Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Pneumolysin-induced lung injury is independent of leukocyte trafficking into the alveolar space.

doi: 10.4049/jimmunol.173.2.1307

Figure Lengend Snippet: FIGURE 4. Effect of adhesion-blocking Abs or transient neutropenia on PLY-induced neutrophilic alveolitis and lung permeability increase in in- tact mice. A, Mice were either left untreated (0-h time point) or treated with PLY in the absence of function-blocking Abs (40 ng/mouse; f) or received i.v. injections of function-blocking anti-CD18 Abs () or anti-CD18 plus anti-CD49d Abs (v) or were pretreated with anti-Gr-1 to induce neutro- penia (z) followed by intratracheal application of PLY (40 ng/mouse). Mice were sacrificed at the indicated time points and subjected to BAL for determination of BAL fluid leukocyte differentials. B, Mice were either treated with PLY alone (f in B) or were pretreated with anti-CD18 and anti-CD49d Abs (v in B) or were made transiently neutropenic (z in B) followed by PLY application. One hour before sacrifice, mice then re- ceived FITC-labeled albumin i.v. to determine lung vascular leakage. , p 0.01 vs PLY-alone treatment. Values are given as mean SD of five experiments. AU, Arbitrary unit.

Article Snippet: Rat anti-mouse CD49d (VLA-4; clone PS/2; isotype IgG2b) was purchased from Serotec (Munich, Germany).

Techniques: Blocking Assay, Permeability, Labeling

Journal: REPRODUCTION

Article Title: Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium

doi: 10.1530/rep-13-0655

Figure Lengend Snippet: Figure 5 Expression of VCAM1 receptor, integrin a 4 (ITGA4), by bovine conceptuses. (A) Changes in ITGA4 mRNAs in days 17, 20, and 22 conceptuses (P17, P20, and P22 respectively). Note that ITGA4 transcripts were found minimal at the uterine epithelium and stroma. Values represent meanGS.E.M. from three independent samples with duplicates within a day of conceptus collection. **Statistically significant differences in mRNA levels (P!0.01). (B) Immunohisto- chemical analysis of ITGA4 (a, b, c, and d) or VCAM1 (e, f, g, and h) expression in the bovine uterus obtained from day 22 pregnant animals. Tissue sections (10 mm) from day 22 uteri were immunostained for ITGA4 using an anti-ITGA4 antibody (a and c) or normal mouse IgG as a negative control at low (b) and higher magnification (d). (c) Observation of boxed area in B-a at a higher magnification. Tissue sections were immunostained for VCAM1 using an anti-VCAM1 antibody (e and g), or normal rabbit IgG as a negative control at low (f) and higher magnification (h). (g) Observation of boxed area in B-e at a higher magnification. Epi, endometrial luminal epithelium; St, endometrial stroma; Tr, trophoblast. Black scale barZ200 mm and white scale barZ40 mm.

Article Snippet: After 30 min of incubation with 10% normal goat serum, the sections were incubated at 4 8C overnight with a rabbit anti-human VCAM1 polyclonal antibody (1:100 dilution, 0.5 mg/ml, ab106777, Abcam, Cambridge, MA, USA), a mouse anti-human CD49d (ITGA4) antibody (1:100 dilution, 1 mg/ml, MCA697GA, AbD Serotec, Hercules, CA, USA), or normal mouse IgG (1:40 dilution, 0.4 mg/ml, sc-2025, Santa Cruz Biotechnology, Inc.) or normal rabbit IgG (1:80 dilution, 0.4 mg/ml, sc-2027, Santa Cruz Biotechnology, Inc.) as a Reproduction (2014) 148 119–127 A negative control.

Techniques: Expressing, Negative Control

Journal: International journal of stem cells

Article Title: Integrin α 4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction.

doi: 10.15283/ijsc22209

Figure Lengend Snippet: Fig. 2. Integrin α4 ＋ adipose-derived stem cells (ASCs) subpopulation were isolated and sorted from ASCs by fluo- rescence-activated cell sorting. (A) The population of integrin α4 ＋ ASCs were about 5.11% and 70.7% in the pre- and postsorted ASCs, respectively. (B) Immunofluorescent staining revealed integrin α4 were robustly expressed in integrin α4 ＋ ASCs subpopulation. (C) Western blotting showed that the ex- pression of integrin α4 with 135 kDa was obviously more in integrin α4 ＋

Article Snippet: A mouse anti-integrin α4 antibody was used to detect the grafted integrin α4+ ASCs. von Willebrand Factor (vWF), an endothelial cell marker, was identified by an mouse anti-vWF antibody (Proteintech).

Techniques: Derivative Assay, Isolation, FACS, Staining, Western Blot

Journal: International journal of stem cells

Article Title: Integrin α 4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction.

doi: 10.15283/ijsc22209

Figure Lengend Snippet: Fig. 4. Identification of Integrin α4 ＋/green fluorescent protein＋ (GFP＋) adipose-derived stem cells (ASCs) and incorporation of injected ASCs into the vasculature by immunofluorescent staining in infarcted myocardium 4 weeks posttransplantation. (A) Immunofluorescent staining was performed for GFP (green) and integrin α4 (red), and nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, blue), co-localized expression of integrin α4 and GFP were detected in the hearts receiving integrin α4 ＋ ASCs subpopulation, and integrin α4 ＋ ASCs sub- population showed much higher engraftment than unfractionated ASCs. (B) Immunofluorescent staining was conducted for GFP (green) and von Willebrand Factor (vWF, red), and nuclei were stained with DAPI (blue), GFP-positive ASCs directly incorporated into vasculature.

