Immunohistochemistry:
Virus:Article Title: Identification of the velum interpositum as a meningeal-CNS route for myeloid cell trafficking into the brain.
Article Snippet: Article Identification of the velum interpositum as a meningeal-CNS route for myeloid cell trafficking into the brain Graphical abstract Highlights • The velum interpositum (VI) is a site for myeloid cell entry into the murine brain • The VI is a leptomeningeal extra-parenchymal structure • The VI contains meningeal macrophages and peripheral myeloid cells • Distinct myeloid cells utilize the VI during development and demyelinating disease Authors Lindsay A. Hohsfield, Sung Jin Kim, Rocio A. Barahona, ..., Xiangmin Xu, Thomas E. Lane, Kim N. Green Correspondence lhohsfie@uci.edu (L.A.H.), kngreen@uci.edu (K.N.G.). In brief Hohsfield et al. reveal a novel gateway for immune cells to enter the brain: the velum interpositum.. This structure serves as a crucial entry point for myeloid cells under various brain states.
Recombinant:Article Title: Identification of the velum interpositum as a meningeal-CNS route for myeloid cell trafficking into the brain.
Article Snippet: Article Identification of the velum interpositum as a meningeal-CNS route for myeloid cell trafficking into the brain Graphical abstract Highlights • The velum interpositum (VI) is a site for myeloid cell entry into the murine brain • The VI is a leptomeningeal extra-parenchymal structure • The VI contains meningeal macrophages and peripheral myeloid cells • Distinct myeloid cells utilize the VI during development and demyelinating disease Authors Lindsay A. Hohsfield, Sung Jin Kim, Rocio A. Barahona, ..., Xiangmin Xu, Thomas E. Lane, Kim N. Green Correspondence lhohsfie@uci.edu (L.A.H.), kngreen@uci.edu (K.N.G.). In brief Hohsfield et al. reveal a novel gateway for immune cells to enter the brain: the velum interpositum.. This structure serves as a crucial entry point for myeloid cells under various brain states.
Article Title: TET3 plays a critical role in white adipose development and diet-induced remodeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HSP-90 Cell Signaling Cat# 4874; RRID:AB_2121214 GAPDH Cell Signaling Cat# 2118; RRID:AB_561053 Mouse (G3A1) mAb IgG1 Cell Signaling Cat# 5415; RRID:AB_10829607 FLAG Sigma Cat# F3165; RRID:AB_259529 PDGFRa-PE Invitrogen Cat# 12-1401-81; AB_657615 CD45-APC BD Biosciences Cat# 561018; RRID:AB_10584326 CD31-APC BD Biosciences Cat# 561814; RRID:AB_10893351 TER119-APC BD Biosciences Cat# 561033; RRID:AB_10584336 5mc Abcam Cat# ab10805; RRID:AB_442823 5hmc Active Motif Cat# 39769; RRID:AB_10013602 PPARg Santa Cruz Cat# sc-7273; RRID:AB_628115 MAC-2 Cedarlane Labs Cat# CL8942AP; RRID:AB_10060357 TET3 Millipore Cat# abe383 Chemicals, peptides, and recombinant proteins Insulin Sigma I5500 Dexamethasone Sigma D2915 isobutylmethylxanthine Sigma I5879 Puromycin Sigma P8833 Oil Red O Sigma O0625-100G Methylene blue Ricca Chemical 4880–100 Collagenase type 2 Fisher scientific NC9693955 Recombinant CILP protein Abcam 182802 TRI Reagent Solution Invitrogen AM9738 Dynabeads Protein G Invitrogen 10004D Dynabeads anti-mouse IgG Fisher scientific 11-201-D Protein assay dye reagent Bio-Rad 5000006 BSA Sigma A7906 Lipofectamine 3000 Invitrogen L3000-015 Critical commercial assays High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 SYBR green qPCR master mix Bioneer K-6251 mouse leptin ELISA Kit Crystal Chem 90030 ultrasensitive mouse Insulin ELISA kit Crystal Chem 90080 DNeasy Blood & Tissue kit Qiagen 69504 APO-BrdU Fragmentation Assay Kit BioVision K401 RNAqueous -Micro Kit Invitrogen AM1931 ECL Assay Kit PerkinElmer NEL104001EA Dual-Luciferase Reporter Assay System Promega E1980 Hydroxyproline Colorimetric Assay kit BioVision K555 Serum Triglyceride Determination Kit Sigma TR0100 Deposited data Whole genome bisulfite sequencing (WGBS) and RNA-sequencing of preadipocytes from iWAT N/A GSE214483 (Continued on next page) Cell Reports 42, 113196, October 31, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 3T3-L1 ATCC CL-173 HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Pdgfra-Tet3 KO Dr. Anjana Rao and Jackson Laboratory N/A Oligonucleotides qPCR primer sequences Integrated DNA technologies Table S2 gRNA Integrated DNA technologies Table S2 ChIP-qPCR Integrated DNA technologies Table S2 MeDIP Integrated DNA technologies Table S2 Recombinant DNA pM2DG gift from Dr. Didier Trono Addgene, #12259 psPAX2 gift from Dr. Didier Trono Addgene, #12260 pCDH-CMV-MCS-EF1 System Biosciences CD510B-1 pLKO.1 gift from Dr. David Root74 Addgene, #10878 pLN-U6-gRNA Dr. Andreas Stahl N/A pCpGL3-Basic Dr. Michael Rehli N/A Software and algorithms QuantStudio 5 Applied Biosystems A34322 iBrightTM CL1500 Imaging System Invitrogen A44114 ImageJ National Institutes of Health https://imagej.nih.gov/ij/ Adiposoft75 Galarraga et al.75 https://drive.google.com/file/d/ 1TjfoogPQK2NB4VRpZxVn-BgcziCqrS8S/view Hisat276 Kim et al.76 https://github.com/DaehwanKimLab/hisat2 Htseq77 Anders et al.77 https://github.com/simon-anders/htseq Deseq278 Love et al.78 https://github.com/thelovelab/DESeq2 GSEApy79 Fang et al.79 https://github.com/zqfang/GSEApy/tree/v0.9.18 Bismark80 Krueger et al.80 https://github.com/FelixKrueger/Bismark DSS81 Wu et al.81 https://github.com/haowulab/DSS/ GREAT82 McLean et al.82 http://great.stanford.edu/public/html/ Homer83 Heinz, S. et al.83 http://homer.ucsd.edu/homer/ FastQC Andrews, S.84 https://github.com/s-andrews/FastQC TrimGalore Altos Labs https://github.com/FelixKrueger/TrimGalore GraphPad Prism GraphPad https://www.graphpad.com/ Other High fat diet Research Diets D12331i
Saline:Article Title: Identification of the velum interpositum as a meningeal-CNS route for myeloid cell trafficking into the brain.
