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claudin 1  (Proteintech)


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    Proteintech claudin 1
    HDCA alleviates neuropathic pain and enhances intestinal barrier function. (A) The concentration of total bile acids in fecal samples (n = 6). (B) The expression of HDCA in fecal samples (n = 6). (C) Schedule for the management of HDCA. (D, E) PWT and PWL of mice following HDCA treatment (n = 6). Representative immunoblots (F) and relative expression levels of FXR mRNA (G) and FXR protein (H). (I) Immunohistochemical staining of FXR + cells and Alcian Blue staining in the distal ileum (scale bar, 100 μm, n = 6). (J) Quantitative analysis of FXR + cells. The relative expression levels of ZO-1 (K), occludin (L) <t>and</t> <t>claudin-1</t> (M) protein in distal ileum (n = 3). (N) Representative immunoblots and relative expression levels of Mucin-2 (P) (n = 3). (O) Quantitative analysis levels of the number of goblet cells in the distal ileum (n = 6). (Q, R) Immunofluorescence staining and mean fluorescence intensity of Mucin-2 (scale, 100 μm, n = 3). (S) Transmission electron microscopy examination of the distal ileum. (scale, 1.0 μm). * P < 0.05, ** P < 0.01 by unpaired Student's t‐test (A and B). * P < 0.05, ** P < 0.01 *** P < 0.001, # P < 0.05, ## P < 0.01 by Two-way repeated ANOVA with post hoc Bonferroni’s test (D and E), * P < 0.05, ** P < 0.01, *** P < 0.001 by One-way ANOVA with post hoc Tukey's test (G, H, J–M, O–Q). Data is presented as mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Claudin 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/claudin 1/product/Proteintech
    Average 98 stars, based on 55 article reviews
    claudin 1 - by Bioz Stars, 2026-05
    98/100 stars

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    1) Product Images from "Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling"

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2025.07.017

    HDCA alleviates neuropathic pain and enhances intestinal barrier function. (A) The concentration of total bile acids in fecal samples (n = 6). (B) The expression of HDCA in fecal samples (n = 6). (C) Schedule for the management of HDCA. (D, E) PWT and PWL of mice following HDCA treatment (n = 6). Representative immunoblots (F) and relative expression levels of FXR mRNA (G) and FXR protein (H). (I) Immunohistochemical staining of FXR + cells and Alcian Blue staining in the distal ileum (scale bar, 100 μm, n = 6). (J) Quantitative analysis of FXR + cells. The relative expression levels of ZO-1 (K), occludin (L) and claudin-1 (M) protein in distal ileum (n = 3). (N) Representative immunoblots and relative expression levels of Mucin-2 (P) (n = 3). (O) Quantitative analysis levels of the number of goblet cells in the distal ileum (n = 6). (Q, R) Immunofluorescence staining and mean fluorescence intensity of Mucin-2 (scale, 100 μm, n = 3). (S) Transmission electron microscopy examination of the distal ileum. (scale, 1.0 μm). * P < 0.05, ** P < 0.01 by unpaired Student's t‐test (A and B). * P < 0.05, ** P < 0.01 *** P < 0.001, # P < 0.05, ## P < 0.01 by Two-way repeated ANOVA with post hoc Bonferroni’s test (D and E), * P < 0.05, ** P < 0.01, *** P < 0.001 by One-way ANOVA with post hoc Tukey's test (G, H, J–M, O–Q). Data is presented as mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: HDCA alleviates neuropathic pain and enhances intestinal barrier function. (A) The concentration of total bile acids in fecal samples (n = 6). (B) The expression of HDCA in fecal samples (n = 6). (C) Schedule for the management of HDCA. (D, E) PWT and PWL of mice following HDCA treatment (n = 6). Representative immunoblots (F) and relative expression levels of FXR mRNA (G) and FXR protein (H). (I) Immunohistochemical staining of FXR + cells and Alcian Blue staining in the distal ileum (scale bar, 100 μm, n = 6). (J) Quantitative analysis of FXR + cells. The relative expression levels of ZO-1 (K), occludin (L) and claudin-1 (M) protein in distal ileum (n = 3). (N) Representative immunoblots and relative expression levels of Mucin-2 (P) (n = 3). (O) Quantitative analysis levels of the number of goblet cells in the distal ileum (n = 6). (Q, R) Immunofluorescence staining and mean fluorescence intensity of Mucin-2 (scale, 100 μm, n = 3). (S) Transmission electron microscopy examination of the distal ileum. (scale, 1.0 μm). * P < 0.05, ** P < 0.01 by unpaired Student's t‐test (A and B). * P < 0.05, ** P < 0.01 *** P < 0.001, # P < 0.05, ## P < 0.01 by Two-way repeated ANOVA with post hoc Bonferroni’s test (D and E), * P < 0.05, ** P < 0.01, *** P < 0.001 by One-way ANOVA with post hoc Tukey's test (G, H, J–M, O–Q). Data is presented as mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Concentration Assay, Expressing, Western Blot, Immunohistochemical staining, Staining, Immunofluorescence, Fluorescence, Transmission Assay, Electron Microscopy



