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polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech polyclonal antibody
    Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccr2b/CCR2b-specific+Antibody/pmc12591211-373-17-19
    Average 93 stars, based on 8 article reviews
    polyclonal antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Combination therapy with bevacizumab and a CCR2 inhibitor for human ovarian cancer: An in vivo validation study.
    Article Snippet: .. Slides were incubated with primary antibodies against CD31 (1:1000 dilution, Cat# 11265- 1- AP, RRID:AB_2299349; Proteintech), CCR2A (1:200 dilution, Cat# 16153- 1- AP, RRID:AB_2262945; Proteintech), and CCR2B (1:300 dilution; Cat# 16154- 1- AP, RRID:AB_2878224; Proteintech). ..

    Article Title: Inducing Receptor Degradation as a Novel Approach to Target CC Chemokine Receptor 2 (CCR2)
    Article Snippet: Cells were then washed three times in PBS and simultaneously permeabilized and blocked for an hour using a modified blocking buffer (0.1% Triton ( v / v ), 1% BSA ( w / v ) (Sigma, A7030, Merck, Darmstadt, Germany)). .. Primary antibodies were diluted to 1:500 (CCR2b specific, 16154-1-AP, Proteintech, Manchester, UK; HiBiT Monoclonal antibody, N7200, Promega, Madison, WI, USA) and incubated at 4 °C overnight. .. Plates were washed three times in PBS and incubated with secondary antibody mix (Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488, A21206, 1:5000; Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647, A21235, 1:5000; Hoechst33342, H3570, 1:5000; Cell Mask Orange, C10045, 1:30,000 (Invitrogen, Carlsbad, CA, USA)) for 1–3 h and washed again three times in PBS.

    Article Title: Combination therapy with bevacizumab and CCR2 inhibitor for women with ovarian cancer: in vivo validation study
    Article Snippet: .. They were then incubated with primary antibodies against CCR2A (1:1 000 dilution, Proteintech Cat# 16153-1-AP, RRID:AB_2262945), and CCR2B (1:1 000 dilution; Proteintech Cat# 16154-1-AP, RRID:AB_2878224), p44/42 MAPK (Erk1/2, 1:1 000 dilution, Cell Signaling Technology Cat# 9102, RRID:AB_330744), phosphor-p44/42 MAPK (Erk1/2 Thr202/Tyr204, 1:1 000 dilution, Cell Signaling Technology Cat# 9101, RRID:AB_331646), and anti-actin (1:1 000 dilution, Millipore Cat# MAB1501, RRID:AB_2223041) in 5% milk/TBS-T or 5%BSA/TBS-T overnight at 4°C. ..

    Article Title: Combination therapy with bevacizumab and a CCR2 inhibitor for human ovarian cancer: An in vivo validation study
    Article Snippet: .. Slides were incubated with primary antibodies against CD31 (1:1000 dilution, Cat# 11265‐1‐AP, RRID:AB_2299349; Proteintech), CCR2A (1:200 dilution, Cat# 16153‐1‐AP, RRID:AB_2262945; Proteintech), and CCR2B (1:300 dilution; Cat# 16154‐1‐AP, RRID:AB_2878224; Proteintech). ..

    Saline:

    Article Title: Combination therapy with bevacizumab and a CCR2 inhibitor for human ovarian cancer: An in vivo validation study
    Article Snippet: SDS‐polyacrylamide gel electrophoresis (PAGE), which separated proteins using a 15% SDS sulfate‐polyacrylamide gel, was used to transfer the proteins to PVDF membranes (Millipore, Billerica, MA, USA). .. Membranes were blocked in 5% non‐fat dry milk in TBS‐T buffer (0.1% Tween‐20 in Tris‐buffered saline) for 1 h at room temperature and then exposed to primary antibodies against CCR2A (1:1000 dilution, Cat# 16153‐1‐AP, RRID:AB_2262945; Proteintech), and CCR2B (1:1000 dilution; Cat# 16154‐1‐AP, RRID:AB_2878224; Proteintech), p44/42 MAPK (Erk1/2, 1:1000 dilution, Cat# 9102, RRID:AB_330744; Cell Signaling Technology), phosphor‐p44/42 MAPK (Erk1/2 Thr202/Tyr204, 1:1000 dilution, Cat# 9101, RRID:AB_331646; Cell Signaling Technology), and anti‐actin (1:1000 dilution, Cat# MAB1501, RRID:AB_2223041; Millipore) in 5% milk/TBS‐T or 5%BSA/TBS‐T overnight at 4 °C. ..

