colon cell line ccd841  (ATCC)

Journal: iScience

Article Title: CCL19-armed recombinant influenza virus inhibited colorectal cancer growth by remodeling tumor microenvironment

doi: 10.1016/j.isci.2025.114127

Figure Lengend Snippet: The replication and tumor cell-killing activity of r PR8-CCL19 in vitro (A) The proliferation dynamics of r PR8-CCL19 in various CRC cell lines (CT26, HT29, HCT116, SW620, and Lovo) and the normal colonic mucosal epithelial cell line CCD841, as determined by the HA titter measurement. Data were analyzed with descriptive statistical method and represented as mean ± SEM. n represents technical replicates, n = 3. (B) Cell killing study performed through xCELLigence RTCA representing individual cell impedance measurements of cells in real time in response to the treatment with r PR8-CCL19, wt PR8, and r CCL19, respectively. The control group (CON) with non-treatment was also applied. (C) The flow cytometric analysis of apoptosis in HT29 cells infected with r PR8-CCL19 and wt PR8 at 24, 48, and 72 h post-infection, respectively. The CON with non-treatment was also applied. (D) Quantification of HT29 cells apoptotic ratio. r PR8-CCL19 induced more than 78% cell apoptosis at 24 h post-infection. Data were analyzed with unpaired two-sided Student’s t test and represented as mean ± SEM. n represents technical replicates, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. (E) Detection of changes in mitochondrial membrane potential (MMP) in HT29 cells at 24 h post-infection with r PR8-CCL19 by flow cytometry, compared with wt PR8 and CON. (F) Quantification of MMP in HT29 cell. MMP decreased significantly after r PR8-CCL19 treatment. Data were analyzed with unpaired two-sided Student’s t test and represented as mean ± SEM. n represents technical replicates, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. (G) The expressions of PARP, caspase-3, caspase-9, caspase-8, and their corresponding cleavers in HT29 cells infected with r PR8-CCL19 or wt PR8 were detected by western blotting, respectively. (H) The expressions of BAX, Bcl-2, cyto-cyc (cytochrome in cytoplasm), and mito-cyc (cytochrome in mitochondria) in HT29 cells infected with r PR8-CCL19 or wt PR8 were detected by western blotting, respectively.

Article Snippet: CCD841 , ATCC , CRL-1790.

Techniques: Activity Assay, In Vitro, Control, Infection, Membrane, Flow Cytometry, Western Blot

Journal: Discover Oncology

Article Title: LINC01063 promotes colon cancer progression via the miR-134-5p/KRAS axis and serves as a prognostic biomarker

doi: 10.1007/s12672-025-03961-7

Figure Lengend Snippet: Relative expression levels of LINC01063 and miR-134-5p in different colon cell lines. A Relative expression levels of LINC01063. B Relative expression levels of miR-134-5p. Cell lines included normal colon epithelial cell line CCD841 and colon cancer cell lines SW480, RKO, HCT116, and COLO320. Compared with the CCD841 cell line, **** p < 0.0001

Article Snippet: Colon cancer cells (SW480, RKO, HCT116, and COLO320 cells) and normal control cells (CCD841) were obtained from the American Type Culture Conservation Center (ATCC) and were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) routinely at 37 °C with 5% CO2.

Techniques: Expressing