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cd48 pe vio 770  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd48 pe vio 770
    ( A ) Young Pnpla2 fl/fl and Pnpla2 AdipoQΔ/Δ mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs . Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS (Lin − cKIT + Sca-1 + ) and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. (E) Representative flow cytometry plots showing <t>CD48</t> vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ) and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs . CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of (J) MPP3 and (K) MPP4 cells. Data shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=7-11 per group, males & females). p values determined using two-way ANOVA. NS, not significant; *p < 0.05. Data pooled from three independent experiments with a similar trend.
    Cd48 Pe Vio 770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd48/CD48+Antibody%2C+anti-mouse/bio_rxiv__64898__2026__06__27__734955-306-50-53
    Average 93 stars, based on 51 article reviews
    cd48 pe vio 770 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Age-dependent disease tolerance to SARS-CoV-2 infection"

    Article Title: Age-dependent disease tolerance to SARS-CoV-2 infection

    Journal: bioRxiv

    doi: 10.64898/2026.06.27.734955

    ( A ) Young Pnpla2 fl/fl and Pnpla2 AdipoQΔ/Δ mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs . Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS (Lin − cKIT + Sca-1 + ) and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. (E) Representative flow cytometry plots showing CD48 vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ) and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs . CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of (J) MPP3 and (K) MPP4 cells. Data shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=7-11 per group, males & females). p values determined using two-way ANOVA. NS, not significant; *p < 0.05. Data pooled from three independent experiments with a similar trend.
    Figure Legend Snippet: ( A ) Young Pnpla2 fl/fl and Pnpla2 AdipoQΔ/Δ mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs . Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS (Lin − cKIT + Sca-1 + ) and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. (E) Representative flow cytometry plots showing CD48 vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ) and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs . CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of (J) MPP3 and (K) MPP4 cells. Data shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=7-11 per group, males & females). p values determined using two-way ANOVA. NS, not significant; *p < 0.05. Data pooled from three independent experiments with a similar trend.

    Techniques Used: Infection, Flow Cytometry

    ( A ) Young (3 months) and old (>20 months) mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs. Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS cells and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. ( E ) Representative flow cytometry plots showing CD48 vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ), and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs. CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of ( J ) MPP3 and ( K ) MPP4 cells. Data are shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=6 per group, males). p values were determined using two-way ANOVA. NS, not significant; *p < 0.05, **p < 0.01; ***p < 0.001. Data pooled from two independent experiments with a similar trend.
    Figure Legend Snippet: ( A ) Young (3 months) and old (>20 months) mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs. Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS cells and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. ( E ) Representative flow cytometry plots showing CD48 vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ), and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs. CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of ( J ) MPP3 and ( K ) MPP4 cells. Data are shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=6 per group, males). p values were determined using two-way ANOVA. NS, not significant; *p < 0.05, **p < 0.01; ***p < 0.001. Data pooled from two independent experiments with a similar trend.

    Techniques Used: Infection, Flow Cytometry

    Related Articles

    Blocking Assay:

    Article Title: Intrahepatic microbes govern liver immunity by programming NKT cells
    Article Snippet: .. After 8 blocking FcγRIII/II with an anti-CD16/CD32 mAb (eBioscience), cell labeling was 9 performed by incubating 106 cells with 1 μg of fluorescently conjugated antibody 10 directed against murine CD45 (30-F11), CD45.1 (A20), CD45.2 (104), CD11b (M1/70), 11 F4/80 (BM8), Gr-1 (RB6-8C5), CD11c (N418), MHC II (M5/114.15.2), CD80 (16-12 10A1), CD86 (GL-1), CD3 (17A2), TCRβ (H57-597), NK1.1 (PK136), CD48 (HM48-1), 13 CD1d (1B1), CD244 (m2B4[B6]458.1), CCL5 (2E9/CCL5), IL10 (JES5-16E3), IFNγ 14 (XMG1.2), CD4 (RM4-5), CD8a (53-6.7), Vβ2 (B20.6), Vβ7 (TR310; all BioLegend), 15 Vβ8 (REA684; Miltenyi), and FoxP3 (FJK-16s; Invitrogen). .. Cell preparation for 16 intracellular staining was performed using the Fixation and Permeabilization Solution Kit 17 (eBiosciences).

