided by the IFUs formed in McCoy cells are shown on the y-axis. Significance was determined by ordinary one-way ANOVA with Tukey’s multiple comparison test. Shared letters indicate groups with p-values greater than 0.05. Groups that do not share a letter have p-values less than 0.05. Error bars represent standard deviation.

    Journal: PLOS One

    Article Title: Isolation and characterization of a Chlamydia muridarum tc0237 mutant from a genetic screen that is attenuated in epithelial cells

    doi: 10.1371/journal.pone.0329637

    Figure Lengend Snippet: M8, various recombinants, CM TS1 , or CM tsp were used to infect McCoy, ( A ) CMT93, and ( B ) C57 cells at MOIs of 1.0, and inclusions were counted at 24 hpi. Ratios of IFUs that the strains formed in CMT93 or C57 cells divided by the IFUs formed in McCoy cells are shown on the y-axis. Significance was determined by ordinary one-way ANOVA with Tukey’s multiple comparison test. Shared letters indicate groups with p-values greater than 0.05. Groups that do not share a letter have p-values less than 0.05. Error bars represent standard deviation.

    Article Snippet: Murine fibroblast (McCoy cells) and murine rectal carcinoma (CMT93) cell lines were obtained from the American Type Culture Collection (ATCC) and were maintained in high glucose Dulbecco’s Modified Eagle medium (DMEM; Cytiva Hyclone) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals) non-essential amino acids, and HEPES (DMEM-10).

    Techniques: Comparison, Standard Deviation

    McCoy cells were infected with M8R4-pTC0237-FLAG at an MOI of 1.0 in +/ − aTc and the infected cells were lysed 24 hours later. The lysates, M8, M8R4 237 mut , M8R24 237 wt , CM TS1 , and CM tsp were used to infect fresh McCoy, (A) CMT93, or (B) C57 cells, and IFUs were determined at 24 hpi. (C) McCoy, CMT93, or C57 cells were infected with M8R4-pTC0237-FLAG at MOIs of 0.1, fresh medium + / − aTc was added at 2 hpi, and IFUs were determined at 24 hpi. The number of IFUs formed in each condition is on the y-axis and each dot represents the result of a technical replicate. Significance was determined by ( A-B ) ordinary one-way ANOVA with Tukey’s multiple comparison test or ( C ) unpaired t-test. Shared letters indicate groups with p-values greater than 0.05. Groups that do not share a letter have p-values less than 0.05. Error bars represent standard deviation. ns = not significant.

    Journal: PLOS One

    Article Title: Isolation and characterization of a Chlamydia muridarum tc0237 mutant from a genetic screen that is attenuated in epithelial cells

    doi: 10.1371/journal.pone.0329637

    Figure Lengend Snippet: McCoy cells were infected with M8R4-pTC0237-FLAG at an MOI of 1.0 in +/ − aTc and the infected cells were lysed 24 hours later. The lysates, M8, M8R4 237 mut , M8R24 237 wt , CM TS1 , and CM tsp were used to infect fresh McCoy, (A) CMT93, or (B) C57 cells, and IFUs were determined at 24 hpi. (C) McCoy, CMT93, or C57 cells were infected with M8R4-pTC0237-FLAG at MOIs of 0.1, fresh medium + / − aTc was added at 2 hpi, and IFUs were determined at 24 hpi. The number of IFUs formed in each condition is on the y-axis and each dot represents the result of a technical replicate. Significance was determined by ( A-B ) ordinary one-way ANOVA with Tukey’s multiple comparison test or ( C ) unpaired t-test. Shared letters indicate groups with p-values greater than 0.05. Groups that do not share a letter have p-values less than 0.05. Error bars represent standard deviation. ns = not significant.

    Article Snippet: Murine fibroblast (McCoy cells) and murine rectal carcinoma (CMT93) cell lines were obtained from the American Type Culture Collection (ATCC) and were maintained in high glucose Dulbecco’s Modified Eagle medium (DMEM; Cytiva Hyclone) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals) non-essential amino acids, and HEPES (DMEM-10).

    Techniques: Infection, Comparison, Standard Deviation

    McCoy (black circles) and CMT93 (white squares) cells were infected with Cmu wt , M8, M8R4 237mut , M8R24 237wt , and M8R4-pTC237-FLAG + / − aTc. The number of IFUs produced by these infections was compared to the input inoculum of the primary infection to determine the relative burst size (y-axis) at various hpi (x-axis). Relative burst sizes of infections in McCoy and CMT93 cells were compared by ordinary two-way ANOVA with Bonferroni correction. Graph shows the results from two independent trials, in technical triplicates. Error bars represent standard deviation. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

    Journal: PLOS One

    Article Title: Isolation and characterization of a Chlamydia muridarum tc0237 mutant from a genetic screen that is attenuated in epithelial cells

    doi: 10.1371/journal.pone.0329637

    Figure Lengend Snippet: McCoy (black circles) and CMT93 (white squares) cells were infected with Cmu wt , M8, M8R4 237mut , M8R24 237wt , and M8R4-pTC237-FLAG + / − aTc. The number of IFUs produced by these infections was compared to the input inoculum of the primary infection to determine the relative burst size (y-axis) at various hpi (x-axis). Relative burst sizes of infections in McCoy and CMT93 cells were compared by ordinary two-way ANOVA with Bonferroni correction. Graph shows the results from two independent trials, in technical triplicates. Error bars represent standard deviation. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

    Article Snippet: Murine fibroblast (McCoy cells) and murine rectal carcinoma (CMT93) cell lines were obtained from the American Type Culture Collection (ATCC) and were maintained in high glucose Dulbecco’s Modified Eagle medium (DMEM; Cytiva Hyclone) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals) non-essential amino acids, and HEPES (DMEM-10).

    Techniques: Infection, Produced, Standard Deviation

    McCoy (black circles) and CMT93 (white squares) cells were infected with Cmu wt , M8, M8R4 237mut , M8R24 237wt , and M8R4-pTC237-FLAG + / − aTc without centrifugation. The number of IFUs produced was compared to the input IFUs of the primary infection to determine the relative burst size (y-axis) at various hpi (x-axis). Relative burst sizes of infections in McCoy and CMT93 cells were compared by ordinary two-way ANOVA with Bonferroni correction. The graphs show the results from two independent experiment performed in technical triplicates. Error bars indicate standard deviation. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

    Journal: PLOS One

    Article Title: Isolation and characterization of a Chlamydia muridarum tc0237 mutant from a genetic screen that is attenuated in epithelial cells

    doi: 10.1371/journal.pone.0329637

    Figure Lengend Snippet: McCoy (black circles) and CMT93 (white squares) cells were infected with Cmu wt , M8, M8R4 237mut , M8R24 237wt , and M8R4-pTC237-FLAG + / − aTc without centrifugation. The number of IFUs produced was compared to the input IFUs of the primary infection to determine the relative burst size (y-axis) at various hpi (x-axis). Relative burst sizes of infections in McCoy and CMT93 cells were compared by ordinary two-way ANOVA with Bonferroni correction. The graphs show the results from two independent experiment performed in technical triplicates. Error bars indicate standard deviation. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

    Article Snippet: Murine fibroblast (McCoy cells) and murine rectal carcinoma (CMT93) cell lines were obtained from the American Type Culture Collection (ATCC) and were maintained in high glucose Dulbecco’s Modified Eagle medium (DMEM; Cytiva Hyclone) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals) non-essential amino acids, and HEPES (DMEM-10).

    Techniques: Infection, Centrifugation, Produced, Standard Deviation