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cd49d antibody, anti-mouse  (Miltenyi Biotec)


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    Miltenyi Biotec cd49d antibody, anti-mouse
    Cd49d Antibody, Anti Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd49d/CD49d+Antibody%2C+anti-mouse/custom%40130-121-444%4042385703
    Average 92 stars, based on 34 article reviews
    cd49d antibody, anti-mouse - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Residual homing of α4β7-expressing β1 + PI16 + regulatory T cells with potent suppressive activity correlates with exposure-efficacy of vedolizumab.
    Article Snippet: .. Flow cytometry was performed according to standard protocols using the following fluorochrome- conjugated extracellular antibodies: CD3 (VioGreen, REA613, Miltenyi Biotec), CD4 (FITC/VioBlue/VioGreen/APC- Vio770, VIT4, Miltenyi Biotec), CD45RO (BV510, UCHL1, Biolegend), CD25 (PE/Cy7, BC96, Biolegend), CD127 (APC- Vio770/VioBright FITC, REA614, Miltenyi Biotec; APC, A019D5, Biolegend), CD49d (VioBlue/ FITC, MZ18- 24A9, Miltenyi Biotec; PE/Cy7, 9F10, Biolegend), integrin beta 7 (PerCP/Cy5.5/PE, FIB27, Biolegend; BV605, FIB504, BD BioSciences), integrin beta 1/CD29 (PE/PerCP/ Cy5.5, TS2/16, Biolegend), PI16 (PE/VioBright FITC, REA699, Miltenyi Biotec), GITR (APC, 108–17, Biolegend), CD8 (PerCP/ Cy5.5, RPA- T8, Biolegend), CD19 (VioBlue, Miltenyi Biotec), CD16 (APC/Cy7, 3G8, Biolegend), CD14 (AF488, HCD14, Biolegend), CD56 (PE- Vio770, REA196, Miltenyi Biotec), CCR3 (FITC, 5E8, Biolegend), Siglec 8 (PE- Dazzle594, 7C9, Biolegend). .. Where indicated, vedolizumab (Entyvio, Takeda) and MAdCAM- 1 (rh Fc Chimera Protein, R&D Systems) were labelled using Alexa Fluor Antibody Labelling Kits (AF674/ AF488, Life Technologies) according to the manufacturer’s instructions and used for staining.

    Article Title: Engineering therapeutic T regs to overexpress GPR15 improves functional fitness for in vivo gut homing.
    Article Snippet: .. Flow cytometry Jo urn al Pr e-p roo f To analyze GPR15 and a4b7 integrin expression engineered Tregs were stained with Fixable Viability Dye efluor506 (Invitrogen) for 30 minutes at 4°C, washed once with PBS and stained with the following antibodies for 15 minutes at 4°C: CD25 (APC/Cy7, A251, Biolegend), CD127 (APC, A019D5, Biolegend), CD49d (alpha4; FITC, MZ1824A9, Miltenyi Biotec), integrin beta1 (PerCP/Cy5.5, TS2/16, Biolegend), integrin beta7 (PerCP/Cy5.5, FIB27, Biolegend), GPR15 (BV421, SA302A10, Biolegend). .. Cells were washed once with PBS, fixed overnight at 4°C using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) and intracellularly stained with an antibody against Foxp3 (PE, 236A/E7, Invitrogen) or an isotype control (PE, mouse IgG1 κ P3.6.2.8.1, eBioscience) for 30 minutes at 4°C.

    Article Title: Etrolizumab-s Does Not Induce Residual Trafficking of Regulatory T Cells.
    Article Snippet: From the *Department of Medicine 1, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany †Deutsches Zentrum Immuntherapie (DZI), University Hospital Erlangen, Germany Address correspondence to: Sebastian Zundler, MD, PhD, Department of Medicine 1, University Hospital Erlangen, Friedrich-Alexander-Universität ErlangenNürnberg, Ulmenweg 18, D-91054 Erlangen, Germany (sebastian.zundler@uk-erlangen.de).