    Labeling:

    Article Title: Intrahepatic microbes govern liver immunity by programming NKT cells
    Article Snippet: .. After 8 blocking FcγRIII/II with an anti-CD16/CD32 mAb (eBioscience), cell labeling was 9 performed by incubating 106 cells with 1 μg of fluorescently conjugated antibody 10 directed against murine CD45 (30-F11), CD45.1 (A20), CD45.2 (104), CD11b (M1/70), 11 F4/80 (BM8), Gr-1 (RB6-8C5), CD11c (N418), MHC II (M5/114.15.2), CD80 (16-12 10A1), CD86 (GL-1), CD3 (17A2), TCRβ (H57-597), NK1.1 (PK136), CD48 (HM48-1), 13 CD1d (1B1), CD244 (m2B4[B6]458.1), CCL5 (2E9/CCL5), IL10 (JES5-16E3), IFNγ 14 (XMG1.2), CD4 (RM4-5), CD8a (53-6.7), Vβ2 (B20.6), Vβ7 (TR310; all BioLegend), 15 Vβ8 (REA684; Miltenyi), and FoxP3 (FJK-16s; Invitrogen). .. Cell preparation for 16 intracellular staining was performed using the Fixation and Permeabilization Solution Kit 17 (eBiosciences).

    Immunopeptidomics:

    Article Title: Intrahepatic microbes govern liver immunity by programming NKT cells
    Article Snippet: .. After 8 blocking FcγRIII/II with an anti-CD16/CD32 mAb (eBioscience), cell labeling was 9 performed by incubating 106 cells with 1 μg of fluorescently conjugated antibody 10 directed against murine CD45 (30-F11), CD45.1 (A20), CD45.2 (104), CD11b (M1/70), 11 F4/80 (BM8), Gr-1 (RB6-8C5), CD11c (N418), MHC II (M5/114.15.2), CD80 (16-12 10A1), CD86 (GL-1), CD3 (17A2), TCRβ (H57-597), NK1.1 (PK136), CD48 (HM48-1), 13 CD1d (1B1), CD244 (m2B4[B6]458.1), CCL5 (2E9/CCL5), IL10 (JES5-16E3), IFNγ 14 (XMG1.2), CD4 (RM4-5), CD8a (53-6.7), Vβ2 (B20.6), Vβ7 (TR310; all BioLegend), 15 Vβ8 (REA684; Miltenyi), and FoxP3 (FJK-16s; Invitrogen). .. Cell preparation for 16 intracellular staining was performed using the Fixation and Permeabilization Solution Kit 17 (eBiosciences).

    Control:

    Article Title: An epigenetic GPI anchor defect impairs TLR4 signaling in the B cell transdifferentiation model for primary human monocytes BLaER1.
    Article Snippet: Differentiated or undifferentiated BLaER1 cells (3 × 105) were washed with FACS buffer (2% FCS, 2 mM EDTA, PBS) once and incubated for 15 min in 25 μl Fc Receptor Blocking Solution (BioLegend, 422302, 1:100 in FACS buffer) at 4 °C. .. 25 μl of isotype control (BioLegend, 400220 or 400120, 1:50) or specific antibody for CD14, CD48, CD55, CD11b, CD45, CD64 (BioLegend, 301808, 336713, 311311, 301309, 368511, 305013, all 1:50), CD36, CD163, or CD191 (Miltenyi Biotec, 130-100-307, 130-100-612, 130-100-360, all 1:50) was added and incubated for 15 min at 4 °C. ..