Article Snippet: Article Identification of the velum interpositum as a meningeal-CNS route for myeloid cell trafficking into the brain Graphical abstract Highlights • The velum interpositum (VI) is a site for myeloid cell entry into the murine brain • The VI is a leptomeningeal extra-parenchymal structure • The VI contains meningeal macrophages and peripheral myeloid cells • Distinct myeloid cells utilize the VI during development and demyelinating disease Authors Lindsay A. Hohsfield, Sung Jin Kim, Rocio A. Barahona, ..., Xiangmin Xu, Thomas E. Lane, Kim N. Green Correspondence lhohsfie@uci.edu (L.A.H.), kngreen@uci.edu (K.N.G.). In brief Hohsfield et al. reveal a novel gateway for immune cells to enter the brain: the velum interpositum.. This structure serves as a crucial entry point for myeloid cells under various brain states.
Incubation:Article Title: Dietary depletion of glutamine is atheroprotective
Article Snippet: The next day the slides were washed with 1× PBS + 0.1% Tween-20 and 1× PBS for five minutes each and incubated in donkey anti-rabbit IgG Alexa Fluor 546 at 1:250 dilution, (ThermoFisher; cat#: A10040). .. After an additional wash step, slides were incubated in rat anti-LGALS3 at 1:500 dilution (Cedarlane; cat#: CL8942AP) for one hour. .. Donkey anti-goat conjugated to Alexa 647 at 1:250 dilution (Invitrogen; cat#: A21447), mouse monoclonal SM α-actin-FITC at 1:250 dilution (ACTA2) (clone 1A4, Sigma Aldrich; cat#: F3337), and DAPI at 1:100 dilution (5 mg/mL; Invitrogen; cat#: D21490) were all added after a wash step and left to incubate for one hour at RT.
Article Title: An mTORC1 ‐Dependent Mouse Model for Cardiac Sarcoidosis
Article Snippet: .. Following citrate antigen retrieval, slides were incubated overnight at 4 °C with primary antibodies: anti‐Galectin 3 (Mac‐2) (Abcam, ab76245 or Cedarlane CL8942AP), rabbit monoclonal anti‐Phospho‐S6 Ribosomal Protein (p‐S6) (Ser240/244) (Cell Signaling Technology, 5364S), rabbit polyclonal anti‐N‐Cadherin (Merck, C3678), rabbit monoclonal anti‐gamma Catenin (plakoglobin) (Abcam, ab184919), rabbit polyclonal anti‐Connexin 43 (Merck, C6219), rabbit monoclonal anti‐CD3 (Abcam, ab5690), CD206 polyclonal antibody (Thermo Fisher PA5‐46994), or rat anti‐CD68 (Thermo Fisher, 14–0681‐82). .. The next day, heart sections were incubated with host‐specific Cy3‐conjugated secondary antibodies and mounted with ProLong Gold (Invitrogen, P36935).
other:
Reverse Transcription:Article Title: TET3 plays a critical role in white adipose development and diet-induced remodeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HSP-90 Cell Signaling Cat# 4874; RRID:AB_2121214 GAPDH Cell Signaling Cat# 2118; RRID:AB_561053 Mouse (G3A1) mAb IgG1 Cell Signaling Cat# 5415; RRID:AB_10829607 FLAG Sigma Cat# F3165; RRID:AB_259529 PDGFRa-PE Invitrogen Cat# 12-1401-81; AB_657615 CD45-APC BD Biosciences Cat# 561018; RRID:AB_10584326 CD31-APC BD Biosciences Cat# 561814; RRID:AB_10893351 TER119-APC BD Biosciences Cat# 561033; RRID:AB_10584336 5mc Abcam Cat# ab10805; RRID:AB_442823 5hmc Active Motif Cat# 39769; RRID:AB_10013602 PPARg Santa Cruz Cat# sc-7273; RRID:AB_628115 MAC-2 Cedarlane Labs Cat# CL8942AP; RRID:AB_10060357 TET3 Millipore Cat# abe383 Chemicals, peptides, and recombinant proteins Insulin Sigma I5500 Dexamethasone Sigma D2915 isobutylmethylxanthine Sigma I5879 Puromycin Sigma P8833 Oil Red O Sigma O0625-100G Methylene blue Ricca Chemical 4880–100 Collagenase type 2 Fisher scientific NC9693955 Recombinant CILP protein Abcam 182802 TRI Reagent Solution Invitrogen AM9738 Dynabeads Protein G Invitrogen 10004D Dynabeads anti-mouse IgG Fisher scientific 11-201-D Protein assay dye reagent Bio-Rad 5000006 BSA Sigma A7906 Lipofectamine 3000 Invitrogen L3000-015 Critical commercial assays High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 SYBR green qPCR master mix Bioneer K-6251 mouse leptin ELISA Kit Crystal Chem 90030 ultrasensitive mouse Insulin ELISA kit Crystal Chem 90080 DNeasy Blood & Tissue kit Qiagen 69504 APO-BrdU Fragmentation Assay Kit BioVision K401 RNAqueous -Micro Kit Invitrogen AM1931 ECL Assay Kit PerkinElmer NEL104001EA Dual-Luciferase Reporter Assay System Promega E1980 Hydroxyproline Colorimetric Assay kit BioVision K555 Serum Triglyceride Determination Kit Sigma TR0100 Deposited data Whole genome bisulfite sequencing (WGBS) and RNA-sequencing of preadipocytes from iWAT N/A GSE214483 (Continued on next page) Cell Reports 42, 113196, October 31, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 3T3-L1 ATCC CL-173 HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Pdgfra-Tet3 KO Dr. Anjana Rao and Jackson Laboratory N/A Oligonucleotides qPCR primer sequences Integrated DNA technologies Table S2 gRNA Integrated DNA technologies Table S2 ChIP-qPCR Integrated DNA technologies Table S2 MeDIP Integrated DNA technologies Table S2 Recombinant DNA pM2DG gift from Dr. Didier Trono Addgene, #12259 psPAX2 gift from Dr. Didier Trono Addgene, #12260 pCDH-CMV-MCS-EF1 System Biosciences CD510B-1 pLKO.1 gift from Dr. David Root74 Addgene, #10878 pLN-U6-gRNA Dr. Andreas Stahl N/A pCpGL3-Basic Dr. Michael Rehli N/A Software and algorithms QuantStudio 5 Applied Biosystems A34322 iBrightTM CL1500 Imaging System Invitrogen A44114 ImageJ National Institutes of Health https://imagej.nih.gov/ij/ Adiposoft75 Galarraga et al.75 https://drive.google.com/file/d/ 1TjfoogPQK2NB4VRpZxVn-BgcziCqrS8S/view Hisat276 Kim et al.76 https://github.com/DaehwanKimLab/hisat2 Htseq77 Anders et al.77 https://github.com/simon-anders/htseq Deseq278 Love et al.78 https://github.com/thelovelab/DESeq2 GSEApy79 Fang et al.79 https://github.com/zqfang/GSEApy/tree/v0.9.18 Bismark80 Krueger et al.80 https://github.com/FelixKrueger/Bismark DSS81 Wu et al.81 https://github.com/haowulab/DSS/ GREAT82 McLean et al.82 http://great.stanford.edu/public/html/ Homer83 Heinz, S. et al.83 http://homer.ucsd.edu/homer/ FastQC Andrews, S.84 https://github.com/s-andrews/FastQC TrimGalore Altos Labs https://github.com/FelixKrueger/TrimGalore GraphPad Prism GraphPad https://www.graphpad.com/ Other High fat diet Research Diets D12331i
SYBR Green Assay:Article Title: TET3 plays a critical role in white adipose development and diet-induced remodeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HSP-90 Cell Signaling Cat# 4874; RRID:AB_2121214 GAPDH Cell Signaling Cat# 2118; RRID:AB_561053 Mouse (G3A1) mAb IgG1 Cell Signaling Cat# 5415; RRID:AB_10829607 FLAG Sigma Cat# F3165; RRID:AB_259529 PDGFRa-PE Invitrogen Cat# 12-1401-81; AB_657615 CD45-APC BD Biosciences Cat# 561018; RRID:AB_10584326 CD31-APC BD Biosciences Cat# 561814; RRID:AB_10893351 TER119-APC BD Biosciences Cat# 561033; RRID:AB_10584336 5mc Abcam Cat# ab10805; RRID:AB_442823 5hmc Active Motif Cat# 39769; RRID:AB_10013602 PPARg Santa Cruz Cat# sc-7273; RRID:AB_628115 MAC-2 Cedarlane Labs Cat# CL8942AP; RRID:AB_10060357 TET3 Millipore Cat# abe383 Chemicals, peptides, and recombinant proteins Insulin Sigma I5500 Dexamethasone Sigma D2915 isobutylmethylxanthine Sigma I5879 Puromycin Sigma P8833 Oil Red O Sigma O0625-100G Methylene blue Ricca Chemical 4880–100 Collagenase type 2 Fisher scientific NC9693955 Recombinant CILP protein Abcam 182802 TRI Reagent Solution Invitrogen AM9738 Dynabeads Protein G Invitrogen 10004D Dynabeads anti-mouse IgG Fisher scientific 11-201-D Protein assay dye reagent Bio-Rad 5000006 BSA Sigma A7906 Lipofectamine 3000 Invitrogen L3000-015 Critical commercial assays High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 SYBR green qPCR master mix Bioneer K-6251 mouse leptin ELISA Kit Crystal Chem 90030 ultrasensitive mouse Insulin ELISA kit Crystal Chem 90080 DNeasy Blood & Tissue kit Qiagen 69504 APO-BrdU Fragmentation Assay Kit BioVision K401 RNAqueous -Micro Kit Invitrogen AM1931 ECL Assay Kit PerkinElmer NEL104001EA Dual-Luciferase Reporter Assay System Promega E1980 Hydroxyproline Colorimetric Assay kit BioVision K555 Serum Triglyceride Determination Kit Sigma TR0100 Deposited data Whole genome bisulfite sequencing (WGBS) and RNA-sequencing of preadipocytes from iWAT N/A GSE214483 (Continued on next page) Cell Reports 42, 113196, October 31, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 3T3-L1 ATCC CL-173 HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Pdgfra-Tet3 KO Dr. Anjana Rao and Jackson Laboratory N/A Oligonucleotides qPCR primer sequences Integrated DNA technologies Table S2 gRNA Integrated DNA technologies Table S2 ChIP-qPCR Integrated DNA technologies Table S2 MeDIP Integrated DNA technologies Table S2 Recombinant DNA pM2DG gift from Dr. Didier Trono Addgene, #12259 psPAX2 gift from Dr. Didier Trono Addgene, #12260 pCDH-CMV-MCS-EF1 System Biosciences CD510B-1 pLKO.1 gift from Dr. David Root74 Addgene, #10878 pLN-U6-gRNA Dr. Andreas Stahl N/A pCpGL3-Basic Dr. Michael Rehli N/A Software and algorithms QuantStudio 5 Applied Biosystems A34322 iBrightTM CL1500 Imaging System Invitrogen A44114 ImageJ National Institutes of Health https://imagej.nih.gov/ij/ Adiposoft75 Galarraga et al.75 https://drive.google.com/file/d/ 1TjfoogPQK2NB4VRpZxVn-BgcziCqrS8S/view Hisat276 Kim et al.76 https://github.com/DaehwanKimLab/hisat2 Htseq77 Anders et al.77 https://github.com/simon-anders/htseq Deseq278 Love et al.78 https://github.com/thelovelab/DESeq2 GSEApy79 Fang et al.79 https://github.com/zqfang/GSEApy/tree/v0.9.18 Bismark80 Krueger et al.80 https://github.com/FelixKrueger/Bismark DSS81 Wu et al.81 https://github.com/haowulab/DSS/ GREAT82 McLean et al.82 http://great.stanford.edu/public/html/ Homer83 Heinz, S. et al.83 http://homer.ucsd.edu/homer/ FastQC Andrews, S.84 https://github.com/s-andrews/FastQC TrimGalore Altos Labs https://github.com/FelixKrueger/TrimGalore GraphPad Prism GraphPad https://www.graphpad.com/ Other High fat diet Research Diets D12331i
