Journal: NPJ Parkinson's Disease
Article Title: Microglia depletion reduces neurodegeneration and remodels extracellular matrix in a mouse Parkinson’s disease model triggered by α-synuclein overexpression
doi: 10.1038/s41531-024-00846-4
Figure Lengend Snippet: a Volcano plot analysis of RNA-seq data from PD model animals with and without PLX5622 challenge. b GO analysis of RNA-seq data showing the signaling pathways of most significantly up-regulated genes in hSYN + PLX5622 / hSYN + vehicle. c Representation of protein-protein network of the top 32 up-regulated genes in hSYN + PLX5622 / hSYN + vehicle using STRING database. d Quantification of gene expression related to extracellular matrix with RNA-seq data and quantitative real-time PCR. e Western blot analysis of CCN2 and CCN3 protein in the ventral midbrain. f , g Quantification of relative CCN2 ( f ) and CCN3 ( g ) expression to GAPDH. * p < 0.05, ** p < 0.01. Data expressed as mean ± SEM ( n = 3/group).
Article Snippet: The membranes were blocked with 5% DifcoTM Skim Milk (BD, Cat#:232100, Lot#: 6342932) and incubated at 4 °C overnight with primary antibodies: GAPDH Mouse Monoclonal Antibody (Abways, Cat#: AB0038, Lot#: F007201, 1:3000), Mouse NOV/CCN3 Antibody (R&D system, Cat#: AF1976, Lot#: K007405P, 1:500), Anti-CTGF Polyclonal Antibody (Solarbio, Cat#: K007405P, Lot#: 20230217, 1:500), followed by HRP-conjugated secondary antibodies: HRP-labeled Donkey Anti-Goat IgG (H + L) (Beyotime, Cat#: A0181, 1:1000), HRP-labeled Goat Anti-Mouse IgG (H + L) (Beyotime, Cat#: A0216, Lot#: 062317171102, 1:1000), HRP-labeled Goat Anti-Rabbit IgG (H + L) (Beyotime, Cat#: A0208, Lot#: 060017171031, 1:1000).
Techniques: RNA Sequencing Assay, Expressing, Real-time Polymerase Chain Reaction, Western Blot
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