    Article Title: An epigenetic GPI anchor defect impairs TLR4 signaling in the B cell transdifferentiation model for primary human monocytes BLaER1
    Article Snippet: Differentiated or undifferentiated BLaER1 cells (3 × 10 5 ) were washed with FACS buffer (2% FCS, 2 mM EDTA, PBS) once and incubated for 15 min in 25 μl Fc Receptor Blocking Solution (BioLegend, 422302, 1:100 in FACS buffer) at 4 °C. .. 25 μl of isotype control (BioLegend, 400220 or 400120, 1:50) or specific antibody for CD14, CD48, CD55, CD11b, CD45, CD64 (BioLegend, 301808, 336713, 311311, 301309, 368511, 305013, all 1:50), CD36, CD163, or CD191 (Miltenyi Biotec, 130-100-307, 130-100-612, 130-100-360, all 1:50) was added and incubated for 15 min at 4 °C. ..

    Incubation:

    Article Title: An epigenetic GPI anchor defect impairs TLR4 signaling in the B cell transdifferentiation model for primary human monocytes BLaER1.
    Article Snippet: Differentiated or undifferentiated BLaER1 cells (3 × 105) were washed with FACS buffer (2% FCS, 2 mM EDTA, PBS) once and incubated for 15 min in 25 μl Fc Receptor Blocking Solution (BioLegend, 422302, 1:100 in FACS buffer) at 4 °C. .. 25 μl of isotype control (BioLegend, 400220 or 400120, 1:50) or specific antibody for CD14, CD48, CD55, CD11b, CD45, CD64 (BioLegend, 301808, 336713, 311311, 301309, 368511, 305013, all 1:50), CD36, CD163, or CD191 (Miltenyi Biotec, 130-100-307, 130-100-612, 130-100-360, all 1:50) was added and incubated for 15 min at 4 °C. ..

    Article Title: An epigenetic GPI anchor defect impairs TLR4 signaling in the B cell transdifferentiation model for primary human monocytes BLaER1
    Article Snippet: Differentiated or undifferentiated BLaER1 cells (3 × 10 5 ) were washed with FACS buffer (2% FCS, 2 mM EDTA, PBS) once and incubated for 15 min in 25 μl Fc Receptor Blocking Solution (BioLegend, 422302, 1:100 in FACS buffer) at 4 °C. .. 25 μl of isotype control (BioLegend, 400220 or 400120, 1:50) or specific antibody for CD14, CD48, CD55, CD11b, CD45, CD64 (BioLegend, 301808, 336713, 311311, 301309, 368511, 305013, all 1:50), CD36, CD163, or CD191 (Miltenyi Biotec, 130-100-307, 130-100-612, 130-100-360, all 1:50) was added and incubated for 15 min at 4 °C. ..

    Staining:

    Article Title: NK cells shape the clonal evolution of B-ALL cells by IFN-γ production
    Article Snippet: .. The following antibodies (clones) were used for the staining: CD48 (REA1238), CD112 (829038), CD137L (REA962), CD154 (REA785), CD155 (REA519), TRAIL-R (CD261) (REA1233), Qa-1b (6A8.6F10.1A6), Qa-2a (REA523), H-2Db (REA1198), H-2Kb (REA619), Rae1 (REA723) and Ly6a/Sca-1 (REA422) purchased from Miltenyi Biotec. ..

    Article Title: E2f coordinates the cell cycle and cell fate of hematopoietic progenitors to drive stress myelopoiesis
    Article Snippet: .. The remaining white blood cells were stained with a cocktail of lineage antibodies (Cd3, Cd4, Cd8, B220, Ter119, Mac1 and Gr1) as well as antibodies against Kit, Sca1, Flk2/Flt3, Cd48, Cd150, FcgR, Cd34, Ly6c, Ly6g, Rank and Cd115 (all purchased from eBiosciences) and βchain receptor (Miltenyi). .. Inflammatory status of granulocytes and monocytes was determined by assessing the surface expression of Cd18 and CCR2 (eBiosciences), respectively.

