rabbit anti calpastatin (Proteintech)
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Proteintech
rabbit anti calpastatin
Rabbit Anti Calpastatin, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/calpastatin/Calpastatin+Antibody/pm41894401-103-1-4
Average 92 stars, based on 9 article reviews
Rabbit Anti Calpastatin, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/calpastatin/Calpastatin+Antibody/pm41894401-103-1-4
Average 92 stars, based on 9 article reviews
rabbit anti calpastatin - by Bioz Stars,
2026-10
92/100 stars
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Saline:Article Title: Quantitative proteomic analysis reveals that transmissible gastroenteritis virus activates the JAK-STAT1 signaling pathway. Article Snippet: Transmissible gastroenteritis virus (TGEV), a porcine enteropathogenic coronavirus, causes lethal watery diarrhea and severe dehydration in piglets.. In this study, liquid chromatography−tandem mass spectrometry coupled to isobaric tags for relative and absolute quantification labeling was used to quantitatively identify differentially expressed cellular proteins after TGEV infection in PK-15 cells.. In total, 162 differentially expressed cellular proteins were identified, including 60 upregulated proteins and 102 downregulated proteins. Article Title: Differential protein metabolism and regeneration in hypertrophic diaphragm and atrophic gastrocnemius muscles in hibernating Daurian ground squirrels. Article Snippet: Then the protein gel was transferred electrically to polyvinylidene difluoride (PVDF) membranes (0.45 μm) using a Bio-Rad semi-dry transfer apparatus. .. The blotted PVDF membranes were incubated with 1% bovine serum albumin in Tris-buffered saline (TBS; 50 mM Tris–HCl and 150 mM NaCl, pH 7.5) and P-Akt (Ser473) (1:1000; abcam; 81283), P-mTORC1 (Ser2448) (1:1000; Sigma; 4504476), P-S6K1 (Thr389) [1:1000; Cell Signaling Technology (CST); 9205S], P-4E-BP1 (Thr37/46) (1:1000; CST; 2855S), calpain-1 (1:1000; CST; 2556S), calpain-2 (1:1000; CST; 2539S), Incubation:Article Title: Quantitative proteomic analysis reveals that transmissible gastroenteritis virus activates the JAK-STAT1 signaling pathway. Article Snippet: Transmissible gastroenteritis virus (TGEV), a porcine enteropathogenic coronavirus, causes lethal watery diarrhea and severe dehydration in piglets.. In this study, liquid chromatography−tandem mass spectrometry coupled to isobaric tags for relative and absolute quantification labeling was used to quantitatively identify differentially expressed cellular proteins after TGEV infection in PK-15 cells.. In total, 162 differentially expressed cellular proteins were identified, including 60 upregulated proteins and 102 downregulated proteins. Article Title: Differential protein metabolism and regeneration in hypertrophic diaphragm and atrophic gastrocnemius muscles in hibernating Daurian ground squirrels. Article Snippet: Then the protein gel was transferred electrically to polyvinylidene difluoride (PVDF) membranes (0.45 μm) using a Bio-Rad semi-dry transfer apparatus. .. The blotted PVDF membranes were incubated with 1% bovine serum albumin in Tris-buffered saline (TBS; 50 mM Tris–HCl and 150 mM NaCl, pH 7.5) and P-Akt (Ser473) (1:1000; abcam; 81283), P-mTORC1 (Ser2448) (1:1000; Sigma; 4504476), P-S6K1 (Thr389) [1:1000; Cell Signaling Technology (CST); 9205S], P-4E-BP1 (Thr37/46) (1:1000; CST; 2855S), calpain-1 (1:1000; CST; 2556S), calpain-2 (1:1000; CST; 2539S), Article Title: Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis. Article Snippet: For western blot assay, 40 μg of protein lysates were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) (Beyotime, P0012AC) and transferred to polyvinylidene difluoride (PVDF) membranes (Biosharp, BS-PVDF-45/BS-PVDF-22). .. After blocking in 5% non-fat milk (Biosharp, BS102), PVDF membranes were then incubated overnight at 4 °C with primary antibodies and probed for Article Title: Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis Article Snippet: .. The membranes were blocked with 5% fat-free milk for 2 h at room temperature, then were incubated with primary antibodies against calpain-1 (1 : 1,000, ProteinTech), calpain-2 (1 : 1,000, ProteinTech), Article Title: Calpain-activated mTORC2/Akt pathway mediates airway smooth muscle remodelling in asthma. Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/cea.12805 This article is protected by copyright.. All rights reserved. Article Title: Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD. Article Snippet: Astrocytic functions and brain-derived neurotrophic factor (BDNF)-tyrosine kinase receptor B (TrkB) signaling pathways are impaired in stress-related neuropsychiatric diseases.. Previous studies have reported neuroprotective effects of 7,8-dihydroxyflavone (7,8-DHF), a TrkB activator.. Here, we investigated the molecular mechanisms underlying pathogenesis of post-traumatic stress disorder (PTSD) using a modified single-prolonged stress (SPS&S) model and the potential beneficial effects of 7,8-DHF. Article Title: Caspase-3 interactions with calpain and cathepsin L: Implications for protein stability and quality in fish fillets during postmortem storage Article Snippet: The degradation of structural proteins during fish postmortem storage is a critical factor affecting meat quality, leading to economic losses in the seafood industry.. The complex interplay between caspase-3 and structural protein stability during fish postmortem storage remains largely unexplored.. By treating grass carp fillets with a caspase-3 inhibitor, we establish a model for inhibiting apoptosis, delineating the role of caspase-3 in protein degradation. Blocking Assay:Article Title: Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis. Article Snippet: For western blot assay, 40 μg of protein lysates were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) (Beyotime, P0012AC) and transferred to polyvinylidene difluoride (PVDF) membranes (Biosharp, BS-PVDF-45/BS-PVDF-22). .. After blocking in 5% non-fat milk (Biosharp, BS102), PVDF membranes were then incubated overnight at 4 °C with primary antibodies and probed for Bioprocessing:Article Title: Calpain-activated mTORC2/Akt pathway mediates airway smooth muscle remodelling in asthma. Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/cea.12805 This article is protected by copyright.. All rights reserved. Article Title: Caspase-3 interactions with calpain and cathepsin L: Implications for protein stability and quality in fish fillets during postmortem storage Article Snippet: The degradation of structural proteins during fish postmortem storage is a critical factor affecting meat quality, leading to economic losses in the seafood industry.. The complex interplay between caspase-3 and structural protein stability during fish postmortem storage remains largely unexplored.. By treating grass carp fillets with a caspase-3 inhibitor, we establish a model for inhibiting apoptosis, delineating the role of caspase-3 in protein degradation. Software:Article Title: Calpain-activated mTORC2/Akt pathway mediates airway smooth muscle remodelling in asthma. Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/cea.12805 This article is protected by copyright.. All rights reserved. Polyacrylamide Gel Electrophoresis:Article Title: Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD. Article Snippet: Astrocytic functions and brain-derived neurotrophic factor (BDNF)-tyrosine kinase receptor B (TrkB) signaling pathways are impaired in stress-related neuropsychiatric diseases.. Previous studies have reported neuroprotective effects of 7,8-dihydroxyflavone (7,8-DHF), a TrkB activator.. Here, we investigated the molecular mechanisms underlying pathogenesis of post-traumatic stress disorder (PTSD) using a modified single-prolonged stress (SPS&S) model and the potential beneficial effects of 7,8-DHF. Membrane:Article Title: Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD. Article Snippet: Astrocytic functions and brain-derived neurotrophic factor (BDNF)-tyrosine kinase receptor B (TrkB) signaling pathways are impaired in stress-related neuropsychiatric diseases.. Previous studies have reported neuroprotective effects of 7,8-dihydroxyflavone (7,8-DHF), a TrkB activator.. Here, we investigated the molecular mechanisms underlying pathogenesis of post-traumatic stress disorder (PTSD) using a modified single-prolonged stress (SPS&S) model and the potential beneficial effects of 7,8-DHF. |