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anti casr antibody  (Proteintech)


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    Structured Review

    Proteintech anti casr antibody
    Anti Casr Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/casr/Calcium+Sensing+Receptor+Antibody/pm41594470-139-51-54
    Average 93 stars, based on 33 article reviews
    anti casr antibody - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Saline:

    Article Title: Renal tubular injury induced by glyphosate combined with hard water: the role of cytosolic phospholipase A2
    Article Snippet: .. Subsequently, slides were washed three times in phosphate-buffered saline (PBS) and blocked with 5% goat serum for 30 minutes at room temperature, followed by overnight incubation at 4 °C with primary antibodies against the CaSR (1:100, Proteintech Group). .. Slides were then incubated with Alexa Fluor 488-conjugated goat anti-rabbit antibody (1:1,000, CST) for 1 hour at room temperature in the dark.

    Article Title: miR-135 protects against atrial fibrillation by suppressing intracellular calcium-mediated NLRP3 inflammasome activation.
    Article Snippet: Atrial fibrillation (AF), one of the most common types of arrhythmias, is associated with high morbidity and mortality, seriously endangering human health.. Inflammation is closely associated with AF development.. Activation of the nucleotidebinding domain-like receptor protein 3 (NLRP3) inflammasome in cardiomyocytes has been shown to promote AF progression.

    Incubation:

    Article Title: Renal tubular injury induced by glyphosate combined with hard water: the role of cytosolic phospholipase A2
    Article Snippet: .. Subsequently, slides were washed three times in phosphate-buffered saline (PBS) and blocked with 5% goat serum for 30 minutes at room temperature, followed by overnight incubation at 4 °C with primary antibodies against the CaSR (1:100, Proteintech Group). .. Slides were then incubated with Alexa Fluor 488-conjugated goat anti-rabbit antibody (1:1,000, CST) for 1 hour at room temperature in the dark.

    Article Title: Inhibition of calcium-sensing receptor by its antagonist protects dopaminergic neurons from MPTP/MPP + -induced neurotoxicity via regulating mitochondrial function, autophagy, and apoptosis in vivo and in vitro.
    Article Snippet: Parkinson’s disease (PD) is characterized by progressive degeneration of dopaminergic neurons.. The role of calcium-sensing receptor (CaSR) in the pathogenesis of PD remains poorly understood.. We employed the CaSR antagonist NPS-2143 in both in vivo and in vitro experiments to investigate the therapeutic potential of CaSR modulation.

    Article Title: miR-429 and miR-424-5p inhibit cell proliferation and Ca 2+ influx by downregulating CaSR in pulmonary artery smooth muscle cells.
    Article Snippet: .. Membranes were then incubated with primary antibodies against GAPDH 106 (Cat.No.10494-1-AP, ProteinTech, Wuhan, China), β-actin (Cat.No.20536-1-AP, ProteinTech, 107 Wuhan, China) or CaSR (Cat.No.19125-1-AP, ProteinTech, Wuhan, China) at a dilution of 108 1:5,000 in 3% skim milk diluted in TTBS for 1h at room temperature. ..

    Article Title: Comparison of the Construction Methods of Parathyroid Organoids and Functional Influence of Calcium on Organoids.
    Article Snippet: .. Briefly, paraffinembedded organoid sections were initially processed following the H&E staining protocol up to the hydration step, where the sections were immersed in double- steaming water for 5 min. Antigen retrieval was performed using citrate buffer (10 mM sodium citrate with 0.05% Tween- 20, pH 6.0), which was preheated by microwave until boiling, and sections were incubated in the buffer at 95 °C for 30 min. After cooling and PBS rinsing (2 min), sections were blocked with 3% BSA at room temperature for 1 h. Primary antibodies used included PTH (Proteintech, #10817- 1- AP), GCM2 (Proteintech, #21088- 1- AP), CHGA (Proteintech, #10529- 1- AP), and CaSR (Proteintech, #19125- 1- AP), applied overnight at 4 °C in a humidified chamber according to the experimental group. ..

    Article Title: miR-135 protects against atrial fibrillation by suppressing intracellular calcium-mediated NLRP3 inflammasome activation.
    Article Snippet: Atrial fibrillation (AF), one of the most common types of arrhythmias, is associated with high morbidity and mortality, seriously endangering human health.. Inflammation is closely associated with AF development.. Activation of the nucleotidebinding domain-like receptor protein 3 (NLRP3) inflammasome in cardiomyocytes has been shown to promote AF progression.

