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anti calr antibody  (Proteintech)


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    Structured Review

    Proteintech anti calr antibody
    <t>CALR</t> is overexpressed in osteosarcoma and modulates cellular phenotypes. (A) qRT-PCR analysis of CALR mRNA levels in osteosarcoma cell lines (MG-63, 143B, HOS) compared to human normal osteoblasts (hFOB1.19). (B) Western blot showing endogenous CALR protein expression in untreated osteosarcoma cells (Saos-2, MG-63, 143B, U2OS) and CALR-overexpressing 143B cells (OE-CALR). (C) Immunofluorescence staining of <t>CALR</t> <t>(TRITC,</t> red) and nuclei (DAPI, blue) in 143B cells under overexpression (OE-CALR), knockdown (sh-CALR), and negative control (NC) conditions. Fluorescence intensity quantification is shown below. *P < 0.05, **P < 0.01, ***P < 0.001.
    Anti Calr Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti calr antibody/product/Proteintech
    Average 93 stars, based on 26 article reviews
    anti calr antibody - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Identification and comprehensive analysis of an immune-related gene prognostic model for indicating tumor immune microenvironment features in soft tissue sarcoma"

    Article Title: Identification and comprehensive analysis of an immune-related gene prognostic model for indicating tumor immune microenvironment features in soft tissue sarcoma

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2025.1609501

    CALR is overexpressed in osteosarcoma and modulates cellular phenotypes. (A) qRT-PCR analysis of CALR mRNA levels in osteosarcoma cell lines (MG-63, 143B, HOS) compared to human normal osteoblasts (hFOB1.19). (B) Western blot showing endogenous CALR protein expression in untreated osteosarcoma cells (Saos-2, MG-63, 143B, U2OS) and CALR-overexpressing 143B cells (OE-CALR). (C) Immunofluorescence staining of CALR (TRITC, red) and nuclei (DAPI, blue) in 143B cells under overexpression (OE-CALR), knockdown (sh-CALR), and negative control (NC) conditions. Fluorescence intensity quantification is shown below. *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: CALR is overexpressed in osteosarcoma and modulates cellular phenotypes. (A) qRT-PCR analysis of CALR mRNA levels in osteosarcoma cell lines (MG-63, 143B, HOS) compared to human normal osteoblasts (hFOB1.19). (B) Western blot showing endogenous CALR protein expression in untreated osteosarcoma cells (Saos-2, MG-63, 143B, U2OS) and CALR-overexpressing 143B cells (OE-CALR). (C) Immunofluorescence staining of CALR (TRITC, red) and nuclei (DAPI, blue) in 143B cells under overexpression (OE-CALR), knockdown (sh-CALR), and negative control (NC) conditions. Fluorescence intensity quantification is shown below. *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence, Staining, Over Expression, Knockdown, Negative Control, Fluorescence



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    <t>CALR</t> is overexpressed in osteosarcoma and modulates cellular phenotypes. (A) qRT-PCR analysis of CALR mRNA levels in osteosarcoma cell lines (MG-63, 143B, HOS) compared to human normal osteoblasts (hFOB1.19). (B) Western blot showing endogenous CALR protein expression in untreated osteosarcoma cells (Saos-2, MG-63, 143B, U2OS) and CALR-overexpressing 143B cells (OE-CALR). (C) Immunofluorescence staining of <t>CALR</t> <t>(TRITC,</t> red) and nuclei (DAPI, blue) in 143B cells under overexpression (OE-CALR), knockdown (sh-CALR), and negative control (NC) conditions. Fluorescence intensity quantification is shown below. *P < 0.05, **P < 0.01, ***P < 0.001.
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    Image Search Results


    CALR is overexpressed in osteosarcoma and modulates cellular phenotypes. (A) qRT-PCR analysis of CALR mRNA levels in osteosarcoma cell lines (MG-63, 143B, HOS) compared to human normal osteoblasts (hFOB1.19). (B) Western blot showing endogenous CALR protein expression in untreated osteosarcoma cells (Saos-2, MG-63, 143B, U2OS) and CALR-overexpressing 143B cells (OE-CALR). (C) Immunofluorescence staining of CALR (TRITC, red) and nuclei (DAPI, blue) in 143B cells under overexpression (OE-CALR), knockdown (sh-CALR), and negative control (NC) conditions. Fluorescence intensity quantification is shown below. *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Frontiers in Oncology

    Article Title: Identification and comprehensive analysis of an immune-related gene prognostic model for indicating tumor immune microenvironment features in soft tissue sarcoma

    doi: 10.3389/fonc.2025.1609501

    Figure Lengend Snippet: CALR is overexpressed in osteosarcoma and modulates cellular phenotypes. (A) qRT-PCR analysis of CALR mRNA levels in osteosarcoma cell lines (MG-63, 143B, HOS) compared to human normal osteoblasts (hFOB1.19). (B) Western blot showing endogenous CALR protein expression in untreated osteosarcoma cells (Saos-2, MG-63, 143B, U2OS) and CALR-overexpressing 143B cells (OE-CALR). (C) Immunofluorescence staining of CALR (TRITC, red) and nuclei (DAPI, blue) in 143B cells under overexpression (OE-CALR), knockdown (sh-CALR), and negative control (NC) conditions. Fluorescence intensity quantification is shown below. *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Cells on coverslips were fixed with 4% PFA, permeabilized with 0.2% Triton X-100, blocked with 3% BSA, and incubated with anti-CALR antibody (#10208-1-AP, 1:200, Proteintech) overnight at 4°C, followed by TRITC-conjugated secondary antibody (1:500) and DAPI (1 μg/mL) at RT.

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence, Staining, Over Expression, Knockdown, Negative Control, Fluorescence