Real-time Polymerase Chain Reaction:Article Title: TET3 plays a critical role in white adipose development and diet-induced remodeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HSP-90 Cell Signaling Cat# 4874; RRID:AB_2121214 GAPDH Cell Signaling Cat# 2118; RRID:AB_561053 Mouse (G3A1) mAb IgG1 Cell Signaling Cat# 5415; RRID:AB_10829607 FLAG Sigma Cat# F3165; RRID:AB_259529 PDGFRa-PE Invitrogen Cat# 12-1401-81; AB_657615 CD45-APC BD Biosciences Cat# 561018; RRID:AB_10584326 CD31-APC BD Biosciences Cat# 561814; RRID:AB_10893351 TER119-APC BD Biosciences Cat# 561033; RRID:AB_10584336 5mc Abcam Cat# ab10805; RRID:AB_442823 5hmc Active Motif Cat# 39769; RRID:AB_10013602 PPARg Santa Cruz Cat# sc-7273; RRID:AB_628115 MAC-2 Cedarlane Labs Cat# CL8942AP; RRID:AB_10060357 TET3 Millipore Cat# abe383 Chemicals, peptides, and recombinant proteins Insulin Sigma I5500 Dexamethasone Sigma D2915 isobutylmethylxanthine Sigma I5879 Puromycin Sigma P8833 Oil Red O Sigma O0625-100G Methylene blue Ricca Chemical 4880–100 Collagenase type 2 Fisher scientific NC9693955 Recombinant CILP protein Abcam 182802 TRI Reagent Solution Invitrogen AM9738 Dynabeads Protein G Invitrogen 10004D Dynabeads anti-mouse IgG Fisher scientific 11-201-D Protein assay dye reagent Bio-Rad 5000006 BSA Sigma A7906 Lipofectamine 3000 Invitrogen L3000-015 Critical commercial assays High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 SYBR green qPCR master mix Bioneer K-6251 mouse leptin ELISA Kit Crystal Chem 90030 ultrasensitive mouse Insulin ELISA kit Crystal Chem 90080 DNeasy Blood & Tissue kit Qiagen 69504 APO-BrdU Fragmentation Assay Kit BioVision K401 RNAqueous -Micro Kit Invitrogen AM1931 ECL Assay Kit PerkinElmer NEL104001EA Dual-Luciferase Reporter Assay System Promega E1980 Hydroxyproline Colorimetric Assay kit BioVision K555 Serum Triglyceride Determination Kit Sigma TR0100 Deposited data Whole genome bisulfite sequencing (WGBS) and RNA-sequencing of preadipocytes from iWAT N/A GSE214483 (Continued on next page) Cell Reports 42, 113196, October 31, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 3T3-L1 ATCC CL-173 HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Pdgfra-Tet3 KO Dr. Anjana Rao and Jackson Laboratory N/A Oligonucleotides qPCR primer sequences Integrated DNA technologies Table S2 gRNA Integrated DNA technologies Table S2 ChIP-qPCR Integrated DNA technologies Table S2 MeDIP Integrated DNA technologies Table S2 Recombinant DNA pM2DG gift from Dr. Didier Trono Addgene, #12259 psPAX2 gift from Dr. Didier Trono Addgene, #12260 pCDH-CMV-MCS-EF1 System Biosciences CD510B-1 pLKO.1 gift from Dr. David Root74 Addgene, #10878 pLN-U6-gRNA Dr. Andreas Stahl N/A pCpGL3-Basic Dr. Michael Rehli N/A Software and algorithms QuantStudio 5 Applied Biosystems A34322 iBrightTM CL1500 Imaging System Invitrogen A44114 ImageJ National Institutes of Health https://imagej.nih.gov/ij/ Adiposoft75 Galarraga et al.75 https://drive.google.com/file/d/ 1TjfoogPQK2NB4VRpZxVn-BgcziCqrS8S/view Hisat276 Kim et al.76 https://github.com/DaehwanKimLab/hisat2 Htseq77 Anders et al.77 https://github.com/simon-anders/htseq Deseq278 Love et al.78 https://github.com/thelovelab/DESeq2 GSEApy79 Fang et al.79 https://github.com/zqfang/GSEApy/tree/v0.9.18 Bismark80 Krueger et al.80 https://github.com/FelixKrueger/Bismark DSS81 Wu et al.81 https://github.com/haowulab/DSS/ GREAT82 McLean et al.82 http://great.stanford.edu/public/html/ Homer83 Heinz, S. et al.83 http://homer.ucsd.edu/homer/ FastQC Andrews, S.84 https://github.com/s-andrews/FastQC TrimGalore Altos Labs https://github.com/FelixKrueger/TrimGalore GraphPad Prism GraphPad https://www.graphpad.com/ Other High fat diet Research Diets D12331i
