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cmf2hc staining solutions  (MedChemExpress)


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    MedChemExpress cmf2hc staining solutions
    Cmf2hc Staining Solutions, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 967 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cmf2hc/pmc12975322-115-19-23?v=MedChemExpress
    Average 99 stars, based on 967 article reviews
    cmf2hc staining solutions - by Bioz Stars, 2026-07
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    EFNA1 alleviates excessive misfolded protein accumulation in oocytes during IVM. ( A ) GSEA analysis of differential expression genes related to oxidative phosphorylation, glutathione metabolism, and reactive oxygen species in in vitro matured oocytes compared to in vivo . ( B ) Effect of 10 ng/mL EFNA1 on ROS and GSH levels in matured sheep oocytes. Intracellular ROS and GSH levels were measured using CM-H2DCFDA and CellTracker™ Blue <t>CMF2HC</t> fluorescent probes, respectively (Scale bar = 200 µm). ( C ) Effect of 10 ng/mL EFNA1 on protein aggregates content in matured sheep oocytes. The protein aggregates content was determined by proteoStat aggresome detection reagent labeling (Scale bar = 50 µm). ( D ) Effect of 10 ng/mL EFNA1 on proteasome activity in matured sheep oocytes. The proteasome activity was evaluated by Me4BodipyFL (Scale bar = 50 µm). ( E , F ) Immunofluorescence detection of ER stress-associated protein (GRP78, ATF6) in matured sheep oocytes cultured with or without EFNA1 (Scale bar = 50 µm). ( G ) Relative expression levels of ER stress-associated genes (GRP78, PERK, EIF2A, ATF4, ATF6, IRE1, XBP1) in matured sheep oocytes cultured with or without EFNA1 as analyzed by RT-PCR. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.
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    EFNA1 alleviates excessive misfolded protein accumulation in oocytes during IVM. ( A ) GSEA analysis of differential expression genes related to oxidative phosphorylation, glutathione metabolism, and reactive oxygen species in in vitro matured oocytes compared to in vivo . ( B ) Effect of 10 ng/mL EFNA1 on ROS and GSH levels in matured sheep oocytes. Intracellular ROS and GSH levels were measured using CM-H2DCFDA and CellTracker™ Blue CMF2HC fluorescent probes, respectively (Scale bar = 200 µm). ( C ) Effect of 10 ng/mL EFNA1 on protein aggregates content in matured sheep oocytes. The protein aggregates content was determined by proteoStat aggresome detection reagent labeling (Scale bar = 50 µm). ( D ) Effect of 10 ng/mL EFNA1 on proteasome activity in matured sheep oocytes. The proteasome activity was evaluated by Me4BodipyFL (Scale bar = 50 µm). ( E , F ) Immunofluorescence detection of ER stress-associated protein (GRP78, ATF6) in matured sheep oocytes cultured with or without EFNA1 (Scale bar = 50 µm). ( G ) Relative expression levels of ER stress-associated genes (GRP78, PERK, EIF2A, ATF4, ATF6, IRE1, XBP1) in matured sheep oocytes cultured with or without EFNA1 as analyzed by RT-PCR. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Antioxidants

    Article Title: Cross-Species Insights into In Vitro Maturation Defects of the Oocyte and Identification of Crucial Regulators for Sheep Oocyte Maturation

    doi: 10.3390/antiox14121499

    Figure Lengend Snippet: EFNA1 alleviates excessive misfolded protein accumulation in oocytes during IVM. ( A ) GSEA analysis of differential expression genes related to oxidative phosphorylation, glutathione metabolism, and reactive oxygen species in in vitro matured oocytes compared to in vivo . ( B ) Effect of 10 ng/mL EFNA1 on ROS and GSH levels in matured sheep oocytes. Intracellular ROS and GSH levels were measured using CM-H2DCFDA and CellTracker™ Blue CMF2HC fluorescent probes, respectively (Scale bar = 200 µm). ( C ) Effect of 10 ng/mL EFNA1 on protein aggregates content in matured sheep oocytes. The protein aggregates content was determined by proteoStat aggresome detection reagent labeling (Scale bar = 50 µm). ( D ) Effect of 10 ng/mL EFNA1 on proteasome activity in matured sheep oocytes. The proteasome activity was evaluated by Me4BodipyFL (Scale bar = 50 µm). ( E , F ) Immunofluorescence detection of ER stress-associated protein (GRP78, ATF6) in matured sheep oocytes cultured with or without EFNA1 (Scale bar = 50 µm). ( G ) Relative expression levels of ER stress-associated genes (GRP78, PERK, EIF2A, ATF4, ATF6, IRE1, XBP1) in matured sheep oocytes cultured with or without EFNA1 as analyzed by RT-PCR. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: ROS and GSH levels were evaluated using the ROS assay kit (S0033S, Beyotime, Jiangsu, China) and CMF2HC (HY-D1571, MedChemexpress, Princeton, NJ, USA).

    Techniques: Quantitative Proteomics, Phospho-proteomics, In Vitro, In Vivo, Labeling, Activity Assay, Immunofluorescence, Cell Culture, Expressing, Reverse Transcription Polymerase Chain Reaction