Article Snippet: A mouse anti-integrin α4 antibody was used to detect the grafted integrin α4+ ASCs. von Willebrand Factor (vWF), an endothelial cell marker, was identified by an mouse anti-vWF antibody (Proteintech).

Techniques: Derivative Assay, Injection, Staining, Expressing

Journal: International journal of stem cells

Article Title: Integrin α 4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction.

doi: 10.15283/ijsc22209

Figure Lengend Snippet: Fig. 5. Integrin α4 ＋ adipose-derived stem cells (ASCs) subpopu- lation more robustly migrated and engrafted into the infartced my- ocardium after cell transplantation. (A) Four weeks posttranspla- ntation, green fluorescent protein-positive ASCs were more fre- quently detected in the hearts receiving integrin α4 ＋ ASCs sub- population than in the hearts undergoing unfractionated ASCs treatment. (B) The high reproducibility of the standard curve of re- al-time PCR. Serial dilution (10−2∼10−8) of DNA was made 7 times to construct the standard curve. Each pink circle corresponded to one dilution in one experiment. The blue solid line represented the regression analysis. (C) Four weeks after cell transplantation, in- tegrin α4 ＋ ASCs subpopulation treated rats showed the signifi- cantly greater ASC numbers per heart as compared to unfractio- nated ASCs treated rats. *p＜0.05 vs. unfractionated ASCs.

Article Snippet: A mouse anti-integrin α4 antibody was used to detect the grafted integrin α4+ ASCs. von Willebrand Factor (vWF), an endothelial cell marker, was identified by an mouse anti-vWF antibody (Proteintech).

Techniques: Derivative Assay, Transplantation Assay, Serial Dilution, Construct

Journal: International journal of stem cells

Article Title: Integrin α 4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction.

doi: 10.15283/ijsc22209

Figure Lengend Snippet: Fig. 6. Integrin α4 + adipose-derived stem cells (ASCs) subpopulation more effectively reduced infarct size post-implantation in vivo. (A) Representative tomographic histograms of the 18F-fluorodeoxyglucose (FDG) positron emission tomography images at 4 weeks posttrans- plantation. Yellow colors were indicative of higher tracer uptake, while blue colors stood for lower tracer uptake. (B) Four weeks after transplantation, unfractionated ASCs transplantation markedly elevated the 18F-FDG uptake in apical-septal, apical-anterior, and apex seg- ments as compared with phosphate-buffered saline (PBS) control. Of importance, integrin α4 + ASCs subpopulation implantation further increased the 18F-FDG uptake in apical-septal, apical-inferior, and apex segments compared with unfractionated ASCs injection. (C) Representative transverse, coronal, and sagittal myocardial 18F-FDG images at 4 weeks after cell transplantation. The infarcted area can be visually detected as an area of low glucose metabolism. (D) Four weeks after transplantation, integrin α4 + ASCs subpopulation markedly reduced infarct size as compared to unfractionated ASCs and PBS control. (E) Integrin α4 + ASCs subpopulation significantly increased global 18F-FDG uptake compared with unfractionated ASCs and PBS control. *p＜0.05 vs. unfractionated ASCs. #p＜0.05 vs. PBS.

Article Snippet: A mouse anti-integrin α4 antibody was used to detect the grafted integrin α4+ ASCs. von Willebrand Factor (vWF), an endothelial cell marker, was identified by an mouse anti-vWF antibody (Proteintech).

Techniques: Derivative Assay, In Vivo, Positron Emission Tomography, Transplantation Assay, Saline, Control, Injection

Journal: International journal of stem cells

Article Title: Integrin α 4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction.

doi: 10.15283/ijsc22209

Figure Lengend Snippet: Fig. 7. Integrin α4 ＋ adipose-derived stem cells (ASCs) subpopulation more effectively improve cardiac fucntion post-implantation in vivo. (A, B) Four weeks after transplantation, integrin α4 ＋ ASCs subpopulation reduced left ventricular end-diastolic volume (LVEDV) and left ventricular end-systolic volume (LVESV) compared with unfractionated ASCs and phosphate-buffered saline (PBS) control. (C) Integrin α4 ＋

Article Snippet: A mouse anti-integrin α4 antibody was used to detect the grafted integrin α4+ ASCs. von Willebrand Factor (vWF), an endothelial cell marker, was identified by an mouse anti-vWF antibody (Proteintech).

Techniques: Derivative Assay, In Vivo, Transplantation Assay, Saline, Control

Journal: Journal of Neuroinflammation

Article Title: Extended interval dosing of ocrelizumab modifies the repopulation of B cells without altering the clinical efficacy in multiple sclerosis

doi: 10.1186/s12974-023-02900-z

Figure Lengend Snippet: Antibodies for CyTOF

Article Snippet: 174Yb , CD49d , 9F10 , Standard BioTools , 0.125 , Surface.

Techniques: Concentration Assay