    Article Title: Enhanced solid tumor cell targeting by a neoepitope-encoding oncolytic measles virus combined with CAR therapy
    Article Snippet: For detection of CAR-T and CAR-NK cell counts after the coculture, CAR-T cells were labeled with 1 μM CellTrace Violet and 1 μM CellTrace FarRed according to the manufacturer’s instructions, respectively (Thermo Fisher Scientific, Waltham, MA, USA). .. For evaluation of CAR-T and CAR-NK cell activation following coculture with tumor cells and MV, staining with CD3 (CD3 antibody, VioBlue, anti-human, REAfinity), CD16 (CD16 antibody, anti-human, PerCP-Vio700, REAfinity), OX40 (CD134 antibody, PerCP-Vio700, anti-human, REAfinity), NKG2D (CD314 antibody, PE, anti-human, REAfinity), DNAM-1 (CD226 antibody, PE-Vio770, anti-human, REAfinity), 2B4 (CD244 antibody, APC, anti-human, REAfinity), CD48 (CD48 Antibody, APC-Vio770, anti-human, REAfinity) (all Miltenyi Biotec, Bergisch Gladbach, Germany), and OX40L (CD252 antibody, BV421, anti-human) (Becton Dickinson [BD], New Jersey, USA) was carried out after 72 h of coculture. ..

    other:

    Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging
    Article Snippet: CD48 , REA426 , 50 , 130-106-516 , PE , Miltenyi Biotec.

    Activation Assay:

    Article Title: Enhanced solid tumor cell targeting by a neoepitope-encoding oncolytic measles virus combined with CAR therapy
    Article Snippet: For detection of CAR-T and CAR-NK cell counts after the coculture, CAR-T cells were labeled with 1 μM CellTrace Violet and 1 μM CellTrace FarRed according to the manufacturer’s instructions, respectively (Thermo Fisher Scientific, Waltham, MA, USA). .. For evaluation of CAR-T and CAR-NK cell activation following coculture with tumor cells and MV, staining with CD3 (CD3 antibody, VioBlue, anti-human, REAfinity), CD16 (CD16 antibody, anti-human, PerCP-Vio700, REAfinity), OX40 (CD134 antibody, PerCP-Vio700, anti-human, REAfinity), NKG2D (CD314 antibody, PE, anti-human, REAfinity), DNAM-1 (CD226 antibody, PE-Vio770, anti-human, REAfinity), 2B4 (CD244 antibody, APC, anti-human, REAfinity), CD48 (CD48 Antibody, APC-Vio770, anti-human, REAfinity) (all Miltenyi Biotec, Bergisch Gladbach, Germany), and OX40L (CD252 antibody, BV421, anti-human) (Becton Dickinson [BD], New Jersey, USA) was carried out after 72 h of coculture. ..



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    ( A ) Young Pnpla2 fl/fl and Pnpla2 AdipoQΔ/Δ mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs . Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS (Lin − cKIT + Sca-1 + ) and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. (E) Representative flow cytometry plots showing <t>CD48</t> vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ) and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs . CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of (J) MPP3 and (K) MPP4 cells. Data shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=7-11 per group, males & females). p values determined using two-way ANOVA. NS, not significant; *p < 0.05. Data pooled from three independent experiments with a similar trend.
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    Butyrate reverts inflammatory mediators and enhances adherent‐invasive Escherichia coli (AIEC) phagocytosis in human THP‐1 macrophages. THP‐1 macrophages ( n = 3) were treated with butyrate in the presence or absence of lipopolysaccharide (LPS) + IFN‐γ (0.1 + 20 ng·mL −1 ) for 24 h. (A) CD40 and (B) CD80 geometric mean fluorescence intensity (gMFI) in THP‐1‐macrophages ( n = 3 or n = 5) were determined by flow cytometry. (C) Intracellular E. coli HS (commensal) and CD2‐a (AIEC) strains, normalized to control, were phagocytosed by THP‐1 macrophages treated with 2 m m butyrate (HS: n = 6; CD2‐a: n = 7). (D) mRNA levels of <t>CD48</t> and MARCKS ( n = 3) were determined by RT‐qPCR. Statistical analysis: One‐way ANOVA with Bonferroni post‐test (A, B) and Wilcoxon matched‐pairs signed‐rank test (C, D). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
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    Butyrate reverts inflammatory mediators and enhances adherent‐invasive Escherichia coli (AIEC) phagocytosis in human THP‐1 macrophages. THP‐1 macrophages ( n = 3) were treated with butyrate in the presence or absence of lipopolysaccharide (LPS) + IFN‐γ (0.1 + 20 ng·mL −1 ) for 24 h. (A) CD40 and (B) CD80 geometric mean fluorescence intensity (gMFI) in THP‐1‐macrophages ( n = 3 or n = 5) were determined by flow cytometry. (C) Intracellular E. coli HS (commensal) and CD2‐a (AIEC) strains, normalized to control, were phagocytosed by THP‐1 macrophages treated with 2 m m butyrate (HS: n = 6; CD2‐a: n = 7). (D) mRNA levels of <t>CD48</t> and MARCKS ( n = 3) were determined by RT‐qPCR. Statistical analysis: One‐way ANOVA with Bonferroni post‐test (A, B) and Wilcoxon matched‐pairs signed‐rank test (C, D). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
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    Image Search Results