Enzyme-linked Immunosorbent Assay:Article Title: TET3 plays a critical role in white adipose development and diet-induced remodeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HSP-90 Cell Signaling Cat# 4874; RRID:AB_2121214 GAPDH Cell Signaling Cat# 2118; RRID:AB_561053 Mouse (G3A1) mAb IgG1 Cell Signaling Cat# 5415; RRID:AB_10829607 FLAG Sigma Cat# F3165; RRID:AB_259529 PDGFRa-PE Invitrogen Cat# 12-1401-81; AB_657615 CD45-APC BD Biosciences Cat# 561018; RRID:AB_10584326 CD31-APC BD Biosciences Cat# 561814; RRID:AB_10893351 TER119-APC BD Biosciences Cat# 561033; RRID:AB_10584336 5mc Abcam Cat# ab10805; RRID:AB_442823 5hmc Active Motif Cat# 39769; RRID:AB_10013602 PPARg Santa Cruz Cat# sc-7273; RRID:AB_628115 MAC-2 Cedarlane Labs Cat# CL8942AP; RRID:AB_10060357 TET3 Millipore Cat# abe383 Chemicals, peptides, and recombinant proteins Insulin Sigma I5500 Dexamethasone Sigma D2915 isobutylmethylxanthine Sigma I5879 Puromycin Sigma P8833 Oil Red O Sigma O0625-100G Methylene blue Ricca Chemical 4880–100 Collagenase type 2 Fisher scientific NC9693955 Recombinant CILP protein Abcam 182802 TRI Reagent Solution Invitrogen AM9738 Dynabeads Protein G Invitrogen 10004D Dynabeads anti-mouse IgG Fisher scientific 11-201-D Protein assay dye reagent Bio-Rad 5000006 BSA Sigma A7906 Lipofectamine 3000 Invitrogen L3000-015 Critical commercial assays High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 SYBR green qPCR master mix Bioneer K-6251 mouse leptin ELISA Kit Crystal Chem 90030 ultrasensitive mouse Insulin ELISA kit Crystal Chem 90080 DNeasy Blood & Tissue kit Qiagen 69504 APO-BrdU Fragmentation Assay Kit BioVision K401 RNAqueous -Micro Kit Invitrogen AM1931 ECL Assay Kit PerkinElmer NEL104001EA Dual-Luciferase Reporter Assay System Promega E1980 Hydroxyproline Colorimetric Assay kit BioVision K555 Serum Triglyceride Determination Kit Sigma TR0100 Deposited data Whole genome bisulfite sequencing (WGBS) and RNA-sequencing of preadipocytes from iWAT N/A GSE214483 (Continued on next page) Cell Reports 42, 113196, October 31, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 3T3-L1 ATCC CL-173 HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Pdgfra-Tet3 KO Dr. Anjana Rao and Jackson Laboratory N/A Oligonucleotides qPCR primer sequences Integrated DNA technologies Table S2 gRNA Integrated DNA technologies Table S2 ChIP-qPCR Integrated DNA technologies Table S2 MeDIP Integrated DNA technologies Table S2 Recombinant DNA pM2DG gift from Dr. Didier Trono Addgene, #12259 psPAX2 gift from Dr. Didier Trono Addgene, #12260 pCDH-CMV-MCS-EF1 System Biosciences CD510B-1 pLKO.1 gift from Dr. David Root74 Addgene, #10878 pLN-U6-gRNA Dr. Andreas Stahl N/A pCpGL3-Basic Dr. Michael Rehli N/A Software and algorithms QuantStudio 5 Applied Biosystems A34322 iBrightTM CL1500 Imaging System Invitrogen A44114 ImageJ National Institutes of Health https://imagej.nih.gov/ij/ Adiposoft75 Galarraga et al.75 https://drive.google.com/file/d/ 1TjfoogPQK2NB4VRpZxVn-BgcziCqrS8S/view Hisat276 Kim et al.76 https://github.com/DaehwanKimLab/hisat2 Htseq77 Anders et al.77 https://github.com/simon-anders/htseq Deseq278 Love et al.78 https://github.com/thelovelab/DESeq2 GSEApy79 Fang et al.79 https://github.com/zqfang/GSEApy/tree/v0.9.18 Bismark80 Krueger et al.80 https://github.com/FelixKrueger/Bismark DSS81 Wu et al.81 https://github.com/haowulab/DSS/ GREAT82 McLean et al.82 http://great.stanford.edu/public/html/ Homer83 Heinz, S. et al.83 http://homer.ucsd.edu/homer/ FastQC Andrews, S.84 https://github.com/s-andrews/FastQC TrimGalore Altos Labs https://github.com/FelixKrueger/TrimGalore GraphPad Prism GraphPad https://www.graphpad.com/ Other High fat diet Research Diets D12331i
Reporter Assay:Article Title: TET3 plays a critical role in white adipose development and diet-induced remodeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HSP-90 Cell Signaling Cat# 4874; RRID:AB_2121214 GAPDH Cell Signaling Cat# 2118; RRID:AB_561053 Mouse (G3A1) mAb IgG1 Cell Signaling Cat# 5415; RRID:AB_10829607 FLAG Sigma Cat# F3165; RRID:AB_259529 PDGFRa-PE Invitrogen Cat# 12-1401-81; AB_657615 CD45-APC BD Biosciences Cat# 561018; RRID:AB_10584326 CD31-APC BD Biosciences Cat# 561814; RRID:AB_10893351 TER119-APC BD Biosciences Cat# 561033; RRID:AB_10584336 5mc Abcam Cat# ab10805; RRID:AB_442823 5hmc Active Motif Cat# 39769; RRID:AB_10013602 PPARg Santa Cruz Cat# sc-7273; RRID:AB_628115 MAC-2 Cedarlane Labs Cat# CL8942AP; RRID:AB_10060357 TET3 Millipore Cat# abe383 Chemicals, peptides, and recombinant proteins Insulin Sigma I5500 Dexamethasone Sigma D2915 isobutylmethylxanthine Sigma I5879 Puromycin Sigma P8833 Oil Red O Sigma O0625-100G Methylene blue Ricca Chemical 4880–100 Collagenase type 2 Fisher scientific NC9693955 Recombinant CILP protein Abcam 182802 