cay10499  (MedChemExpress)

Journal: iScience

Article Title: HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells

doi: 10.1016/j.isci.2025.112441

Figure Lengend Snippet: Yield of infectious HSV-1 and viral protein synthesis in BMDCs treated with inhibitors of lipid-metabolism-related enzymes Plaque-forming units (PFUs) and viral protein expression analyses (western blot) of HSV-1 late viral proteins (glycoprotein B, glycoprotein D, and VP16) in BMDCs treated for 3 h with the (A) DGAT1 inhibitor A-922500 (10 μM) (B) DGAT2 inhibitor PF-06424439 (10 μM), (C) ACAT inhibitor avasimibe (10 μM), (D) ACS inhibitor TOFA (10 μM), (E) ACS inhibitor triacsin C (10 μM), (F) lipase inhibitor CAY10499 (10 μM), (G) FATP inhibitor lipofermata (10 μM), or (H) CD36 inhibitor sulfosuccinimidyl oleate (SSO, 50 μM) and then infected with HSV-1 strain F at MOI 3 for 12 hpi or 18 hpi. (−) corresponds to vehicle treatment (DMSO) and (+) to treatment with the inhibitor. C + corresponds to Vero-HSV-1-infected cells (positive control). The analyses shown are means ± SEM of three independent assays. The statistical analyses were performed using two-way ANOVA and Bonferroni multiple comparisons test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns: no statistical significance). See also Figure S5 .

Article Snippet: CAY10499 , Cayman Chemical , Cat#10007875; CAS No. 359714-55-9.

Techniques: Expressing, Western Blot, Infection, Positive Control

Journal: iScience

Article Title: HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells

doi: 10.1016/j.isci.2025.112441

Figure Lengend Snippet: Inhibition of cholesterol ester synthesis (ACAT) or fatty acid uptake by fatty acid transport protein (FATP) recovers the viability of HSV-1-infected BMDCs Flow cytometry analyses of BMDC viability (determined as CD11c + /Zombie − cells) treated with (A) DGAT1 inhibitor A-922500 (10 μM), (B) DGAT2 inhibitor PF-06424439 (10 μM), (C) ACAT inhibitor avasimibe (10 μM), (D) ACS inhibitor TOFA (10 μM), (E) ACS inhibitor triacsin C (10 μM), (F) lipase inhibitor CAY10499 (10 μM), (G) FATP inhibitor lipofermata (10 μM), or (H) CD36 inhibitor sulfosuccinimidyl oleate (SSO, 50 μM) for 3 h, infected with HSV-1 strain F at MOI 3 for 1 h, and then further treated with the inhibitors for 17 h during infection. Vehicle treatment corresponds to the solvent used to dissolve the inhibitors (DMSO). The analyses shown are means ± SEM of three independent assays. The statistical analyses were performed using two-way ANOVA and Bonferroni multiple comparisons test (∗ p < 0.05, ∗∗ p < 0.01, ns: no statistical significance). See also Figures S6 and .

Article Snippet: CAY10499 , Cayman Chemical , Cat#10007875; CAS No. 359714-55-9.

Techniques: Inhibition, Infection, Flow Cytometry, Solvent

Journal: Nature Communications

Article Title: Sortilin-mediated translocation of mitochondrial ACSL1 impairs adipocyte thermogenesis and energy expenditure in male mice