    ( A ) Young Pnpla2 fl/fl and Pnpla2 AdipoQΔ/Δ mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs . Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS (Lin − cKIT + Sca-1 + ) and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. (E) Representative flow cytometry plots showing CD48 vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ) and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs . CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of (J) MPP3 and (K) MPP4 cells. Data shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=7-11 per group, males & females). p values determined using two-way ANOVA. NS, not significant; *p < 0.05. Data pooled from three independent experiments with a similar trend.

    Journal: bioRxiv

    Article Title: Age-dependent disease tolerance to SARS-CoV-2 infection

    doi: 10.64898/2026.06.27.734955

    Figure Lengend Snippet: ( A ) Young Pnpla2 fl/fl and Pnpla2 AdipoQΔ/Δ mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs . Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS (Lin − cKIT + Sca-1 + ) and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. (E) Representative flow cytometry plots showing CD48 vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ) and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs . CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of (J) MPP3 and (K) MPP4 cells. Data shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=7-11 per group, males & females). p values determined using two-way ANOVA. NS, not significant; *p < 0.05. Data pooled from three independent experiments with a similar trend.

    Article Snippet: The cells were stained with the following primary antibodies: Sca-1 FITC (#122505), Ly6C PerCP/Cy5.5 (#128012), CD115 PE (#135505), CD34 PE/Cy5 (#119311), F4/80 AF647 (#123122), CD11C BV605 (#117333), CD3 Biotin (#100243), CD49b Biotin (#103521), CD90.1 Biotin (#202510), all from Biolegend, CD135 PerCPeF710 (#46-1351-82), CD150 eF450 (#48-1502-82), MHCII eF506 (#69-5321-82) from Invitrogen, CD48 PE-Vio 770 (Miltenyi Biotec S. L, #130-102-363), c-KIT APC (BD Pharmingen, #553356), CD19 Biotin (eBioscience, #553784), for 25 min (4°C).

    Techniques: Infection, Flow Cytometry

    ( A ) Young (3 months) and old (>20 months) mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs. Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS cells and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. ( E ) Representative flow cytometry plots showing CD48 vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ), and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs. CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of ( J ) MPP3 and ( K ) MPP4 cells. Data are shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=6 per group, males). p values were determined using two-way ANOVA. NS, not significant; *p < 0.05, **p < 0.01; ***p < 0.001. Data pooled from two independent experiments with a similar trend.

    Journal: bioRxiv

    Article Title: Age-dependent disease tolerance to SARS-CoV-2 infection

    doi: 10.64898/2026.06.27.734955

    Figure Lengend Snippet: ( A ) Young (3 months) and old (>20 months) mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs. Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS cells and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. ( E ) Representative flow cytometry plots showing CD48 vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ), and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs. CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of ( J ) MPP3 and ( K ) MPP4 cells. Data are shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=6 per group, males). p values were determined using two-way ANOVA. NS, not significant; *p < 0.05, **p < 0.01; ***p < 0.001. Data pooled from two independent experiments with a similar trend.