    Control:

    Article Title: L-tetrahydropalmatine attenuates methamphetamine-induced pulmonary vascular remodeling via CaSR-BMP2/mTOR signaling.
    Article Snippet: The World Symposium on Pulmonary Hypertension (WSPH) raised a new classification, “Drugand toxininduced pulmonary arterial hypertension (PAH)”, in which methamphetamine (MA) is recognized as an important contributor.. L-tetrahydropalmatine (L-THP), an alkaloid with analgesic and sedative properties, has demonstrated vascular protective effects, but its mechanism against MA-induced pulmonary injury remains unclear.. The calcium-sensing receptor (CaSR) regulates multiple cellular processes, including proliferation, differentiation, migration, adhesion, and apoptosis, and may participate in MA-related pulmonary vascular remodeling.

    Staining:

    Article Title: Comparison of the Construction Methods of Parathyroid Organoids and Functional Influence of Calcium on Organoids.
    Article Snippet: .. Briefly, paraffinembedded organoid sections were initially processed following the H&E staining protocol up to the hydration step, where the sections were immersed in double- steaming water for 5 min. Antigen retrieval was performed using citrate buffer (10 mM sodium citrate with 0.05% Tween- 20, pH 6.0), which was preheated by microwave until boiling, and sections were incubated in the buffer at 95 °C for 30 min. After cooling and PBS rinsing (2 min), sections were blocked with 3% BSA at room temperature for 1 h. Primary antibodies used included PTH (Proteintech, #10817- 1- AP), GCM2 (Proteintech, #21088- 1- AP), CHGA (Proteintech, #10529- 1- AP), and CaSR (Proteintech, #19125- 1- AP), applied overnight at 4 °C in a humidified chamber according to the experimental group. ..



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    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    Image Search Results


    Calcium oxalate crystals upregulate CaSR and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: Calcium oxalate crystals upregulate CaSR and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Expressing, In Vivo, In Vitro, Staining, Light Microscopy, Western Blot, Quantitative RT-PCR, Immunohistochemical staining, Standard Deviation, Control

    CaSR regulates SLC26A6 expression via the transcription factor FOXO4. (A) Western blotting detection of p-FOXO4 (Thr451) and SLC26A6 protein expression levels in NRK-52E cells treated with CaSR agonist (R568, 30 μM) or inhibitor (NPS-2143, 10 μM) in the presence of COM crystals. (B) Western blotting detection of SLC26A6 protein expression levels in NRK-52E cells treated with FOXO4 agonist (TNF-α, 20 ng/mL) or inhibitor (JY-2, 30 μM) in the presence of COM crystals. (C) Dual-luciferase reporter gene assay detecting the effect of FOXO4 on SLC26A6 promoter activity. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. COM group; #p < 0.05, ##p < 0.01 vs. specified group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: CaSR regulates SLC26A6 expression via the transcription factor FOXO4. (A) Western blotting detection of p-FOXO4 (Thr451) and SLC26A6 protein expression levels in NRK-52E cells treated with CaSR agonist (R568, 30 μM) or inhibitor (NPS-2143, 10 μM) in the presence of COM crystals. (B) Western blotting detection of SLC26A6 protein expression levels in NRK-52E cells treated with FOXO4 agonist (TNF-α, 20 ng/mL) or inhibitor (JY-2, 30 μM) in the presence of COM crystals. (C) Dual-luciferase reporter gene assay detecting the effect of FOXO4 on SLC26A6 promoter activity. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. COM group; #p < 0.05, ##p < 0.01 vs. specified group.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Expressing, Western Blot, Luciferase, Reporter Gene Assay, Activity Assay, Standard Deviation

    CaSR promotes FOXO4 phosphorylation via the PKA signaling pathway. (A) Western blotting detection of p-PKA substrate and p-FOXO4 (Thr451) protein expression levels in NRK-52E cells treated with CaSR agonist (R568, 30 μM) or inhibitor (NPS-2143, 10 μM) in the presence of COM crystals. (B) Western blotting detection of p-FOXO4 (Thr451) and SLC26A6 protein expression levels in NRK-52E cells treated with PKA agonist (8-Bromo-cAMP, 30 μM) or inhibitor (H-89 2HCl, 30 μM) in the presence of COM crystals. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. COM group; #p < 0.05, ##p < 0.01 vs. specified group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: CaSR promotes FOXO4 phosphorylation via the PKA signaling pathway. (A) Western blotting detection of p-PKA substrate and p-FOXO4 (Thr451) protein expression levels in NRK-52E cells treated with CaSR agonist (R568, 30 μM) or inhibitor (NPS-2143, 10 μM) in the presence of COM crystals. (B) Western blotting detection of p-FOXO4 (Thr451) and SLC26A6 protein expression levels in NRK-52E cells treated with PKA agonist (8-Bromo-cAMP, 30 μM) or inhibitor (H-89 2HCl, 30 μM) in the presence of COM crystals. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. COM group; #p < 0.05, ##p < 0.01 vs. specified group.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Phospho-proteomics, Western Blot, Expressing, Standard Deviation

