chicken anti myelin p0  (Neuromics)

Journal: Frontiers in Neuroanatomy

Article Title: Large-Scale Convergence of Receptor Cell Arrays Onto Afferent Terminal Arbors in the Lorenzinian Electroreceptors of Polyodon

doi: 10.3389/fnana.2020.00050

Figure Lengend Snippet: Primary antibodies (Ab) used.

Article Snippet: P0 , Protein zero , R , Neuromics , CH23009 , AB_1619444 , >10 , 2.

Techniques: Sequencing

Journal: eLife

Article Title: Deficit of mitogen-activated protein kinase phosphatase 1 (DUSP1) accelerates progressive hearing loss

doi: 10.7554/eLife.39159

Figure Lengend Snippet: Representative microphotographs of hematoxylin-eosin-stained paraffin cochlear mid-modiolar sections of Dusp1 +/+ and Dusp1 –/ – mice showing the spiral ganglion, the organ of Corti and the lateral wall from the middle and basal turns of the cochlea at 2 months of age (n = 3 per genotype). Scale bars: 25 µm. IHC, inner hair cell; OHC, outer hair cell.

Article Snippet: Paraffin cochlear sections (7 μm) were either stained with hematoxylin/eosin or used for immunohistochemical investigations with anti-myelin P0 (chicken, 1:150, CH23009, Neuromics, Edina, MN, USA) and anti-nitrotyrosine (rabbit, 1:100, AB5411, Merck-Millipore, Burlington, MA, USA) ( ).

Techniques: Staining

Journal: eLife

Article Title: Deficit of mitogen-activated protein kinase phosphatase 1 (DUSP1) accelerates progressive hearing loss

doi: 10.7554/eLife.39159

Figure Lengend Snippet: ( A ) Representative microphotographs of hematoxylin-eosin-stained paraffin cochlear mid-modiolar sections of Dusp1 +/+ and Dusp1 –/ – mice, showing the spiral ganglion and the organ of Corti from the middle and basal turns of the cochlea at 4–5 (n = 5 per genotype) and 8–9 months of age (n = 5 per genotype). Insets present representative microphotographs of myelin protein p0 immunohistochemistry from the middle and basal turns of the cochlea at 4–5 (n = 3 per genotype) and 8–9 months of age (n = 3 per genotype). Asterisks and arrowheads indicate the absence of neural and hair cells, respectively. Scale bars: 25 µm. IHC, inner hair cell; OHC, outer hair cell. ( B ) Cochlear gene expression of Mpz , NeuN , Sox2 and Prestin in Dusp1 +/+ (lighter color bars) and Dusp1 –/ – mice (darker color bars) at 2, 4–5 and 8–9 months of age. Expression levels were measured by RT-qPCR and calculated as 2 –ΔΔCt (RQ), using Rplp0 as reference the gene and normalized to the 2-month-old wildtype mice group. Values are presented as mean ± SEM of triplicates from pool samples of three mice per condition. Statistically significant differences were analyzed by Student’s t-tests comparing genotypes (**p<0.01 and ***p<0.001). ( C ) TUNEL apoptosis detection. Representative confocal maximal projection microphotographs show the spiral ganglion of the middle and basal turns of 4–5 month-old Dusp1 +/+ (light green bars, n = 4) and Dusp1 –/ – (dark light bars, n = 3) mice. Arrowheads indicate positive TUNEL cells. Quantification of positive TUNEL cells is shown as the percentage of total DAPI-positive cells in a region of interest (ROI) in the spiral ganglion. Values are presented as mean ± SEM. Statistically significant differences were analyzed by Student’s t-tests comparing genotypes (*p<0.05). Scale bar: 25 µm.

Article Snippet: Paraffin cochlear sections (7 μm) were either stained with hematoxylin/eosin or used for immunohistochemical investigations with anti-myelin P0 (chicken, 1:150, CH23009, Neuromics, Edina, MN, USA) and anti-nitrotyrosine (rabbit, 1:100, AB5411, Merck-Millipore, Burlington, MA, USA) ( ).

Techniques: Staining, Immunohistochemistry, Gene Expression, Expressing, Quantitative RT-PCR, TUNEL Assay

Journal: eLife

Article Title: Deficit of mitogen-activated protein kinase phosphatase 1 (DUSP1) accelerates progressive hearing loss

doi: 10.7554/eLife.39159

Figure Lengend Snippet: ( A ) Representative confocal maximal projection images of the organ of Corti of the middle and basal turns of 5-month-old Dusp1 +/+ (n = 4) and Dusp1 –/ – (n = 4) mice immunolabeled for hair cells MyoVIIA (green), neurofilament (red) and phalloidin (purple). Asterisks and arrowheads indicate the absence or presence, respectively, of hair cells and fibers. Scale bar: 10 µm. IHC, inner hair cell; OHC, outer hair cell; SB, spiral bundle; TC, tunnel of Corti. ( B ) Quantification in the middle (35–45% distance from apex) and basal cochlear turns (60–70% distance from apex) of 5-month-old Dusp1 +/+ (light green bars) and Dusp1 –/ – (dark green bars) mice of the number of outer (base, n = 3 per genotype; middle, n = 4 per genotype) and inner hair cells (base, n = 4 per genotype; middle n = 4, per genotype). Values are presented as mean ± SEM. ( C ) DPOAE thresholds (mean ± SEM) of Dusp1 +/+ (light color lines) and Dusp – / – (dark color lines) mice of 4–5 months of age (8 kHz: Dusp1 +/+ , n = 5, Dusp1 –/ – , n = 8; 10 kHz: Dusp1 +/+ n = 4, Dusp1 –/ – , n = 8; 14 kHz: Dusp1 +/+ n = 4, Dusp1 –/ – n = 7) and 8–9 months of age (8 kHz: Dusp1 +/+ n = 5, Dusp1 –/ – , n = 5; 10 kHz: Dusp1 +/+ n = 4, Dusp1 –/– n = 5; 14 kHz: Dusp1 +/+ n = 4, Dusp1 –/– n = 4). ( D ) DPOAE amplitude I/O function (mean ± SEM) evoked by stimulus (f2 = 10.9 kHz or f2 = 15.2 kHz) of Dusp1 +/+ (lighter color lines) and Dusp1 –/ – (darker color lines) for mice of 4–5 and 8–9 months of age (at least three mice per genotype). Statistically significant differences were analyzed by Student’s t-tests comparing genotypes (*p<0.05, **p<0.01, ***p<0.001).

Article Snippet: Paraffin cochlear sections (7 μm) were either stained with hematoxylin/eosin or used for immunohistochemical investigations with anti-myelin P0 (chicken, 1:150, CH23009, Neuromics, Edina, MN, USA) and anti-nitrotyrosine (rabbit, 1:100, AB5411, Merck-Millipore, Burlington, MA, USA) ( ).

Techniques: Immunolabeling