    Article Snippet: The cells were stained with the following primary antibodies: Sca-1 FITC (#122505), Ly6C PerCP/Cy5.5 (#128012), CD115 PE (#135505), CD34 PE/Cy5 (#119311), F4/80 AF647 (#123122), CD11C BV605 (#117333), CD3 Biotin (#100243), CD49b Biotin (#103521), CD90.1 Biotin (#202510), all from Biolegend, CD135 PerCPeF710 (#46-1351-82), CD150 eF450 (#48-1502-82), MHCII eF506 (#69-5321-82) from Invitrogen, CD48 PE-Vio 770 (Miltenyi Biotec S. L, #130-102-363), c-KIT APC (BD Pharmingen, #553356), CD19 Biotin (eBioscience, #553784), for 25 min (4°C).

    Techniques: Infection, Flow Cytometry

    Butyrate reverts inflammatory mediators and enhances adherent‐invasive Escherichia coli (AIEC) phagocytosis in human THP‐1 macrophages. THP‐1 macrophages ( n = 3) were treated with butyrate in the presence or absence of lipopolysaccharide (LPS) + IFN‐γ (0.1 + 20 ng·mL −1 ) for 24 h. (A) CD40 and (B) CD80 geometric mean fluorescence intensity (gMFI) in THP‐1‐macrophages ( n = 3 or n = 5) were determined by flow cytometry. (C) Intracellular E. coli HS (commensal) and CD2‐a (AIEC) strains, normalized to control, were phagocytosed by THP‐1 macrophages treated with 2 m m butyrate (HS: n = 6; CD2‐a: n = 7). (D) mRNA levels of CD48 and MARCKS ( n = 3) were determined by RT‐qPCR. Statistical analysis: One‐way ANOVA with Bonferroni post‐test (A, B) and Wilcoxon matched‐pairs signed‐rank test (C, D). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

    Journal: The Febs Journal

    Article Title: Butyrate suppresses mucosal inflammation in inflammatory bowel disease primarily through HDAC3 inhibition in monocytes and macrophages

    doi: 10.1111/febs.70289

    Figure Lengend Snippet: Butyrate reverts inflammatory mediators and enhances adherent‐invasive Escherichia coli (AIEC) phagocytosis in human THP‐1 macrophages. THP‐1 macrophages ( n = 3) were treated with butyrate in the presence or absence of lipopolysaccharide (LPS) + IFN‐γ (0.1 + 20 ng·mL −1 ) for 24 h. (A) CD40 and (B) CD80 geometric mean fluorescence intensity (gMFI) in THP‐1‐macrophages ( n = 3 or n = 5) were determined by flow cytometry. (C) Intracellular E. coli HS (commensal) and CD2‐a (AIEC) strains, normalized to control, were phagocytosed by THP‐1 macrophages treated with 2 m m butyrate (HS: n = 6; CD2‐a: n = 7). (D) mRNA levels of CD48 and MARCKS ( n = 3) were determined by RT‐qPCR. Statistical analysis: One‐way ANOVA with Bonferroni post‐test (A, B) and Wilcoxon matched‐pairs signed‐rank test (C, D). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

    Article Snippet: Gene expression was analyzed using TaqMan Gene Expression Assays for SLC16A1 (Hs01560299_m1), HCAR2 (Hs02341584_s1), HDAC3 (Hs00187320_m1), IRF1 (Hs00971965_m1), TFF1 (Hs00907239_m1), DUOX2 (Hs00204187_m1), and LCN2 (Hs01008571_m1), MARCKS (Hs00158993_m1), and CD48 (Hs00381156_m1) (Applied Biosystems, Waltham, MA, USA) or TaqMan primers and probes from Eurogentec (Maastricht, The Netherlands; see Table ).

    Techniques: Fluorescence, Flow Cytometry, Control, Quantitative RT-PCR