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3168008b 169tm cd25 2a3 fluidigm 3169003b 170er cd45ra hi100 fluidigm 3170010b 171yb cd195 np 6g4 fluidigm 3171017a  (fluidigm)


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    fluidigm 3168008b 169tm cd25 2a3 fluidigm 3169003b 170er cd45ra hi100 fluidigm 3170010b 171yb cd195 np 6g4 fluidigm 3171017a
    3168008b 169tm Cd25 2a3 Fluidigm 3169003b 170er Cd45ra Hi100 Fluidigm 3170010b 171yb Cd195 Np 6g4 Fluidigm 3171017a, supplied by fluidigm, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd195/us12521393-2842-249-253?v=fluidigm
    Average 92 stars, based on 4 article reviews
    3168008b 169tm cd25 2a3 fluidigm 3169003b 170er cd45ra hi100 fluidigm 3170010b 171yb cd195 np 6g4 fluidigm 3171017a - by Bioz Stars, 2026-07
    92/100 stars

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    (A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, <t>CCR5,</t> CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).
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    Editing efficiencies of SpCas9 and SpRYc across diverse PAM contexts at the <t>CCR5</t> target in 293T-CD4-CCR5 clone 19. ( A ) Sequences of sgRNAs for SpCas9 and SpRYc targeting the third exon of human CCR5 gene. The 110-nucleotide sequence of Homo sapiens (Hs) CCR5 exon 3 is shown. The sgRNA «sgR5-2» (PAM CGG) is not displayed, as it is located 117 nucleotides upstream of the depicted region. ( B ) CCR5 KO efficiency for different PAMs measured by flow cytometry. Data are presented as mean ± standard deviation (SD). Statistical significance was evaluated using one-way ANOVA with Dunnett’s multiple comparison test: ns, not significant; ****, p < 0.0001.
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    Editing efficiencies of SpCas9 and SpRYc across diverse PAM contexts at the <t>CCR5</t> target in 293T-CD4-CCR5 clone 19. ( A ) Sequences of sgRNAs for SpCas9 and SpRYc targeting the third exon of human CCR5 gene. The 110-nucleotide sequence of Homo sapiens (Hs) CCR5 exon 3 is shown. The sgRNA «sgR5-2» (PAM CGG) is not displayed, as it is located 117 nucleotides upstream of the depicted region. ( B ) CCR5 KO efficiency for different PAMs measured by flow cytometry. Data are presented as mean ± standard deviation (SD). Statistical significance was evaluated using one-way ANOVA with Dunnett’s multiple comparison test: ns, not significant; ****, p < 0.0001.
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    Image Search Results


    (A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, CCR5, CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).

    Journal: bioRxiv

    Article Title: The tuberculosis-associated microenvironment promotes HIV-1 persistence by impairing CD8+ T cell-mediated viral control

    doi: 10.1101/2025.10.14.682453

    Figure Lengend Snippet: (A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, CCR5, CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).

    Article Snippet: To characterize the cellular composition of TB-PE the following antibodies were included in a high-parameter flow cytometry panel: CD28-BUV395 (CD28.8, BD Biosciences), CD8-BUV496 (SK1, BD Biosciences), HLADQ-BUV563 (TU169, BD Biosciences), CD161-BUV615 (HP-3G10, BD Biosciences), CD154-BUV737 (TRAP1, BD Biosciences), CD4-BUV805 (OKT4, BD Biosciences), CCR7-BV421 ( -LI-A, BD Biosciences), CD39-BV480 (TU66, BD Biosciences), HLADR-BV605 (G46-6, BD Biosciences), PD1-BV711 (EH12.1, BD Biosciences), CD27-BV786 (LI27, BD Biosciences), CCR5-VioB515 (REA245, Miltenyi Biotec), CD3-NovaFluorB610 (UCHT1, Thermo Fisher Scientific), CD69-BB700 (FN50, BD Biosciences), TIGIT-PE (A15153G, BioLegend), CD103-PEVio615 (REA803, Miltenyi Biotec), CD127-PECY7 (R34.34, Thermo Fisher Scientific), and CD38-APCFire810 (HIT2, BioLegend).

    Techniques: Expressing, Derivative Assay

    Editing efficiencies of SpCas9 and SpRYc across diverse PAM contexts at the CCR5 target in 293T-CD4-CCR5 clone 19. ( A ) Sequences of sgRNAs for SpCas9 and SpRYc targeting the third exon of human CCR5 gene. The 110-nucleotide sequence of Homo sapiens (Hs) CCR5 exon 3 is shown. The sgRNA «sgR5-2» (PAM CGG) is not displayed, as it is located 117 nucleotides upstream of the depicted region. ( B ) CCR5 KO efficiency for different PAMs measured by flow cytometry. Data are presented as mean ± standard deviation (SD). Statistical significance was evaluated using one-way ANOVA with Dunnett’s multiple comparison test: ns, not significant; ****, p < 0.0001.

    Journal: International Journal of Molecular Sciences

    Article Title: The Chimeric Nuclease SpRYc Exhibits Highly Variable Performance Across Biological Systems

    doi: 10.3390/ijms27010488

    Figure Lengend Snippet: Editing efficiencies of SpCas9 and SpRYc across diverse PAM contexts at the CCR5 target in 293T-CD4-CCR5 clone 19. ( A ) Sequences of sgRNAs for SpCas9 and SpRYc targeting the third exon of human CCR5 gene. The 110-nucleotide sequence of Homo sapiens (Hs) CCR5 exon 3 is shown. The sgRNA «sgR5-2» (PAM CGG) is not displayed, as it is located 117 nucleotides upstream of the depicted region. ( B ) CCR5 KO efficiency for different PAMs measured by flow cytometry. Data are presented as mean ± standard deviation (SD). Statistical significance was evaluated using one-way ANOVA with Dunnett’s multiple comparison test: ns, not significant; ****, p < 0.0001.

    Article Snippet: For immunofluorescence staining, cells were washed once with PBS and incubated with Alexa 647-labelled antibodies against CCR5 (#E-AB-F1418M, Elabscience, USA) diluted in PBS at 4 °C for 30 min. Then, cells were washed twice with PBS and analyzed using a CytoFLEX S flow cytometer (Beckman-Coulter, USA).

    Techniques: Sequencing, Flow Cytometry, Standard Deviation, Comparison