Journal: Frontiers in Medicine
Article Title: Reed-Sternberg cells express CD161 and lectin-like transcript 1 in Hodgkin lymphoma
doi: 10.3389/fmed.2026.1754727
Figure Lengend Snippet: (A–F) . CD161 expression patterns in Reed–Sternberg RS cells by immunohistochemistry (40x and 100x inset). (A) No detectable CD161 expression in RS cells, background immune cells are positive. (B) Low-level cytoplasmic only expression. (C) High-level cytoplasmic only expression. (D) High-level membranous-only expression. (E) Low-level dual cytoplasmic and membranous expression. (F) High-level dual cytoplasmic and membranous expression. The total expression level was calculated by multiplying the stain intensity score by the percentage of positive RS cells. Based on the median score, patients were classified into low expression (≤90; median), and high expression (>90; median) groups.
Article Snippet: The sections were incubated with primary antibodies specific to CD161 (anti-KLRB1/CD161 mAb clone 3D8F5, Proteintech Catalog No. 67537-1-Ig, RRID: AB_2882756 , 1:1,000), LLT1 (anti-CLEC2D mAb clone 4C7, Abnova, Catalog No. H00029121-M01, RRID: AB_463941 , 1:50), CD8 (anti-CD8 mAb clone 4B11, MyBioSource Catalog No. MBS215242, RRID: AB_10887243 , 1:50), and EBV (anti-EBV/LMP-1 mAb clone S20-D, MyBioSource Catalog No. MBS684063, 1:100).
Techniques: Expressing, Immunohistochemistry, Staining