    Recombinase Polymerase Amplification:

    Article Title: Residual homing of α4β7-expressing β1 + PI16 + regulatory T cells with potent suppressive activity correlates with exposure-efficacy of vedolizumab.
    Article Snippet: .. Flow cytometry was performed according to standard protocols using the following fluorochrome- conjugated extracellular antibodies: CD3 (VioGreen, REA613, Miltenyi Biotec), CD4 (FITC/VioBlue/VioGreen/APC- Vio770, VIT4, Miltenyi Biotec), CD45RO (BV510, UCHL1, Biolegend), CD25 (PE/Cy7, BC96, Biolegend), CD127 (APC- Vio770/VioBright FITC, REA614, Miltenyi Biotec; APC, A019D5, Biolegend), CD49d (VioBlue/ FITC, MZ18- 24A9, Miltenyi Biotec; PE/Cy7, 9F10, Biolegend), integrin beta 7 (PerCP/Cy5.5/PE, FIB27, Biolegend; BV605, FIB504, BD BioSciences), integrin beta 1/CD29 (PE/PerCP/ Cy5.5, TS2/16, Biolegend), PI16 (PE/VioBright FITC, REA699, Miltenyi Biotec), GITR (APC, 108–17, Biolegend), CD8 (PerCP/ Cy5.5, RPA- T8, Biolegend), CD19 (VioBlue, Miltenyi Biotec), CD16 (APC/Cy7, 3G8, Biolegend), CD14 (AF488, HCD14, Biolegend), CD56 (PE- Vio770, REA196, Miltenyi Biotec), CCR3 (FITC, 5E8, Biolegend), Siglec 8 (PE- Dazzle594, 7C9, Biolegend). .. Where indicated, vedolizumab (Entyvio, Takeda) and MAdCAM- 1 (rh Fc Chimera Protein, R&D Systems) were labelled using Alexa Fluor Antibody Labelling Kits (AF674/ AF488, Life Technologies) according to the manufacturer’s instructions and used for staining.

    Expressing:

    Article Title: Engineering therapeutic T regs to overexpress GPR15 improves functional fitness for in vivo gut homing.
    Article Snippet: .. Flow cytometry Jo urn al Pr e-p roo f To analyze GPR15 and a4b7 integrin expression engineered Tregs were stained with Fixable Viability Dye efluor506 (Invitrogen) for 30 minutes at 4°C, washed once with PBS and stained with the following antibodies for 15 minutes at 4°C: CD25 (APC/Cy7, A251, Biolegend), CD127 (APC, A019D5, Biolegend), CD49d (alpha4; FITC, MZ1824A9, Miltenyi Biotec), integrin beta1 (PerCP/Cy5.5, TS2/16, Biolegend), integrin beta7 (PerCP/Cy5.5, FIB27, Biolegend), GPR15 (BV421, SA302A10, Biolegend). .. Cells were washed once with PBS, fixed overnight at 4°C using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) and intracellularly stained with an antibody against Foxp3 (PE, 236A/E7, Invitrogen) or an isotype control (PE, mouse IgG1 κ P3.6.2.8.1, eBioscience) for 30 minutes at 4°C.

    Staining:

    Article Title: Engineering therapeutic T regs to overexpress GPR15 improves functional fitness for in vivo gut homing.
    Article Snippet: .. Flow cytometry Jo urn al Pr e-p roo f To analyze GPR15 and a4b7 integrin expression engineered Tregs were stained with Fixable Viability Dye efluor506 (Invitrogen) for 30 minutes at 4°C, washed once with PBS and stained with the following antibodies for 15 minutes at 4°C: CD25 (APC/Cy7, A251, Biolegend), CD127 (APC, A019D5, Biolegend), CD49d (alpha4; FITC, MZ1824A9, Miltenyi Biotec), integrin beta1 (PerCP/Cy5.5, TS2/16, Biolegend), integrin beta7 (PerCP/Cy5.5, FIB27, Biolegend), GPR15 (BV421, SA302A10, Biolegend). .. Cells were washed once with PBS, fixed overnight at 4°C using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) and intracellularly stained with an antibody against Foxp3 (PE, 236A/E7, Invitrogen) or an isotype control (PE, mouse IgG1 κ P3.6.2.8.1, eBioscience) for 30 minutes at 4°C.

