calpain2 (Proteintech)
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Proteintech
calpain2
Calpain2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/calpain2/Piezo1+(extracellular+domain)+Antibody/pmc13004862-362-52-71
Average 96 stars, based on 379 article reviews
Calpain2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/calpain2/Piezo1+(extracellular+domain)+Antibody/pmc13004862-362-52-71
Average 96 stars, based on 379 article reviews
calpain2 - by Bioz Stars,
2026-10
96/100 stars
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SDS Page:Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis Article Snippet: Protein concentration was determined with the BCA assay (beyotime, # P0010). .. 30–60 μg of protein was loaded per well on 4–20% SDS-PAGE gels (ACE, #ET15420LGel), transferred to ethanol-activated PVDF membranes (Thermo Fisher Scientific, #88518), blocked with 5% skim milk in TBST, and incubated overnight at 4 °C with primary antibodies diluted in TBST: IL-33 (R&D Systems, #AF3626, 1:1000), PIEZO1 (Proteintech, #15939-1-AP, 1:1 000), Incubation:Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis Article Snippet: Protein concentration was determined with the BCA assay (beyotime, # P0010). .. 30–60 μg of protein was loaded per well on 4–20% SDS-PAGE gels (ACE, #ET15420LGel), transferred to ethanol-activated PVDF membranes (Thermo Fisher Scientific, #88518), blocked with 5% skim milk in TBST, and incubated overnight at 4 °C with primary antibodies diluted in TBST: IL-33 (R&D Systems, #AF3626, 1:1000), PIEZO1 (Proteintech, #15939-1-AP, 1:1 000), Article Title: Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons. Article Snippet: The purpose of this study is to investigate whether calpastatin (CAST) plays an important role in the regulated necrosis (RN) in rat retinal neurons under an excessive glutamate condition and furthermore to investigate whether this process is regulated by calapin1 and calpain2.. In the present study, glutamate triggered CAST inhibition, calpain2 activation and retinal neuronal RN after injury.. The application of CAST active peptide could provide protective effects against activated calpain2 mediated RN. Article Title: CAPNS1/Dicer feedback loop facilitates glycolysis, proliferation, and metastasis in hepatocellular carcinoma cells by inhibiting the maturation of MicroRNA pool. Article Snippet: .. Following adhesion, the cells were treated with 4% paraformaldehyde for 30 minutes to fix them, followed by a 15- minute blocking step with 5% BSA, and then incubated with primary antibodies, including Article Title: Mechanosensitive Piezo1 channels mediate renal fibrosis Article Snippet: .. Western blotting was performed by electrophoresis and incubation with primary antibodies against fibronectin, α-SMA (ab5694, Abcam; BM0002, Boster; 14395-1-AP, Proteintech), collagen I (BA0325, Boster), Article Title: CAPNS1/Dicer feedback loop facilitates glycolysis, proliferation, and metastasis in hepatocellular carcinoma cells by inhibiting the maturation of MicroRNA pool Article Snippet: .. Following adhesion, the cells were treated with 4% paraformaldehyde for 30 min to fix them, followed by a 15-minute blocking step with 5% BSA, and then incubated with primary antibodies, including Blocking Assay:Article Title: CAPNS1/Dicer feedback loop facilitates glycolysis, proliferation, and metastasis in hepatocellular carcinoma cells by inhibiting the maturation of MicroRNA pool. Article Snippet: .. Following adhesion, the cells were treated with 4% paraformaldehyde for 30 minutes to fix them, followed by a 15- minute blocking step with 5% BSA, and then incubated with primary antibodies, including Article Title: CAPNS1/Dicer feedback loop facilitates glycolysis, proliferation, and metastasis in hepatocellular carcinoma cells by inhibiting the maturation of MicroRNA pool Article Snippet: .. Following adhesion, the cells were treated with 4% paraformaldehyde for 30 min to fix them, followed by a 15-minute blocking step with 5% BSA, and then incubated with primary antibodies, including Western Blot:Article Title: Mechanosensitive Piezo1 channels mediate renal fibrosis Article Snippet: .. Western blotting was performed by electrophoresis and incubation with primary antibodies against fibronectin, α-SMA (ab5694, Abcam; BM0002, Boster; 14395-1-AP, Proteintech), collagen I (BA0325, Boster), Electrophoresis:Article Title: Mechanosensitive Piezo1 channels mediate renal fibrosis Article Snippet: .. Western blotting was performed by electrophoresis and incubation with primary antibodies against fibronectin, α-SMA (ab5694, Abcam; BM0002, Boster; 14395-1-AP, Proteintech), collagen I (BA0325, Boster), |