doi: 10.1038/s41467-024-52218-4

Figure Lengend Snippet: a Workflow of shcon and shSort1 adipocytes for mRNA-seq. n = 3 per group. b Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of global activated pathway in shSort1 adipocytes. c Immunoblots (proteins indicated) in shcon and shSort1 adipocytes. The right panel showed the quantification normalized to α-Tubulin, AMPK, p38 MAPK or HSL. n = 3 per group. p/T AMPK: phosphorylated AMPK/Total AMPK; p/T p38 MAPK: phosphorylated p38 MAPK/Total p38 MAPK; p/T HSL: phosphorylated HSL/Total HSL. d Immunoblots (proteins indicated) in ingWAT of Sort1 fl/fl and Sort1 adipo KO mice. The right panel showed the quantification normalized to α-Tubulin, AMPK, p-38 MAPK or HSL. n = 6 per group. e Immunoblots (proteins indicated) in shcon and shSort1 adipocytes incubated with or without AMPK inhibitor CC (10 μM) for 2 h. The lower panel showed the quantification normalized to α-Tubulin or AMPK. n = 4 per group. f Immunoblots (proteins indicated) of shcon and shSort1 adipocytes that were reverse-transfected with plasmid expressing AMPK-DN or control mock for 24 h. The lower panel showed the quantification normalized to α-Tubulin or AMPK. n = 4 per group. g , h Immunoblots (proteins indicated) in shcon and shSort1 adipocytes incubated with or without p-38 MAPK inhibitor SB203580 (10 μM) ( g ) or SB202190 (5 μM) ( h ) for 2 h. The lower or right panels showed the quantification normalized to α-Tubulin or p-38 MAPK. n = 4 per group. i Immunoblots (proteins indicated) in shcon and shSort1 adipocytes incubated with or without lipase inhibitor (Atglistatin, ATGL inhibitor 10 μM and CAY10499, HSL inhibitor 20 μM) ( h ) for 2 h. The right panel showed the quantification normalized to α-Tubulin or HSL. n = 4 per group. j The AMP/ATP and ADP/ATP ratio in ingWAT of 8-week-old Sort1 fl/fl and Sort1 adipo KO mice fed chow diet. n = 7 for Sort1 fl/fl group, n = 9 for Sort1 adipo KO group. k Metabolites levels of glycolysis and tricarboxylic acid cycle (TCA) in ingWAT of 8-week-old Sort1 fl/fl and Sort1 adipo KO mice fed chow diet. n = 7 for Sort1 fl/fl group, n = 9 for Sort1 adipo KO group. Statistical differences were determined by two-tailed Student’s t-test; Data were presented as mean ± SEMs. Source data and uncropped blots are available as a Source Data file.

Article Snippet: For the inhibitors’ treatment, cells were pre-incubated with AMPK inhibitor compound C (CC, 10 μM, no. HY-13418A, MCE); the lipase inhibitors, including ATGL inhibitor Atglistatin (10 μM, no. HY-15859, MCE) and HSL inhibitor CAY10499 (20 μM, no. HY-119283, MCE); p38 MAPK inhibitors SB203580 (10 μM, no. HY-10256, MCE) and SB202190 (5 μM, no. HY-10295, MCE); lysosomal inhibitors NH4Cl (250 μM, no. HY-Y1269, MCE) and chloroquine (20 μM, no. HY-17589A, MCE); autophagy inhibitor 3-MA (20 μM, no. HY-19312, MCE) and proteasome inhibitor MG132 (20 μM, no. HY-13259, MCE).

Techniques: Western Blot, Incubation, Transfection, Plasmid Preparation, Expressing, Control, Two Tailed Test

Journal: The Journal of Reproduction and Development

Article Title: Lipid droplets synthesized during luteinization are degraded after pregnancy

doi: 10.1262/jrd.2023-095

Figure Lengend Snippet: Degradation of LDs by lipases in luteinized granulosa cells. (A) Representative images of luteinized granulosa cells isolated from mCherry-HPos mice (2 h after hCG treatment), which were cultured in the presence of DMSO (control), Atglistatin, or CAY10499 together with Lipi-Green for 1 day and observed with a spinning disk confocal microscope. Zoom panels show enlarged images of the boxed area. Scale bar, 50 μm. (B) Quantification of average LD size and number per cell in luteinized granulosa cells cultured as in (A). Data are presented as the mean ± SEM; Student’s t -test.

Article Snippet: To block lipase activity, luteinized granulosa cells were cultured overnight in the presence of 40 μM Atglistatin (SML1075; Sigma-Aldrich, St. Louis, MO, USA) or 20 μM CAY10499 (10007875; Cayman Chemical, Ann Arbor, MI, USA) dissolved in DMSO together with 0.1 μM Lipi-Green (LD02; Dojindo Laboratories, Kumamoto, Japan).

Techniques: Isolation, Cell Culture, Control, Microscopy