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anti caspase8  (Proteintech)


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    Structured Review

    Proteintech anti caspase8
    Anti Caspase8, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase8/Caspase+8%2Fp43%2Fp18+Antibody/pm41411841-309-48-51
    Average 96 stars, based on 395 article reviews
    anti caspase8 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Ubiquitin Proteomics:

    Article Title: Tetrahydrocurcumin targets TRIP13 inhibiting the interaction of TRIP13/USP7/c-FLIP to mediate c-FLIP ubiquitination in triple-negative breast cancer
    Article Snippet: .. Primary antibodies, including PARP (#27246, CST, MA, USA), Caspase3 (#9662, CST, MA, USA), Cleave-caspase3 (#9664, CST, MA, USA), Caspase8 (66093-1-Ig, Proteintech, MA, USA), c-FLIP (#56343, CST, MA, USA), Ubiquitin (#3936, CST, MA, USA /10201-2-AP, Proteintech, MA, USA), IgG (30000-0-AP, Proteintech, MA, USA), TRIP13 (67759-1-Ig/19602-1-AP, Proteintech, MA, USA), USP7 (66514-1-Ig, Proteintech, MA, USA) and β-actin (TA-09, ZSGB-BIO, Beijing, China) were diluted at 1:500 or 1:2000. .. TNBC cells were transfected with CFLAR/TRIP13 small interfering RNA (siRNA) synthesized by GenePharma (Shanghai, China), or transiently transfected with CFLAR overexpression plasmid (Genechem, Shanghai, China) using Lipofectamine 2000 (Invitrogen, USA).

    Blocking Assay:

    Article Title: Cecropin AD ameliorates pneumonia and intestinal injury in mice with mycoplasma pneumoniae by mediating gut microbiota
    Article Snippet: To block the membrane, place the membrane in skim milk (BD Life Sciences, Bridgeport, NJ, USA) dissolved in TBST (50 mmol/L of Tris, 0.1% Tween20, 150 mmol/L of NaCl, and pH 7.6), after blocking for 2 h at 25 °C. .. After blocking, the membranes were incubated with the primary antibody at 4 °C overnight, followed by incubation with a horseradish peroxidase-conjugated secondary antibody at 25 °C for 3 h. The primary antibodies used were as follows: Occludin (Proteintech, 27260-1-AP, Wuhan, China), Claudin-1 (Santacruz, 3523011327, CA, USA), ZO-1 (Proteintech, 21773-1-AP, Wuhan, China), TLR-4 ( Proteintech, 66350-1-IG, Wuhan, China), Myd88 ( Proteintech, 23230-1-AP, Wuhan, China), NF-κB p65 (Proteintech, 10745-1-AP, Wuhan, China), Phospho-NF-κB p65 (Proteintech, 82335-1-RR, Wuhan, China), IkB (Proteintech, 10268-1-AP, Wuhan, China), Phospho-IkB (Proteintech, 82349-1-RR, Wuhan, China), Caspase8 (Santacruz,,#5800803, CA, USA), Caspase3 (Affinity, 00125840, Jiangsu, China), FADD (Affinity, #237743, Jiangsu, China), Cyt-c(Affinity, #73C2522, Jiangsu, China), BAX (Proteintech, 50599-2-Ig, Wuhan, China), Bcl-2 (Proteintech, 82469-6-RR, Wuhan, China), β-actin (Santacruz, #E2422, CA, USA). .. The secondary antibodies used were as follows: HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (Proteintech, SA00013-1, Wuhan, China)、 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (Proteintech, SA00013-2, Wuhan, China).

    Incubation:

    Article Title: Cecropin AD ameliorates pneumonia and intestinal injury in mice with mycoplasma pneumoniae by mediating gut microbiota
    Article Snippet: To block the membrane, place the membrane in skim milk (BD Life Sciences, Bridgeport, NJ, USA) dissolved in TBST (50 mmol/L of Tris, 0.1% Tween20, 150 mmol/L of NaCl, and pH 7.6), after blocking for 2 h at 25 °C. .. After blocking, the membranes were incubated with the primary antibody at 4 °C overnight, followed by incubation with a horseradish peroxidase-conjugated secondary antibody at 25 °C for 3 h. The primary antibodies used were as follows: Occludin (Proteintech, 27260-1-AP, Wuhan, China), Claudin-1 (Santacruz, 3523011327, CA, USA), ZO-1 (Proteintech, 21773-1-AP, Wuhan, China), TLR-4 ( Proteintech, 66350-1-IG, Wuhan, China), Myd88 ( Proteintech, 23230-1-AP, Wuhan, China), NF-κB p65 (Proteintech, 10745-1-AP, Wuhan, China), Phospho-NF-κB p65 (Proteintech, 82335-1-RR, Wuhan, China), IkB (Proteintech, 10268-1-AP, Wuhan, China), Phospho-IkB (Proteintech, 82349-1-RR, Wuhan, China), Caspase8 (Santacruz,,#5800803, CA, USA), Caspase3 (Affinity, 00125840, Jiangsu, China), FADD (Affinity, #237743, Jiangsu, China), Cyt-c(Affinity, #73C2522, Jiangsu, China), BAX (Proteintech, 50599-2-Ig, Wuhan, China), Bcl-2 (Proteintech, 82469-6-RR, Wuhan, China), β-actin (Santacruz, #E2422, CA, USA). .. The secondary antibodies used were as follows: HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (Proteintech, SA00013-1, Wuhan, China)、 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (Proteintech, SA00013-2, Wuhan, China).

    Article Title: Theaflavin inhibits the malignant phenotype of human anaplastic thyroid cancer 8305C cells by regulating lipid metabolism via PI3K/AKT signaling
    Article Snippet: .. The PVDF membranes were blocked with 5% bovine serum albumin (BSA) for 1 h at room temperature and incubated with the following primary antibodies overnight at 4 °C: caspase3 (Proteintech, Wuhan, China, 19677-1-AP) (1:1,000), caspase8 (Proteintech, 13423-1-AP) (1:1,000), caspase9 (Proteintech, 10380-1-AP) (1:1,000), survivin (Proteintech, 10508-1-AP) (1:1,000), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, a control and the type of chemiluminescence detection reagent used) (Proteintech, 10494-1-AP) (1:1,000). ..

    other:

    Article Title: Targeting tumor metabolism: The dual attack of emodin on glycolysis and oxidative phosphorylation in esophageal squamous cell carcinoma.
    Article Snippet: Background: Esophageal squamous cell carcinoma (ESCC) exhibits elevated death rate and varying resistance to multiple chemotherapeutic agents.. Emodin, a key constituent of rhubarb, has demonstrated anticancer activities in colorectal and breast cancers.. However, its application and mechanism in ESCC await full clarification.

    Control:

    Article Title: Theaflavin inhibits the malignant phenotype of human anaplastic thyroid cancer 8305C cells by regulating lipid metabolism via PI3K/AKT signaling
    Article Snippet: .. The PVDF membranes were blocked with 5% bovine serum albumin (BSA) for 1 h at room temperature and incubated with the following primary antibodies overnight at 4 °C: caspase3 (Proteintech, Wuhan, China, 19677-1-AP) (1:1,000), caspase8 (Proteintech, 13423-1-AP) (1:1,000), caspase9 (Proteintech, 10380-1-AP) (1:1,000), survivin (Proteintech, 10508-1-AP) (1:1,000), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, a control and the type of chemiluminescence detection reagent used) (Proteintech, 10494-1-AP) (1:1,000). ..



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