ch223191  (MedChemExpress)

Journal: Cell & Bioscience

Article Title: Aryl hydrocarbon receptor-mediated transcriptional regulation of HSP70 exacerbates endoplasmic reticulum stress in lupus nephritis

doi: 10.1186/s13578-026-01547-6

Figure Lengend Snippet: Comparison of renal and systemic changes in MRL/lpr mice following AHR inhibitor CH223191 and agonist FICZ treatment. a Drug intervention schedule for MRL/lpr mice. Intraperitoneal injections of PBS, solvent, AHR inhibitor CH223191 , and AHR agonist FICZ were administered to MRL/lpr mice starting at 13 weeks of age. Injections were given every other day for a total of seven doses, followed by a 6-week observation period. Mice were then euthanized at 21 weeks of age for samples collection and analysis. b Characteristic lupus-like skin lesions in different groups of MRL/lpr mice. In the FICZ group, lupus-like skin lesions were first observed around the mouth starting at 17 weeks of age. c Changes in serum ds-DNA and IL-6 levels in different groups of MRL/lpr mice. d Renal pathological changes in PAS staining and transmission electron microscopy (EM) in different MRL/lpr mice groups. Notably, the FICZ intervention group exhibited more pronounced glomerulosclerosis, tubular interstitial inflammation, and endoplasmic reticulum dilation. e Comparison of the glomerulosclerosis index(GSI) and inflammatory cell infiltration index (ICI) in different MRL/lpr mice groups. f Total protein expression of BiP and CHOP in kidneys of different MRL/lpr mice groups. AU means mean gray values of proteins. g Immunofluorescent analysis of BiP and CHOP proteins in C57BL/6 mice and different MRL/lpr mice groups. ns means Not Significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: The AHR agonist FICZ (50ug/kg, i.p. MCE, HY-12451) and inhibitor CH223191 (500ug/kg, i.p. TOPSCIENCE, T2448) were utilized as animal interventions with the assistance of a solvent vehicle [5% DMSO, 40% PEG300, 5% Tween 80 and 50% phosphate-buffered saline (PBS), i.p.], which was selected based on its established tolerability and common use in intraperitoneal drug delivery studies [ ].

Techniques: Comparison, Solvent, Staining, Transmission Assay, Electron Microscopy, Expressing

Journal: Cell & Bioscience

Article Title: Aryl hydrocarbon receptor-mediated transcriptional regulation of HSP70 exacerbates endoplasmic reticulum stress in lupus nephritis

doi: 10.1186/s13578-026-01547-6

Figure Lengend Snippet: Cell proliferation and apoptosis of Bumpt and MGMC cells following AHR inhibitor CH223191 and agonist FICZ treatment under Tunicamycin-induced ER Stress. a Effect of different Tunicamycin concentrations on cell proliferation in Bumpt cells and MGMC under ER stress induction. Bumpt: mouse proximal tubule epithelial cells; MGMC: mouse glomerular mesangial cells. b Effect of different Tunicamycin concentrations on the protein expression of ER stress marker BiP and apoptosis marker CHOP in Bumpt cells and MGMC. Notably, 0.5 µg/ml of Tunicamycin induced significant ER stress and cell apoptosis. c Intervention with 0.01–500 µM concentrations of FICZ and CH223191 in Bumpt cells showed no significant change on cell proliferation. d FICZ (0.1 µM) treatment more significantly inhibits cell proliferation in Bumpt cells and MGMC under 0.5 µg/ml of Tunicamycin (TUN)-induced ER stress. e Annexin-V/PI flow cytometry staining shows a significant increase in apoptosis in Bumpt cells with FICZ (0.1 µM) treatment under 0.5 µg/ml of TUN-induced ER stress. f Protein expression of BiP, CHOP, and ATF4 significantly increased in Bumpt and MGMC cells with FICZ (0.1 µM) treatment under 0.5 µg/ml of TUN-induced ER stress. * p < 0.05, ** p < 0.01, *** p < 0.001 and Not Significant is not labeled on the figure

Article Snippet: The AHR agonist FICZ (50ug/kg, i.p. MCE, HY-12451) and inhibitor CH223191 (500ug/kg, i.p. TOPSCIENCE, T2448) were utilized as animal interventions with the assistance of a solvent vehicle [5% DMSO, 40% PEG300, 5% Tween 80 and 50% phosphate-buffered saline (PBS), i.p.], which was selected based on its established tolerability and common use in intraperitoneal drug delivery studies [ ].

Techniques: Expressing, Marker, Flow Cytometry, Staining, Labeling