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cd81 cst  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cd81 cst
    Cd81 Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd81/CD81+Rabbit+mAb/pm42733113-323-58-59
    Average 95 stars, based on 138 article reviews
    cd81 cst - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Neural stem cell-derived exosomal PA2G4 induces ANXA2 degradation to promote mitophagy and alleviate neuronal oxidative stress in cerebral ischemia/reperfusion.
    Article Snippet: 1 Department of Neurology, The Second Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan, People’s Republic of China 2 Cardiovascular Medicine Intensive Care Unit, The Second Affiliated Hospital of Hainan Medical University, No. 48, Baishuitang Road, Haikou 570311, Hainan, People’s Republic of China Abstract Cerebral ischemia/reperfusion injury (CI/RI) is a common complication of cerebrovascular diseases such as stroke, characterized by mitochondrial dysfunction.. This study investigates the function of proliferation-associated protein 2G4 (PA2G4) released by neural stem cells (NSCs)-derived exosomes (NSC-Exo) in treating middle cerebral artery occlusion/reperfusion (MCAO/R) by regulating mitophagy.. NSC-Exo were extracted and identified.

    Article Title: Heat stress suppresses lactation through potential rumen-mammary communication mediated by extracellular vesicles: integrated analysis of microbiome, metabolome, and miRNA profiles.
    Article Snippet: Equal amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. After blocking with 5% nonfat milk for 2 h at room temperature, membranes were incubated overnight at 4°C with the following primary antibodies against Alix (1:1000 dilution, ab-186429, Abcam; Cambridge, UK), TSG101 (1:1000 dilution, ab-125011, Abcam, Cambridge, UK), CD81 (1:1000 dilution, 52892S, Cell Signaling Technology, Danvers, MA, USA), calnexin (1:1000 dilution, 10427-2-AP, Proteintech, Rosemont, Illinois, USA), and AR TIC LE IN PR ES S HSP70 (1:1000 dilution, ab-181606, Abcam, Cambridge, UK). ..

    Incubation:

    Article Title: Neural stem cell-derived exosomal PA2G4 induces ANXA2 degradation to promote mitophagy and alleviate neuronal oxidative stress in cerebral ischemia/reperfusion.
    Article Snippet: 1 Department of Neurology, The Second Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan, People’s Republic of China 2 Cardiovascular Medicine Intensive Care Unit, The Second Affiliated Hospital of Hainan Medical University, No. 48, Baishuitang Road, Haikou 570311, Hainan, People’s Republic of China Abstract Cerebral ischemia/reperfusion injury (CI/RI) is a common complication of cerebrovascular diseases such as stroke, characterized by mitochondrial dysfunction.. This study investigates the function of proliferation-associated protein 2G4 (PA2G4) released by neural stem cells (NSCs)-derived exosomes (NSC-Exo) in treating middle cerebral artery occlusion/reperfusion (MCAO/R) by regulating mitophagy.. NSC-Exo were extracted and identified.

    Article Title: Exosomes derived from TGF-β1-modified mesenchymal stem cells protect septic mice by inducing macrophage M2 polarization.
    Article Snippet: Equal amounts of protein were separated by 8% or 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. Membranes were blocked with 5% skim milk and incubated overnight at 4 °C with the following primary antibodies: CD9 (Abcam, 1:1000), CD63 (Abcam, 1:2000), and CD81 (Cell Signaling Technology, 1:1000). .. After washing, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary anti-rabbit or anti-mouse antibodies (Beyotime, China, 1:5000).

    Article Title: Cerebrospinal Fluid from Restless Legs Syndrome Patients Reduces Iron Uptake in Blood-Brain Barrier Endothelial Cells by Disrupting the Regulation of Transferrin Receptors.
    Article Snippet: .. The blocked PVDF membrane probed with TfR1 (Santa Cruz,USA, sc-65882, 1:250), FPN1 (Alpha Diagnostic International, USA, MTP-11S, 1:1,000), ferritin heavy chain (FTH1) (Cell Signaling Technology, USA, 4393S, 1:1,000), ferritin light chain (FTL) (Abcam, USA, ab69090; 1:1,000), CD63 (Thermo Fisher Scientific, 1:1,000), CD81 (cell signaling technology, 1:1000, 52892) IRP1and 2 (Cell Signaling Technology, 20272, 37135; 1:1,000), and β-actin (Sigma A544, 1:1,000), incubated overnight at 4 C. The probed membranes were washed with 0.1% TBST, and horseradish peroxidase -conjugated mouse or rabbit secondary antibodies were added and incubated for 1 hour at room temperature. .. The enhanced chemiluminescence (Santa Cruz) reagent was used to see the bands by using Amersham Imager 600 (GE Amersham, United Kingdom).

    Article Title: Exosomes derived from TGF-β1-modified mesenchymal stem cells protect septic mice by inducing macrophage M2 polarization
    Article Snippet: Equal amounts of protein were separated by 8% or 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. Membranes were blocked with 5% skim milk and incubated overnight at 4 °C with the following primary antibodies: CD9 (Abcam, 1:1000), CD63 (Abcam, 1:2000), and CD81 (Cell Signaling Technology, 1:1000). .. After washing, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary anti-rabbit or anti-mouse antibodies (Beyotime, China, 1:5000).