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    Image Search Results


    HDCA alleviates neuropathic pain and enhances intestinal barrier function. (A) The concentration of total bile acids in fecal samples (n = 6). (B) The expression of HDCA in fecal samples (n = 6). (C) Schedule for the management of HDCA. (D, E) PWT and PWL of mice following HDCA treatment (n = 6). Representative immunoblots (F) and relative expression levels of FXR mRNA (G) and FXR protein (H). (I) Immunohistochemical staining of FXR + cells and Alcian Blue staining in the distal ileum (scale bar, 100 μm, n = 6). (J) Quantitative analysis of FXR + cells. The relative expression levels of ZO-1 (K), occludin (L) and claudin-1 (M) protein in distal ileum (n = 3). (N) Representative immunoblots and relative expression levels of Mucin-2 (P) (n = 3). (O) Quantitative analysis levels of the number of goblet cells in the distal ileum (n = 6). (Q, R) Immunofluorescence staining and mean fluorescence intensity of Mucin-2 (scale, 100 μm, n = 3). (S) Transmission electron microscopy examination of the distal ileum. (scale, 1.0 μm). * P < 0.05, ** P < 0.01 by unpaired Student's t‐test (A and B). * P < 0.05, ** P < 0.01 *** P < 0.001, # P < 0.05, ## P < 0.01 by Two-way repeated ANOVA with post hoc Bonferroni’s test (D and E), * P < 0.05, ** P < 0.01, *** P < 0.001 by One-way ANOVA with post hoc Tukey's test (G, H, J–M, O–Q). Data is presented as mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling

    doi: 10.1016/j.jare.2025.07.017

    Figure Lengend Snippet: HDCA alleviates neuropathic pain and enhances intestinal barrier function. (A) The concentration of total bile acids in fecal samples (n = 6). (B) The expression of HDCA in fecal samples (n = 6). (C) Schedule for the management of HDCA. (D, E) PWT and PWL of mice following HDCA treatment (n = 6). Representative immunoblots (F) and relative expression levels of FXR mRNA (G) and FXR protein (H). (I) Immunohistochemical staining of FXR + cells and Alcian Blue staining in the distal ileum (scale bar, 100 μm, n = 6). (J) Quantitative analysis of FXR + cells. The relative expression levels of ZO-1 (K), occludin (L) and claudin-1 (M) protein in distal ileum (n = 3). (N) Representative immunoblots and relative expression levels of Mucin-2 (P) (n = 3). (O) Quantitative analysis levels of the number of goblet cells in the distal ileum (n = 6). (Q, R) Immunofluorescence staining and mean fluorescence intensity of Mucin-2 (scale, 100 μm, n = 3). (S) Transmission electron microscopy examination of the distal ileum. (scale, 1.0 μm). * P < 0.05, ** P < 0.01 by unpaired Student's t‐test (A and B). * P < 0.05, ** P < 0.01 *** P < 0.001, # P < 0.05, ## P < 0.01 by Two-way repeated ANOVA with post hoc Bonferroni’s test (D and E), * P < 0.05, ** P < 0.01, *** P < 0.001 by One-way ANOVA with post hoc Tukey's test (G, H, J–M, O–Q). Data is presented as mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Membranes were blocked using 5 % skim milk at room temperature for 1 h and subsequently incubated at 4 °C overnight with primary antibodies against FXR (1:1000, Immunoway, YN2161), zonula occludens-1 (ZO-1) (1:1000, Affinity, AF5145), Mucin-2 (1:1000, Abcam, ab272692), occludin (1:1000, Proteintech, 27260–1-AP), claudin-1 (1:1000, WL, WL03073), MMP-2 (1:1000, Immunoway, YT2798), MMP-9 (1:1000, Immunoway, YT1892), PPAR-γ (1:1000, Proteintech, 16643-1-AP), NOD-like receptor protein-3 (NLRP3) (1:1000, Immunoway, YT5382), interleukin-6 (IL-6) (1:1000, WL, WL02841), IL-18 (1:1000, WL, WL01127), tumor necrosis factor-alpha (TNF-α) (1:1000, WL, WL01581), IL-10 (1:1000, WL, WL03088), FGF15 (1:500, Santacruz, sc-514647), TGR5 (1:1000, Abcam, ab72608), BSEP (1:1000, Abcam, ab155421), CYP7α1 (1:1000, Abcam, ab65596) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, 60004-1-Ig).

    Techniques: Concentration Assay, Expressing, Western Blot, Immunohistochemical staining, Staining, Immunofluorescence, Fluorescence, Transmission Assay, Electron Microscopy