cxcr2 (Bioss)
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cxcr2
Cxcr2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd182/CXCR2+CD182+Polyclonal+Antibody/pm41808159-160-17-19
Average 94 stars, based on 18 article reviews
Cxcr2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd182/CXCR2+CD182+Polyclonal+Antibody/pm41808159-160-17-19
Average 94 stars, based on 18 article reviews
cxcr2 - by Bioz Stars,
2026-11
94/100 stars
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Incubation:Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation Article Snippet: Polyvinylidene fluoride (PVDF) membranes (IPVH00010, Millipore) were pre-activated by immersing in methanol for 3 min to enhance protein binding. .. Proteins were then transferred onto the membranes using a constant current of 400 mA for 0.5 h. Following transfer, the membranes were incubated overnight at 4 °C with primary antibodies specific for CASP1 (WL03450, Wanleibio), CXCR2 (bs-1629R, Bioss), Article Title: Endothelial CXCR2 deficiency attenuates renal inflammation and glycocalyx shedding through NF-κB signaling in diabetic kidney disease Article Snippet: Equal amounts of protein were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, ISEQ00010). .. After blocking the membrane with 5% skim milk in TBST, the PVDF membrane was incubated with primary antibodies: p-IKKβ (1:1000, Cell Signaling Technology, 2697 T), IKKβ (1:1000, CST, 2678 T), p-IκBα (1:1000, CST, 2859), IκBα (1:1000, CST, 4812S), p-NF-κB p65 (1:1000, CST, 3033P), NF-κB p65 (1:1000, CST, 8242S), Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation. Article Snippet: .. Following transfer, the membranes were incubated overnight at 4°C with primary antibodies specific for CASP1 (WL03450, Wanleibio), Article Title: Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC-CXCL1-CXCR2 axis. Article Snippet: Equal amounts of protein were combined with 12% precast polyacrylamide gels (Bio-Rad Laboratories) and transferred onto nitrocellulose membranes (GE Healthcare). .. Nitrocellulose membranes with proteins were washed with 0.05% Tween-20 in Tris-buffered saline (TBST), blocked (ImmunoBlock; CTKN001, KAC) for 30 min at room temperature (20–25 °C) and then incubated with primary antibody (goat polyclonal anti-GAPDH antibody (1:1,000; sc20357, Santa Cruz Biotechnology), rabbit monoclonal anti-MYC antibody (1:1,000; ab32072, Abcam), anti-CXCR2 antibody (1:1,000; PA1029; Boster Biological Nature Cardiovascular Research Article https://doi.org/10.1038/s44161-025-00698-y Technology), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (1:1,000; 9101S, Cell Signaling Technology), p44/42 MAPK (Erk1/2) (137F5) antibody (1:1,000; 4695, Cell Signaling Technology) and Article Title: Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC–CXCL1–CXCR2 axis Article Snippet: Equal amounts of protein were combined with 12% precast polyacrylamide gels (Bio-Rad Laboratories) and transferred onto nitrocellulose membranes (GE Healthcare). .. Nitrocellulose membranes with proteins were washed with 0.05% Tween-20 in Tris-buffered saline (TBST), blocked (ImmunoBlock; CTKN001, KAC) for 30 min at room temperature (20–25 °C) and then incubated with primary antibody (goat polyclonal anti-GAPDH antibody (1:1,000; sc20357, Santa Cruz Biotechnology), rabbit monoclonal anti-MYC antibody (1:1,000; ab32072, Abcam), anti-CXCR2 antibody (1:1,000; PA1029; Boster Biological Technology), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (1:1,000; 9101S, Cell Signaling Technology), p44/42 MAPK (Erk1/2) (137F5) antibody (1:1,000; 4695, Cell Signaling Technology) and Blocking Assay:Article Title: Endothelial CXCR2 deficiency attenuates renal inflammation and glycocalyx shedding through NF-κB signaling in diabetic kidney disease Article Snippet: Equal amounts of protein were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, ISEQ00010). .. After blocking the membrane with 5% skim milk in TBST, the PVDF membrane was incubated with primary antibodies: p-IKKβ (1:1000, Cell Signaling Technology, 2697 T), IKKβ (1:1000, CST, 2678 T), p-IκBα (1:1000, CST, 2859), IκBα (1:1000, CST, 4812S), p-NF-κB p65 (1:1000, CST, 3033P), NF-κB p65 (1:1000, CST, 8242S), Membrane:Article Title: Endothelial CXCR2 deficiency attenuates renal inflammation and glycocalyx shedding through NF-κB signaling in diabetic kidney disease Article Snippet: Equal amounts of protein were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, ISEQ00010). .. After blocking the membrane with 5% skim milk in TBST, the PVDF membrane was incubated with primary antibodies: p-IKKβ (1:1000, Cell Signaling Technology, 2697 T), IKKβ (1:1000, CST, 2678 T), p-IκBα (1:1000, CST, 2859), IκBα (1:1000, CST, 4812S), p-NF-κB p65 (1:1000, CST, 3033P), NF-κB p65 (1:1000, CST, 8242S), other:Article Title: Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC-CXCL1-CXCR2 axis. Article Snippet: After washing, the membranes were incubated with a secondary horseradish peroxidase (HRP) antibody (donkey anti-goat IgG-HRP (1:2,000; sc-2020; Santa Cruz Biotechnology) and donkey anti-rabbit IgG-HRP (1:2,000; NA934V; Sigma-Aldrich)) for 1 h at room temperature (20– 25 °C). Saline:Article Title: Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC-CXCL1-CXCR2 axis. Article Snippet: Equal amounts of protein were combined with 12% precast polyacrylamide gels (Bio-Rad Laboratories) and transferred onto nitrocellulose membranes (GE Healthcare). .. Nitrocellulose membranes with proteins were washed with 0.05% Tween-20 in Tris-buffered saline (TBST), blocked (ImmunoBlock; CTKN001, KAC) for 30 min at room temperature (20–25 °C) and then incubated with primary antibody (goat polyclonal anti-GAPDH antibody (1:1,000; sc20357, Santa Cruz Biotechnology), rabbit monoclonal anti-MYC antibody (1:1,000; ab32072, Abcam), anti-CXCR2 antibody (1:1,000; PA1029; Boster Biological Nature Cardiovascular Research Article https://doi.org/10.1038/s44161-025-00698-y Technology), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (1:1,000; 9101S, Cell Signaling Technology), p44/42 MAPK (Erk1/2) (137F5) antibody (1:1,000; 4695, Cell Signaling Technology) and Article Title: Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC–CXCL1–CXCR2 axis Article Snippet: Equal amounts of protein were combined with 12% precast polyacrylamide gels (Bio-Rad Laboratories) and transferred onto nitrocellulose membranes (GE Healthcare). .. Nitrocellulose membranes with proteins were washed with 0.05% Tween-20 in Tris-buffered saline (TBST), blocked (ImmunoBlock; CTKN001, KAC) for 30 min at room temperature (20–25 °C) and then incubated with primary antibody (goat polyclonal anti-GAPDH antibody (1:1,000; sc20357, Santa Cruz Biotechnology), rabbit monoclonal anti-MYC antibody (1:1,000; ab32072, Abcam), anti-CXCR2 antibody (1:1,000; PA1029; Boster Biological Technology), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (1:1,000; 9101S, Cell Signaling Technology), p44/42 MAPK (Erk1/2) (137F5) antibody (1:1,000; 4695, Cell Signaling Technology) and |