TRI Reagent Solution Invitrogen AM9738 Dynabeads Protein G Invitrogen 10004D Dynabeads anti-mouse IgG Fisher scientific 11-201-D Protein assay dye reagent Bio-Rad 5000006 BSA Sigma A7906 Lipofectamine 3000 Invitrogen L3000-015 Critical commercial assays High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 SYBR green qPCR master mix Bioneer K-6251 mouse leptin ELISA Kit Crystal Chem 90030 ultrasensitive mouse Insulin ELISA kit Crystal Chem 90080 DNeasy Blood & Tissue kit Qiagen 69504 APO-BrdU Fragmentation Assay Kit BioVision K401 RNAqueous -Micro Kit Invitrogen AM1931 ECL Assay Kit PerkinElmer NEL104001EA Dual-Luciferase Reporter Assay System Promega E1980 Hydroxyproline Colorimetric Assay kit BioVision K555 Serum Triglyceride Determination Kit Sigma TR0100 Deposited data Whole genome bisulfite sequencing (WGBS) and RNA-sequencing of preadipocytes from iWAT N/A GSE214483 (Continued on next page) Cell Reports 42, 113196, October 31, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 3T3-L1 ATCC CL-173 HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Pdgfra-Tet3 KO Dr. Anjana Rao and Jackson Laboratory N/A Oligonucleotides qPCR primer sequences Integrated DNA technologies Table S2 gRNA Integrated DNA technologies Table S2 ChIP-qPCR Integrated DNA technologies Table S2 MeDIP Integrated DNA technologies Table S2 Recombinant DNA pM2DG gift from Dr. Didier Trono Addgene, #12259 psPAX2 gift from Dr. Didier Trono Addgene, #12260 pCDH-CMV-MCS-EF1 System Biosciences CD510B-1 pLKO.1 gift from Dr. David Root74 Addgene, #10878 pLN-U6-gRNA Dr. Andreas Stahl N/A pCpGL3-Basic Dr. Michael Rehli N/A Software and algorithms QuantStudio 5 Applied Biosystems A34322 iBrightTM CL1500 Imaging System Invitrogen A44114 ImageJ National Institutes of Health https://imagej.nih.gov/ij/ Adiposoft75 Galarraga et al.75 https://drive.google.com/file/d/ 1TjfoogPQK2NB4VRpZxVn-BgcziCqrS8S/view Hisat276 Kim et al.76 https://github.com/DaehwanKimLab/hisat2 Htseq77 Anders et al.77 https://github.com/simon-anders/htseq Deseq278 Love et al.78 https://github.com/thelovelab/DESeq2 GSEApy79 Fang et al.79 https://github.com/zqfang/GSEApy/tree/v0.9.18 Bismark80 Krueger et al.80 https://github.com/FelixKrueger/Bismark DSS81 Wu et al.81 https://github.com/haowulab/DSS/ GREAT82 McLean et al.82 http://great.stanford.edu/public/html/ Homer83 Heinz, S. et al.83 http://homer.ucsd.edu/homer/ FastQC Andrews, S.84 https://github.com/s-andrews/FastQC TrimGalore Altos Labs https://github.com/FelixKrueger/TrimGalore GraphPad Prism GraphPad https://www.graphpad.com/ Other High fat diet Research Diets D12331i
Colorimetric Assay:Article Title: TET3 plays a critical role in white adipose development and diet-induced remodeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HSP-90 Cell Signaling Cat# 4874; RRID:AB_2121214 GAPDH Cell Signaling Cat# 2118; RRID:AB_561053 Mouse (G3A1) mAb IgG1 Cell Signaling Cat# 5415; RRID:AB_10829607 FLAG Sigma Cat# F3165; RRID:AB_259529 PDGFRa-PE Invitrogen Cat# 12-1401-81; AB_657615 CD45-APC BD Biosciences Cat# 561018; RRID:AB_10584326 CD31-APC BD Biosciences Cat# 561814; RRID:AB_10893351 TER119-APC BD Biosciences Cat# 561033; RRID:AB_10584336 5mc Abcam Cat# ab10805; RRID:AB_442823 5hmc Active Motif Cat# 39769; RRID:AB_10013602 PPARg Santa Cruz Cat# sc-7273; RRID:AB_628115 MAC-2 Cedarlane Labs Cat# CL8942AP; RRID:AB_10060357 TET3 Millipore Cat# abe383 Chemicals, peptides, and recombinant proteins Insulin Sigma I5500 Dexamethasone Sigma D2915 isobutylmethylxanthine Sigma I5879 Puromycin Sigma P8833 Oil Red O Sigma O0625-100G Methylene blue Ricca Chemical 4880–100 Collagenase type 2 Fisher scientific NC9693955 Recombinant CILP protein Abcam 182802 TRI Reagent Solution Invitrogen AM9738 Dynabeads Protein G Invitrogen 10004D Dynabeads anti-mouse IgG Fisher scientific 11-201-D Protein assay dye reagent Bio-Rad 5000006 BSA Sigma A7906 Lipofectamine 3000 Invitrogen L3000-015 Critical commercial assays High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 SYBR green qPCR master mix Bioneer K-6251 mouse leptin ELISA Kit Crystal Chem 90030 ultrasensitive mouse Insulin ELISA kit Crystal Chem 90080 DNeasy Blood & Tissue kit Qiagen 69504 APO-BrdU Fragmentation Assay Kit BioVision K401 RNAqueous -Micro Kit Invitrogen AM1931 ECL Assay Kit PerkinElmer NEL104001EA Dual-Luciferase Reporter Assay System Promega E1980 Hydroxyproline Colorimetric Assay kit BioVision K555 Serum Triglyceride Determination Kit Sigma TR0100 Deposited data Whole genome bisulfite sequencing (WGBS) and RNA-sequencing of preadipocytes from iWAT N/A GSE214483 (Continued on next page) Cell Reports 42, 113196, October 31, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 3T3-L1 ATCC CL-173 HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Pdgfra-Tet3 KO Dr. Anjana Rao and Jackson Laboratory N/A Oligonucleotides qPCR primer sequences Integrated DNA technologies Table S2 gRNA Integrated DNA technologies Table S2 ChIP-qPCR Integrated DNA technologies Table S2 MeDIP Integrated DNA technologies Table S2 Recombinant DNA pM2DG gift from Dr. Didier Trono Addgene, #12259 psPAX2 gift from Dr. Didier Trono Addgene, #12260 pCDH-CMV-MCS-EF1 System Biosciences CD510B-1 pLKO.1 gift from Dr. David Root74 Addgene, #10878 pLN-U6-gRNA Dr. Andreas Stahl N/A pCpGL3-Basic Dr. Michael Rehli N/A Software and algorithms QuantStudio 5 Applied Biosystems A34322 iBrightTM CL1500 Imaging System Invitrogen A44114 ImageJ National Institutes of Health https://imagej.nih.gov/ij/ Adiposoft75 Galarraga et al.75 https://drive.google.com/file/d/ 1TjfoogPQK2NB4VRpZxVn-BgcziCqrS8S/view Hisat276 Kim et al.76 https://github.com/DaehwanKimLab/hisat2 Htseq77 Anders et al.77 https://github.com/simon-anders/htseq Deseq278 Love et al.78 https://github.com/thelovelab/DESeq2 GSEApy79 Fang et al.79 https://github.com/zqfang/GSEApy/tree/v0.9.18 Bismark80 Krueger et al.80 https://github.com/FelixKrueger/Bismark DSS81 Wu et al.81 https://github.com/haowulab/DSS/ GREAT82 McLean et al.82 http://great.stanford.edu/public/html/ Homer83 Heinz, S. et al.83 http://homer.ucsd.edu/homer/ FastQC Andrews, S.84 https://github.com/s-andrews/FastQC TrimGalore Altos Labs https://github.com/FelixKrueger/TrimGalore GraphPad Prism GraphPad https://www.graphpad.com/ Other High fat diet Research Diets D12331i
Methylation Sequencing:Article Title: TET3 plays a critical role in white adipose development and diet-induced remodeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HSP-90 Cell Signaling Cat# 4874; RRID:AB_2121214 GAPDH Cell Signaling Cat# 2118; RRID:AB_561053 Mouse (G3A1) mAb IgG1 Cell Signaling Cat# 5415; RRID:AB_10829607 FLAG Sigma Cat# F3165; RRID:AB_259529 PDGFRa-PE Invitrogen Cat# 12-1401-81; AB_657615 CD45-APC BD Biosciences Cat# 561018; RRID:AB_10584326 CD31-APC BD Biosciences Cat# 561814; RRID:AB_10893351 TER119-APC BD Biosciences Cat# 561033; RRID:AB_10584336 5mc Abcam Cat# ab10805; RRID:AB_442823 5hmc Active Motif Cat# 39769; RRID:AB_10013602 PPARg Santa Cruz Cat# sc-7273; RRID:AB_628115 MAC-2 Cedarlane Labs Cat# CL8942AP; RRID:AB_10060357 TET3 Millipore Cat# abe383 Chemicals, peptides, and recombinant proteins Insulin Sigma I5500 Dexamethasone Sigma D2915 isobutylmethylxanthine Sigma I5879 Puromycin Sigma P8833 Oil Red O Sigma O0625-100G Methylene blue Ricca Chemical 4880–100 Collagenase type 2 Fisher scientific NC9693955 Recombinant CILP protein Abcam 182802 TRI Reagent Solution Invitrogen AM9738 Dynabeads Protein G Invitrogen 10004D Dynabeads anti-mouse IgG Fisher scientific 11-201-D Protein assay dye reagent Bio-Rad 5000006 BSA Sigma A7906 Lipofectamine 3000 Invitrogen L3000-015 Critical commercial assays High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 SYBR green qPCR master mix Bioneer K-6251 mouse leptin ELISA Kit Crystal Chem 90030 ultrasensitive mouse Insulin ELISA kit Crystal Chem 90080 DNeasy Blood & Tissue kit Qiagen 69504 APO-BrdU Fragmentation Assay Kit BioVision K401 RNAqueous -Micro Kit Invitrogen AM1931 ECL Assay Kit PerkinElmer NEL104001EA Dual-Luciferase Reporter Assay System Promega E1980 Hydroxyproline Colorimetric Assay kit BioVision K555 Serum Triglyceride Determination Kit Sigma TR0100 Deposited data Whole genome bisulfite sequencing (WGBS) and RNA-sequencing of preadipocytes from iWAT N/A GSE214483 (Continued on next page) Cell Reports 42, 113196, October 31, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 3T3-L1 ATCC CL-173 HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Pdgfra-Tet3 KO Dr. Anjana Rao and Jackson Laboratory N/A Oligonucleotides qPCR primer sequences Integrated DNA technologies Table S2 gRNA Integrated DNA technologies Table S2 ChIP-qPCR Integrated DNA technologies Table S2 MeDIP Integrated DNA technologies Table S2 Recombinant DNA pM2DG gift from Dr. Didier Trono Addgene, #12259 psPAX2 gift from Dr. Didier Trono Addgene, #12260 pCDH-CMV-MCS-EF1 System Biosciences CD510B-1 pLKO.1 gift from Dr. David Root74 Addgene, #10878 pLN-U6-gRNA Dr. Andreas Stahl N/A pCpGL3-Basic Dr. Michael Rehli N/A Software and algorithms QuantStudio 5 Applied Biosystems A34322 iBrightTM CL1500 Imaging System Invitrogen A44114 ImageJ National Institutes of Health