    Article Title: Combination therapy with bevacizumab and a CCR2 inhibitor for human ovarian cancer: An in vivo validation study.
    Article Snippet: SDS- polyacrylamide gel electrophoresis (PAGE), which separated proteins using a 15% SDS sulfate- polyacrylamide gel, was used to transfer the proteins to PVDF membranes (Millipore, Billerica, MA, USA). .. Membranes were blocked in 5% non- fat dry milk in TBS- T buffer (0.1% Tween- 20 in Tris- buffered saline) for 1 h at room temperature and then exposed to primary antibodies against CCR2A (1:1000 dilution, Cat# 16153- 1- AP, RRID:AB_2262945; Proteintech), and CCR2B (1:1000 dilution; Cat# 16154- 1- AP, RRID:AB_2878224; Proteintech), p44/42 MAPK (Erk1/2, 1:1000 dilution, Cat# 9102, RRID:AB_330744; Cell Signaling Technology), phosphor- p44/42 MAPK (Erk1/2 Thr202/ Tyr204, 1:1000 dilution, Cat# 9101, RRID:AB_331646; Cell Signaling Technology), and anti- actin (1:1000 dilution, Cat# MAB1501, RRID:AB_2223041; Millipore) in 5% milk/TBS- T or 5%BSA/TBS- T overnight at 4 °C. ..



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    (A) Flow cytometry with surface staining for CCR2 on THP-1 CCR2 KO cells transduced with an empty vector (EV) or a vector encoding the wild-type (WT) or one of the various CCR2A and <t>CCR2B</t> variants. The results shown are representative of three independent experiments. (B and C) Intracellular calcium (Ca 2+ ) mobilization in transduced THP-1 CCR2 KO cells after stimulation with (B) CCL-2 or (C) ΔCCL-13 (n = 2–7 ± SD). (D) Migration of transduced THP-1 CCR2 KO toward CCL-2 or medium alone (n = 2–5 ± SD).
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    (A) Flow cytometry with surface staining for CCR2 on THP-1 CCR2 KO cells transduced with an empty vector (EV) or a vector encoding the wild-type (WT) or one of the various CCR2A and <t>CCR2B</t> variants. The results shown are representative of three independent experiments. (B and C) Intracellular calcium (Ca 2+ ) mobilization in transduced THP-1 CCR2 KO cells after stimulation with (B) CCL-2 or (C) ΔCCL-13 (n = 2–7 ± SD). (D) Migration of transduced THP-1 CCR2 KO toward CCL-2 or medium alone (n = 2–5 ± SD).
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    (A) Flow cytometry with surface staining for CCR2 on THP-1 CCR2 KO cells transduced with an empty vector (EV) or a vector encoding the wild-type (WT) or one of the various CCR2A and <t>CCR2B</t> variants. The results shown are representative of three independent experiments. (B and C) Intracellular calcium (Ca 2+ ) mobilization in transduced THP-1 CCR2 KO cells after stimulation with (B) CCL-2 or (C) ΔCCL-13 (n = 2–7 ± SD). (D) Migration of transduced THP-1 CCR2 KO toward CCL-2 or medium alone (n = 2–5 ± SD).
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    Image Search Results


    (A) Flow cytometry with surface staining for CCR2 on THP-1 CCR2 KO cells transduced with an empty vector (EV) or a vector encoding the wild-type (WT) or one of the various CCR2A and CCR2B variants. The results shown are representative of three independent experiments. (B and C) Intracellular calcium (Ca 2+ ) mobilization in transduced THP-1 CCR2 KO cells after stimulation with (B) CCL-2 or (C) ΔCCL-13 (n = 2–7 ± SD). (D) Migration of transduced THP-1 CCR2 KO toward CCL-2 or medium alone (n = 2–5 ± SD).