    Article Title: Heat stress suppresses lactation through potential rumen-mammary communication mediated by extracellular vesicles: integrated analysis of microbiome, metabolome, and miRNA profiles.
    Article Snippet: Equal amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. After blocking with 5% nonfat milk for 2 h at room temperature, membranes were incubated overnight at 4°C with the following primary antibodies against Alix (1:1000 dilution, ab-186429, Abcam; Cambridge, UK), TSG101 (1:1000 dilution, ab-125011, Abcam, Cambridge, UK), CD81 (1:1000 dilution, 52892S, Cell Signaling Technology, Danvers, MA, USA), calnexin (1:1000 dilution, 10427-2-AP, Proteintech, Rosemont, Illinois, USA), and AR TIC LE IN PR ES S HSP70 (1:1000 dilution, ab-181606, Abcam, Cambridge, UK). ..

    Cell-Signaling:

    Article Title: ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells
    Article Snippet: .. Finally, the presence of the characteristic EV markers Alix (92880, Cell Signaling Technology), CD81 (56039, Cell Signaling Technology) and TSG101 (sc-7964, Santa Cruz Biotechnology) was confirmed by Western blot analysis. ..

    Western Blot:

    Article Title: ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells
    Article Snippet: .. Finally, the presence of the characteristic EV markers Alix (92880, Cell Signaling Technology), CD81 (56039, Cell Signaling Technology) and TSG101 (sc-7964, Santa Cruz Biotechnology) was confirmed by Western blot analysis. ..

    Article Title: AQP4-IgG-Induced Astrocyte-Derived Small Extracellular Vesicles Carrying Mitochondrial DNA Regulate the TLR9/MyD88/NF-κB Pathway to Drive Microglial Activation and Neuromyelitis Optica.
    Article Snippet: Neuromyelitis optica (NMO) is a central nervous system (CNS) autoimmune disease mediated by pathogenic autoantibodies against aquaporin-4 immunoglobulin G (AQP4IgG) [1].. Astrocyte lysis and reactive astrogliosis are major pathological features during the acute phase of NMO, especially in patients who are seropositive for AQP4-IgG [2, 3].. AQP4-IgG stimulates downstream inflammatory responses through both complement-dependent and complement-independent pathways, resulting in blood-brain barrier disruption, neuroinflammation, demyelination, axonal injury, and Heng Meng menghengjinan@163.com

    Analysis:

    Article Title: ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells
    Article Snippet: .. Finally, the presence of the characteristic EV markers Alix (92880, Cell Signaling Technology), CD81 (56039, Cell Signaling Technology) and TSG101 (sc-7964, Santa Cruz Biotechnology) was confirmed by Western blot analysis. ..

    Membrane:

    Article Title: Cerebrospinal Fluid from Restless Legs Syndrome Patients Reduces Iron Uptake in Blood-Brain Barrier Endothelial Cells by Disrupting the Regulation of Transferrin Receptors.
    Article Snippet: .. The blocked PVDF membrane probed with TfR1 (Santa Cruz,USA, sc-65882, 1:250), FPN1 (Alpha Diagnostic International, USA, MTP-11S, 1:1,000), ferritin heavy chain (FTH1) (Cell Signaling Technology, USA, 4393S, 1:1,000), ferritin light chain (FTL) (Abcam, USA, ab69090; 1:1,000), CD63 (Thermo Fisher Scientific, 1:1,000), CD81 (cell signaling technology, 1:1000, 52892) IRP1and 2 (Cell Signaling Technology, 20272, 37135; 1:1,000), and β-actin (Sigma A544, 1:1,000), incubated overnight at 4 C. The probed membranes were washed with 0.1% TBST, and horseradish peroxidase -conjugated mouse or rabbit secondary antibodies were added and incubated for 1 hour at room temperature. .. The enhanced chemiluminescence (Santa Cruz) reagent was used to see the bands by using Amersham Imager 600 (GE Amersham, United Kingdom).

    Expressing:

    Article Title: AQP4-IgG-Induced Astrocyte-Derived Small Extracellular Vesicles Carrying Mitochondrial DNA Regulate the TLR9/MyD88/NF-κB Pathway to Drive Microglial Activation and Neuromyelitis Optica.
    Article Snippet: Neuromyelitis optica (NMO) is a central nervous system (CNS) autoimmune disease mediated by pathogenic autoantibodies against aquaporin-4 immunoglobulin G (AQP4IgG) [1].. Astrocyte lysis and reactive astrogliosis are major pathological features during the acute phase of NMO, especially in patients who are seropositive for AQP4-IgG [2, 3].. AQP4-IgG stimulates downstream inflammatory responses through both complement-dependent and complement-independent pathways, resulting in blood-brain barrier disruption, neuroinflammation, demyelination, axonal injury, and Heng Meng menghengjinan@163.com



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