clad51140  (Cedarlane)

Journal: Journal of Neuroinflammation

Article Title: Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy

doi: 10.1186/1742-2094-10-110

Figure Lengend Snippet: List and features of antibodies

Article Snippet: Anti-PMN , Cedarlane - CLAD51140 , 1:200.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Developmental regulation of the neuroinflammatory responses to LPS and/or hypoxia-ischemia between preterm and term neonates: An experimental study

doi: 10.1186/1742-2094-8-55

Figure Lengend Snippet: P1 versus P12 intracerebral expressions of CINC-1 and levels of neutrophil infiltrations . CINC-1 was detected by ELISA at 4 and 24 h after HI in P1 brains exposed to LPS+/-HI ( A ). No CINC-1 was detected at 48 h after HI in P1 brains exposed or unexposed to LPS+/-HI either by ELISA ( A ) or IHC (data not shown) at 48 h post-HI. In contrast, following HI or LPS+HI at P12, CINC-1 was up-regulated in brains at 4, 24 and 48 h post-HI as shown by ELISA ( A ) and IHC experiments ( B, C ); this increase of CINC-1 intracerebral expression ( C , arrowheads) at P12 was present in the neocortical gray matter ( C ), but not in the underlying white matter. Neutrophils ( E , arrowheads) were detected only at P12 - not at P1 -, at 48 h post LPS+HI, in the neocortex ipsilateral to ischemia, i.e . in the areas of neocortical damage as illustrated in a schema ( D ). Protein detection was performed by ELISA on 3 to 4 brains assayed in duplicate at each time point under each experimental condition. IHC was performed at 48 h post-HI, in 3 to 4 brains under each experimental condition. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA with Newman-Keuls post test, except for LPS+HI versus control at P12 (48 h) by ELISA using t test with Welch correction ( A ).Scale bars = 15 μm ( C, E) .

Article Snippet: Sections were incubated overnight at 4°C with the following primary antibodies directed against the following proteins: IL-1β (1:50; #AAR15G, Serotec, NC, US), TNF-α (1:50; #AB1837P Chemicon, ON, Canada), IL-10 (1:10; #MAB519, R&D System, MN, US), IL-1ra (1:25; #sc-25444, Santa Cruz Biotechnology, CA, US), IL-6 (1:100; #MAB5061, R&D System, MN, US), TGF-β1 (1:100; #sc-146, Santa Cruz Biotechnology, CA, US), MCP-1 (1:50; #AB1834P, Chemicon, ON, Canada), CINC-1 (1:50; #CLLS-B2513, LifeSpan Biosciences, WA, US), CD68 (1:250; #MAB1435, Chemicon, ON, Canada), and with adsorbed antiserum directed against rat neutrophils (1:100; #CLAD51140, Cedarlane, ON, Canada).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Control