Journal: Stem cell research & therapy

Article Title: Exosomes derived from hypoxic mesenchymal stem cells restore ovarian function by enhancing angiogenesis.

doi: 10.1186/s13287-024-04111-6

Figure Lengend Snippet: Fig. 2 Characterization and internalization of norm-Exos and hypo-Exos. A. Morphology of norm-Exos and hypo-Exos observed using TEM. B. Particle size distributions were determined by NTA. C. Comparison of the mean diameters of norm-Exos and hypo-Exos. D. The exosomal surface markers (TSG101, CD9, CD63, and CD81) in norm-Exos and hypo-Exos were assessed by western blotting. E. Semiquantitative analysis of the protein levels of TSG101, CD9, CD63, and CD81. F. Exosomal protein concentrations in norm-Exos and hypo-Exos were analysed using the BCA assay. G. Uptake of PKH67-labelled norm-Exos and hypo-Exos into ROMECs at 24 h. H. Statistical evaluation of fluorescence intensities in the norm-Exo and hypo-Exo groups. n = 3 per group, *P < 0.05 for all figures

Article Snippet: The resulting protein bands were visualized by the enhanced chemiluminescence detection system (Millipore, USA) The main antibodies used in this study are as follows: Calnexin (1:2000, Affinity, China), TSG101 (1:2000, Proteintech, China), CD9 (1:1000, Proteintech, China), CD63 (1:1000, Proteintech, China), CD81 (1:1000, Proteintech, China), GAPDH (1:5000, Proteintech, China), PTEN (1:2000, Affinity, China), PI3K (1:2000, Cell Signaling Technology, USA), p-PI3K (1:2000, Cell Signaling Technology, USA), AKT (1:2000, Cell Signaling Technology, USA), p-AKT (1:2000, Cell Signaling Technology, USA), mTOR (1:2000, Cell Signaling Technology, USA), and p-mTOR (1:2000, Cell Signaling Technology, USA).

Techniques: Comparison, Western Blot, BIA-KA, Fluorescence