fzr1 cdh1 cat no 16368 1 ap  (Proteintech)

Journal: Translational Oncology

Article Title: WISP1 is the stromal-secreting oncoprotein via paracrine downregulation of NDRG1, KAI1, and Maspin in human bladder cancer cells

doi: 10.1016/j.tranon.2026.102680

Figure Lengend Snippet: Determination of the molecular mechanism of WISP1v1 on the cell invasion in the bladder cancer cells. (A) The protein levels of NDRG1, KAI1, and Maspin of HT-DNA and HT-WISP1v1 cells were determined by immunoblot assays. (B) The luciferase activity of the NDRG1 reporter vector was shown after transiently overexpressing various amounts of WISP1v1 expression vectors in HT1376 and T24 cells, as indicated. (C) The luciferase activity of KAI1 and Maspin reporter vectors was shown as indicated after transiently overexpressing various dosages of WISP1v1 expression vectors in HT1376 cells. (D) The luciferase activity of NDRG1, KAI1, and Maspin reporter vectors, respectively, after transiently overexpressed pcDNA, WISP1v1, and WISP1v2 expression vectors as indicated in HT1376 cells. (E) The mRNA levels of the E-cadherin, N-cadherin, Slug, Snail, and Vimentin were determined by RT-qPCR. Data are presented as target genes/β-actin of HT-WISP1v1 cells relative to HT-DNA cells. The mRNA levels of the WISP1 (F), NDRG1, KAI1, Maspin, and IL-6 (G) after transient overexpression of WISP1v1 in T24 cells were determined by RT-qPCR. Data are presented as target genes/β-actin of T24-WISP1v1 cells relative to T24-DNA cells. (H) The invasion ability of T24 cells after the ectopic overexpression of WISP1v1 was determined by in vitro Matrigel invasion assays (± SE; n = 4). The ability of the migration (I) and quantification analysis (I) of T24 cells after being treated with the supernatant from 293T-DNA and 293T-WISP1v1 cells was determined by wound healing assays. The white line indicated the average of the leading cellular edges, and the wound area size was calculated by Image J. * p < 0.05, ** p < 0.01.

Article Snippet: TaqManTM gene expression master mix and polymerase chain reaction (PCR) FAM dye-labeled TaqMan MGB probes for human WISP1 (Hs04234730_m1 for total isoforms and Hs00180245 for WISP1v1), α-SMA (Hs00426835_g1), IL-6 (Hs00985639_m1), GDF15 (Hs00171132_m1), CXCL5 (Hs01099660_g1), SDF-1/CXCL12 (Hs03676656_m1), NDRG1 (Hs00608387_m1), KAI1 (Hs00356310_m1), Maspin (Hs00985283_m1), E-cadherin (Hs01023894_m1), N-cadherin (Hs00169953_m1), Snail (Hs00195591_m1), Slug (Hs00161904_m1), Vimentin (Hs00185584_m1), and β-actin (Hs01060665_g1) were purchased from Thermo Fisher Scientific Inc. (Vilnius, Lithuania).

Techniques: Western Blot, Luciferase, Activity Assay, Plasmid Preparation, Expressing, Quantitative RT-PCR, Over Expression, In Vitro, Migration

Journal: Epigenomes

Article Title: Epigenetic Regulation and Gene Expression Profiles in Cervical Swabs: Toward Non-Invasive Biomarkers of Cervical Lesion Progression

doi: 10.3390/epigenomes10010002

Figure Lengend Snippet: Boxplots of the transformed fold change (FCH) values according to the lesion grade. ( A )— CDH1 , ( B )— BCL2 , ( C )— CD8A , and ( D )— MUC1 . Lesion grades: 1 = control (NILM/negative), 2 = low-grade lesions (ASCUS/LSIL), 3 = high-grade lesions (HSIL). Black line = median, red dot = mean.

Article Snippet: Multiplex quantitative PCR reactions were performed in triplicate using 50 ng of cDNA, TaqMan ® Gene Expression Master Mix (Thermo Fisher Scientific, Waltham, MA, USA), and Taq-Man gene expression assays (BCL2, Hs00153350_m1; CD8A, Hs00233520_m1; MUC1, Hs00904327_m1; GUSB, Hs99999908_m1; CDH1, Hs01013959_m1; ACTB, Hs99999903_m1; and GAPDH, Hs99999905_m1; Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Transformation Assay, Control

Journal: Epigenomes

Article Title: Epigenetic Regulation and Gene Expression Profiles in Cervical Swabs: Toward Non-Invasive Biomarkers of Cervical Lesion Progression

doi: 10.3390/epigenomes10010002

Figure Lengend Snippet: Boxplot of transformed FCH values according to CST status. ( A )—FCH_BCL2, ( B )—FCH_CDH1, ( C )—FCH_CD8A, and ( D )—FCH_MUC1. A lower median expression of BCL2 was observed in CST IV compared with CST III, suggesting downregulation associated with CST IV microbial composition. Black line = median, red dot = mean.

Article Snippet: Multiplex quantitative PCR reactions were performed in triplicate using 50 ng of cDNA, TaqMan ® Gene Expression Master Mix (Thermo Fisher Scientific, Waltham, MA, USA), and Taq-Man gene expression assays (BCL2, Hs00153350_m1; CD8A, Hs00233520_m1; MUC1, Hs00904327_m1; GUSB, Hs99999908_m1; CDH1, Hs01013959_m1; ACTB, Hs99999903_m1; and GAPDH, Hs99999905_m1; Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Transformation Assay, Expressing