Journal: bioRxiv

Article Title: Age-dependent disease tolerance to SARS-CoV-2 infection

doi: 10.64898/2026.06.27.734955

Figure Lengend Snippet: ( A ) Young Pnpla2 fl/fl and Pnpla2 AdipoQΔ/Δ mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs . Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS (Lin − cKIT + Sca-1 + ) and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. (E) Representative flow cytometry plots showing CD48 vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ) and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs . CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of (J) MPP3 and (K) MPP4 cells. Data shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=7-11 per group, males & females). p values determined using two-way ANOVA. NS, not significant; *p < 0.05. Data pooled from three independent experiments with a similar trend.

Article Snippet: The cells were stained with the following primary antibodies: Sca-1 FITC (#122505), Ly6C PerCP/Cy5.5 (#128012), CD115 PE (#135505), CD34 PE/Cy5 (#119311), F4/80 AF647 (#123122), CD11C BV605 (#117333), CD3 Biotin (#100243), CD49b Biotin (#103521), CD90.1 Biotin (#202510), all from Biolegend, CD135 PerCPeF710 (#46-1351-82), CD150 eF450 (#48-1502-82), MHCII eF506 (#69-5321-82) from Invitrogen, CD48 PE-Vio 770 (Miltenyi Biotec S. L, #130-102-363), c-KIT APC (BD Pharmingen, #553356), CD19 Biotin (eBioscience, #553784), for 25 min (4°C).

Techniques: Infection, Flow Cytometry

Journal: bioRxiv

Article Title: Age-dependent disease tolerance to SARS-CoV-2 infection

doi: 10.64898/2026.06.27.734955

Figure Lengend Snippet: ( A ) Young (3 months) and old (>20 months) mice were infected with MA-SARS-CoV-2 and BM was analyzed for early hematopoiesis at 5 dpi. ( B ) Representative flow cytometry plots showing c-KIT vs. Sca-1 gating on lineage-negative (Lin − ) cells to identify LKS (Lin − c-Kit + Sca-1 + ) hematopoietic stem and progenitor cells. Percentage and absolute numbers of ( C ) LKS cells and ( D ) c-KIT + (Lin − cKIT + Sca-1 − ) cells. ( E ) Representative flow cytometry plots showing CD48 vs. CD150 gating on LKS cells to identify long-term hematopoietic stem cells (LT-HSC: CD150 + CD48 − ), short-term HSC (ST-HSC: CD150 − CD48 − ), and multipotent progenitor 2 (MPP2: CD150 + CD48 + ). Percentage and absolute numbers of ( F ) LT-HSC, ( G ) ST-HSC and ( H ) MPP2 cells. ( I ) Representative flow cytometry plots showing CD135 vs. CD34 gating on MPP cells to identify MPP3 (CD135 − CD34 + ) and MPP4 (CD135 + CD34 + ). Percentage and absolute numbers of ( J ) MPP3 and ( K ) MPP4 cells. Data are shown as mean ± SD (C, D, F, G, H, J, K). Circles represent individual mice (n=6 per group, males). p values were determined using two-way ANOVA. NS, not significant; *p < 0.05, **p < 0.01; ***p < 0.001. Data pooled from two independent experiments with a similar trend.

Article Snippet: The cells were stained with the following primary antibodies: Sca-1 FITC (#122505), Ly6C PerCP/Cy5.5 (#128012), CD115 PE (#135505), CD34 PE/Cy5 (#119311), F4/80 AF647 (#123122), CD11C BV605 (#117333), CD3 Biotin (#100243), CD49b Biotin (#103521), CD90.1 Biotin (#202510), all from Biolegend, CD135 PerCPeF710 (#46-1351-82), CD150 eF450 (#48-1502-82), MHCII eF506 (#69-5321-82) from Invitrogen, CD48 PE-Vio 770 (Miltenyi Biotec S. L, #130-102-363), c-KIT APC (BD Pharmingen, #553356), CD19 Biotin (eBioscience, #553784), for 25 min (4°C).

Techniques: Infection, Flow Cytometry

Journal: The Febs Journal

Article Title: Butyrate suppresses mucosal inflammation in inflammatory bowel disease primarily through HDAC3 inhibition in monocytes and macrophages

doi: 10.1111/febs.70289

Figure Lengend Snippet: Butyrate reverts inflammatory mediators and enhances adherent‐invasive Escherichia coli (AIEC) phagocytosis in human THP‐1 macrophages. THP‐1 macrophages ( n = 3) were treated with butyrate in the presence or absence of lipopolysaccharide (LPS) + IFN‐γ (0.1 + 20 ng·mL −1 ) for 24 h. (A) CD40 and (B) CD80 geometric mean fluorescence intensity (gMFI) in THP‐1‐macrophages ( n = 3 or n = 5) were determined by flow cytometry. (C) Intracellular E. coli HS (commensal) and CD2‐a (AIEC) strains, normalized to control, were phagocytosed by THP‐1 macrophages treated with 2 m m butyrate (HS: n = 6; CD2‐a: n = 7). (D) mRNA levels of CD48 and MARCKS ( n = 3) were determined by RT‐qPCR. Statistical analysis: One‐way ANOVA with Bonferroni post‐test (A, B) and Wilcoxon matched‐pairs signed‐rank test (C, D). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Gene expression was analyzed using TaqMan Gene Expression Assays for SLC16A1 (Hs01560299_m1), HCAR2 (Hs02341584_s1), HDAC3 (Hs00187320_m1), IRF1 (Hs00971965_m1), TFF1 (Hs00907239_m1), DUOX2 (Hs00204187_m1), and LCN2 (Hs01008571_m1), MARCKS (Hs00158993_m1), and CD48 (Hs00381156_m1) (Applied Biosystems, Waltham, MA, USA) or TaqMan primers and probes from Eurogentec (Maastricht, The Netherlands; see Table ).

Techniques: Fluorescence, Flow Cytometry, Control, Quantitative RT-PCR