    Crystal deposition and tissue damage in the kidneys of rats from each group (A) Hematoxylin and eosin (HE) staining, polarized light microscopy, and Pizzolato’s calcium salt staining of kidney tissue from rats in the EG, CaSR-a, and CaSR-i groups. HE staining reveals tubular architecture and the extent of damage; polarized light microscopy reveals the birefringence of calcium oxalate crystals; Pizzolato’s staining stains calcium oxalate crystals black. (B) Hematoxylin and eosin (HE) staining, polarized light microscopy, and Pizzolato’s calcium salt staining of renal tissues from rats in the EG, PKA-i, and FOXO4-i groups. HE staining reveals the extent of tubular damage in each group; polarized light microscopy and calcium salt staining jointly confirm differences in crystal deposition among the groups.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: Crystal deposition and tissue damage in the kidneys of rats from each group (A) Hematoxylin and eosin (HE) staining, polarized light microscopy, and Pizzolato’s calcium salt staining of kidney tissue from rats in the EG, CaSR-a, and CaSR-i groups. HE staining reveals tubular architecture and the extent of damage; polarized light microscopy reveals the birefringence of calcium oxalate crystals; Pizzolato’s staining stains calcium oxalate crystals black. (B) Hematoxylin and eosin (HE) staining, polarized light microscopy, and Pizzolato’s calcium salt staining of renal tissues from rats in the EG, PKA-i, and FOXO4-i groups. HE staining reveals the extent of tubular damage in each group; polarized light microscopy and calcium salt staining jointly confirm differences in crystal deposition among the groups.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Staining, Light Microscopy

    Effects of intervening in the CaSR-PKA-FOXO4 signaling axis on urinary biochemical indicators and renal protein expression in rats. (A) Western blotting detection of p-PKA substrate, p-FOXO4 (Thr451), and SLC26A6 protein expression levels in renal tissues of each group of rats. (B) Concentrations of calcium ions and oxalate in 24-h urine of each group of rats. (C) Quantitative analysis of SLC26A6 protein expression from (A) . Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. E.G., group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: Effects of intervening in the CaSR-PKA-FOXO4 signaling axis on urinary biochemical indicators and renal protein expression in rats. (A) Western blotting detection of p-PKA substrate, p-FOXO4 (Thr451), and SLC26A6 protein expression levels in renal tissues of each group of rats. (B) Concentrations of calcium ions and oxalate in 24-h urine of each group of rats. (C) Quantitative analysis of SLC26A6 protein expression from (A) . Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. E.G., group.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Expressing, Western Blot, Standard Deviation

    Schematic diagram of the mechanism by which CaSR synergistically promotes calcium oxalate stone formation through dual signaling pathways. Under stimulation by calcium oxalate crystals (COM), the calcium-sensing receptor (CaSR) on the membrane of renal tubular epithelial cells is activated. The activated CaSR activates protein kinase A (PKA) via G proteins. PKA then functions through two parallel pathways: Phosphorylates Signal Transducer and Activator of Transcription 3 (STAT3), causing its nuclear translocation and upregulation of the tight junction protein Claudin-14 expression. Claudin-14 inhibits paracellular calcium reabsorption, leading to hypercalciuria; Phosphorylates Forkhead box protein O4 (FOXO4), causing its nuclear translocation and binding to the SLC26A6 gene promoter, upregulating its expression and promoting oxalate secretion into the lumen, leading to hyperoxaluria. The increased excretion of urinary calcium and oxalate collectively exacerbates the supersaturation of calcium oxalate in urine, ultimately promoting kidney stone formation.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: Schematic diagram of the mechanism by which CaSR synergistically promotes calcium oxalate stone formation through dual signaling pathways. Under stimulation by calcium oxalate crystals (COM), the calcium-sensing receptor (CaSR) on the membrane of renal tubular epithelial cells is activated. The activated CaSR activates protein kinase A (PKA) via G proteins. PKA then functions through two parallel pathways: Phosphorylates Signal Transducer and Activator of Transcription 3 (STAT3), causing its nuclear translocation and upregulation of the tight junction protein Claudin-14 expression. Claudin-14 inhibits paracellular calcium reabsorption, leading to hypercalciuria; Phosphorylates Forkhead box protein O4 (FOXO4), causing its nuclear translocation and binding to the SLC26A6 gene promoter, upregulating its expression and promoting oxalate secretion into the lumen, leading to hyperoxaluria. The increased excretion of urinary calcium and oxalate collectively exacerbates the supersaturation of calcium oxalate in urine, ultimately promoting kidney stone formation.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Protein-Protein interactions, Membrane, Translocation Assay, Expressing, Binding Assay