    Article Title: Etrolizumab-s Does Not Induce Residual Trafficking of Regulatory T Cells.
    Article Snippet: From the *Department of Medicine 1, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany †Deutsches Zentrum Immuntherapie (DZI), University Hospital Erlangen, Germany Address correspondence to: Sebastian Zundler, MD, PhD, Department of Medicine 1, University Hospital Erlangen, Friedrich-Alexander-Universität ErlangenNürnberg, Ulmenweg 18, D-91054 Erlangen, Germany (sebastian.zundler@uk-erlangen.de).

    Article Title: Flow cytometric immunophenotypic differentiation patterns of bone marrow eosinophilopoiesis.
    Article Snippet: For surface immunostaining, bone marrow aspirates or peripheral blood were lysed using FACS lysing buffer (BD Bioscience) and cells were washed twice with 1% albumin in PBS. .. Cells were then stained using directly-conjugated antibodies against CD9, CD11b,CD13, CD34, CD38, CD69, CD44, CD49d, and HLA-DR (Beckman Coulter, Miami, FL), CCR3, CD11c, CD11a, CD16, CD45, CD54, CD62L, CD117, IL-5Rα, and Ki-67 (Becton Dickinson, San Jose, CA), Siglec-8 (BioLegend, San Diego, CA), CD49f and CD162 (Miltenyi, San Diego, CA), and EMR-1 (Bio-Rad, Hercules, CA) before fixation with a Fixative Stabilizer Buffer (Becton Dickinson, San Jose, CA). .. In cases where intracellular staining with Ki-67 was performed, the cells were fixed and permeabilized after the surface staining using the Invitrogen Intracellular staining (ICS) kit (Invitrogen, Waltham, MA) per the manufacturer's protocol and then stained with anti-Ki-67 antibody or matched isotype (Becton Dickinson, San Jose, CA).

    Blocking Assay:

    Article Title: Etrolizumab-s Does Not Induce Residual Trafficking of Regulatory T Cells.
    Article Snippet: From the *Department of Medicine 1, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany †Deutsches Zentrum Immuntherapie (DZI), University Hospital Erlangen, Germany Address correspondence to: Sebastian Zundler, MD, PhD, Department of Medicine 1, University Hospital Erlangen, Friedrich-Alexander-Universität ErlangenNürnberg, Ulmenweg 18, D-91054 Erlangen, Germany (sebastian.zundler@uk-erlangen.de).

    Selection:

    Article Title: Myeloperoxidase-dependent tyrosine halogenation potentiates α-defensins functions
    Article Snippet: Subsequently, neutrophils were purified by an in-house negative selection kit based on a modified mouse-neutrophil positive selection kit EasySep (Stemcell Technologies). .. The antibodies used for the negative selection were: mouse ant-rat CD32 (Becton Dickinson), mouse anti-rat-Ly76 (Miltenyi Biotech), APC conjugated mouse anti-rat antibodies: MHC class II, CD3, CD31, CD4, CD45RC, CD49d, CD8a (Miltenyi Biotech) and CD235a (LifeSpan Biosciences). ..

    Immunopeptidomics:

    Article Title: Myeloperoxidase-dependent tyrosine halogenation potentiates α-defensins functions
    Article Snippet: Subsequently, neutrophils were purified by an in-house negative selection kit based on a modified mouse-neutrophil positive selection kit EasySep (Stemcell Technologies). .. The antibodies used for the negative selection were: mouse ant-rat CD32 (Becton Dickinson), mouse anti-rat-Ly76 (Miltenyi Biotech), APC conjugated mouse anti-rat antibodies: MHC class II, CD3, CD31, CD4, CD45RC, CD49d, CD8a (Miltenyi Biotech) and CD235a (LifeSpan Biosciences). ..



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    Image Search Results


    qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and CD49d, grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.

    Journal: Scientific Reports

    Article Title: Choice of lipid supplementation for in vitro erythroid cell culture impacts reticulocyte yield and characteristics

    doi: 10.1038/s41598-026-37229-z

    Figure Lengend Snippet: qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and CD49d, grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.

    Article Snippet: CD49d was detected using a FITC-conjugated anti-human CD49d antibody (clone MZ18-24A9), a mouse IgG2b from Miltenyi Biotech.

    Techniques: Clinical Proteomics, Cell Culture, Binding Assay, Isolation, Standard Deviation