    Journal: Cell

    Article Title: Human inherited CCR2 deficiency underlies progressive polycystic lung disease

    doi: 10.1016/j.cell.2023.11.036

    Figure Lengend Snippet: (A) Flow cytometry with surface staining for CCR2 on THP-1 CCR2 KO cells transduced with an empty vector (EV) or a vector encoding the wild-type (WT) or one of the various CCR2A and CCR2B variants. The results shown are representative of three independent experiments. (B and C) Intracellular calcium (Ca 2+ ) mobilization in transduced THP-1 CCR2 KO cells after stimulation with (B) CCL-2 or (C) ΔCCL-13 (n = 2–7 ± SD). (D) Migration of transduced THP-1 CCR2 KO toward CCL-2 or medium alone (n = 2–5 ± SD).

    Article Snippet: CCR2B p.R327C F 5’-ACATCACCAAGTGCTTCTGCAAAC-3’ , Eurofins , N/A.

    Techniques: Flow Cytometry, Staining, Transduction, Plasmid Preparation, Migration

    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: Human inherited CCR2 deficiency underlies progressive polycystic lung disease

    doi: 10.1016/j.cell.2023.11.036

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: CCR2B p.R327C F 5’-ACATCACCAAGTGCTTCTGCAAAC-3’ , Eurofins , N/A.

    Techniques: Control, Virus, Clinical Proteomics, Recombinant, Staining, Imaging, Enzyme-linked Immunosorbent Assay, Isolation, Sequencing, Gene Expression, Subcloning, Software, Membrane

    (A) Flow cytometry with surface staining for CCR2 on THP-1 CCR2 KO cells transduced with an empty vector (EV) or a vector encoding the wild-type (WT) or one of the various CCR2A and CCR2B variants. The results shown are representative of three independent experiments. (B and C) Intracellular calcium (Ca 2+ ) mobilization in transduced THP-1 CCR2 KO cells after stimulation with (B) CCL-2 or (C) ΔCCL-13 (n = 2–7 ± SD). (D) Migration of transduced THP-1 CCR2 KO toward CCL-2 or medium alone (n = 2–5 ± SD).

    Journal: Cell

    Article Title: Human inherited CCR2 deficiency underlies progressive polycystic lung disease

    doi: 10.1016/j.cell.2023.11.036

    Figure Lengend Snippet: (A) Flow cytometry with surface staining for CCR2 on THP-1 CCR2 KO cells transduced with an empty vector (EV) or a vector encoding the wild-type (WT) or one of the various CCR2A and CCR2B variants. The results shown are representative of three independent experiments. (B and C) Intracellular calcium (Ca 2+ ) mobilization in transduced THP-1 CCR2 KO cells after stimulation with (B) CCL-2 or (C) ΔCCL-13 (n = 2–7 ± SD). (D) Migration of transduced THP-1 CCR2 KO toward CCL-2 or medium alone (n = 2–5 ± SD).

    Article Snippet: CCR2B pTRIP subcloning R 5’- AGGTCTCGAGGTCGACTTATAAACCAGCCGAGACTTCC-3’ , Eurofins , N/A.

    Techniques: Flow Cytometry, Staining, Transduction, Plasmid Preparation, Migration

    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: Human inherited CCR2 deficiency underlies progressive polycystic lung disease

    doi: 10.1016/j.cell.2023.11.036

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: CCR2B pTRIP subcloning R 5’- AGGTCTCGAGGTCGACTTATAAACCAGCCGAGACTTCC-3’ , Eurofins , N/A.

    Techniques: Control, Virus, Clinical Proteomics, Recombinant, Staining, Imaging, Enzyme-linked Immunosorbent Assay, Isolation, Sequencing, Gene Expression, Subcloning, Software, Membrane