https://imagej.nih.gov/ij/ Adiposoft75 Galarraga et al.75 https://drive.google.com/file/d/ 1TjfoogPQK2NB4VRpZxVn-BgcziCqrS8S/view Hisat276 Kim et al.76 https://github.com/DaehwanKimLab/hisat2 Htseq77 Anders et al.77 https://github.com/simon-anders/htseq Deseq278 Love et al.78 https://github.com/thelovelab/DESeq2 GSEApy79 Fang et al.79 https://github.com/zqfang/GSEApy/tree/v0.9.18 Bismark80 Krueger et al.80 https://github.com/FelixKrueger/Bismark DSS81 Wu et al.81 https://github.com/haowulab/DSS/ GREAT82 McLean et al.82 http://great.stanford.edu/public/html/ Homer83 Heinz, S. et al.83 http://homer.ucsd.edu/homer/ FastQC Andrews, S.84 https://github.com/s-andrews/FastQC TrimGalore Altos Labs https://github.com/FelixKrueger/TrimGalore GraphPad Prism GraphPad https://www.graphpad.com/ Other High fat diet Research Diets D12331i
RNA sequencing:Article Title: TET3 plays a critical role in white adipose development and diet-induced remodeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HSP-90 Cell Signaling Cat# 4874; RRID:AB_2121214 GAPDH Cell Signaling Cat# 2118; RRID:AB_561053 Mouse (G3A1) mAb IgG1 Cell Signaling Cat# 5415; RRID:AB_10829607 FLAG Sigma Cat# F3165; RRID:AB_259529 PDGFRa-PE Invitrogen Cat# 12-1401-81; AB_657615 CD45-APC BD Biosciences Cat# 561018; RRID:AB_10584326 CD31-APC BD Biosciences Cat# 561814; RRID:AB_10893351 TER119-APC BD Biosciences Cat# 561033; RRID:AB_10584336 5mc Abcam Cat# ab10805; RRID:AB_442823 5hmc Active Motif Cat# 39769; RRID:AB_10013602 PPARg Santa Cruz Cat# sc-7273; RRID:AB_628115 MAC-2 Cedarlane Labs Cat# CL8942AP; RRID:AB_10060357 TET3 Millipore Cat# abe383 Chemicals, peptides, and recombinant proteins Insulin Sigma I5500 Dexamethasone Sigma D2915 isobutylmethylxanthine Sigma I5879 Puromycin Sigma P8833 Oil Red O Sigma O0625-100G Methylene blue Ricca Chemical 4880–100 Collagenase type 2 Fisher scientific NC9693955 Recombinant CILP protein Abcam 182802 TRI Reagent Solution Invitrogen AM9738 Dynabeads Protein G Invitrogen 10004D Dynabeads anti-mouse IgG Fisher scientific 11-201-D Protein assay dye reagent Bio-Rad 5000006 BSA Sigma A7906 Lipofectamine 3000 Invitrogen L3000-015 Critical commercial assays High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368813 SYBR green qPCR master mix Bioneer K-6251 mouse leptin ELISA Kit Crystal Chem 90030 ultrasensitive mouse Insulin ELISA kit Crystal Chem 90080 DNeasy Blood & Tissue kit Qiagen 69504 APO-BrdU Fragmentation Assay Kit BioVision K401 RNAqueous -Micro Kit Invitrogen AM1931 ECL Assay Kit PerkinElmer NEL104001EA Dual-Luciferase Reporter Assay System Promega E1980 Hydroxyproline Colorimetric Assay kit BioVision K555 Serum Triglyceride Determination Kit Sigma TR0100 Deposited data Whole genome bisulfite sequencing (WGBS) and RNA-sequencing of preadipocytes from iWAT N/A GSE214483 (Continued on next page) Cell Reports 42, 113196, October 31, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 3T3-L1 ATCC CL-173 HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Pdgfra-Tet3 KO Dr. Anjana Rao and Jackson Laboratory N/A Oligonucleotides qPCR primer sequences Integrated DNA technologies Table S2 gRNA Integrated DNA technologies Table S2 ChIP-qPCR Integrated DNA technologies Table S2 MeDIP Integrated DNA technologies Table S2 Recombinant DNA pM2DG gift from Dr. Didier Trono Addgene, #12259 psPAX2 gift from Dr. Didier Trono Addgene, #12260 pCDH-CMV-MCS-EF1 System Biosciences CD510B-1 pLKO.1 gift from Dr. David Root74 Addgene, #10878 pLN-U6-gRNA Dr. Andreas Stahl N/A pCpGL3-Basic Dr. Michael Rehli N/A Software and algorithms QuantStudio 5 Applied Biosystems A34322 iBrightTM CL1500 Imaging System Invitrogen A44114 ImageJ National Institutes of Health https://imagej.nih.gov/ij/ Adiposoft75 Galarraga et al.75 https://drive.google.com/file/d/ 1TjfoogPQK2NB4VRpZxVn-BgcziCqrS8S/view Hisat276 Kim et al.76 https://github.com/DaehwanKimLab/hisat2 Htseq77 Anders et al.77 https://github.com/simon-anders/htseq Deseq278 Love et al.78 https://github.com/thelovelab/DESeq2 GSEApy79 Fang et al.79 https://github.com/zqfang/GSEApy/tree/v0.9.18 Bismark80 Krueger et al.80 https://github.com/FelixKrueger/Bismark DSS81 Wu et al.81 https://github.com/haowulab/DSS/ GREAT82 McLean et al.82 http://great.stanford.edu/public/html/ Homer83 Heinz, S. et al.83 http://homer.ucsd.edu/homer/ FastQC Andrews, S.84 https://github.com/s-andrews/FastQC TrimGalore Altos Labs https://github.com/FelixKrueger/TrimGalore GraphPad Prism GraphPad https://www.graphpad.com/ Other High fat diet Research Diets D12331i
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