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fluorescence activated cell sorting facs assays calcein am  (MedChemExpress)


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    MedChemExpress fluorescence activated cell sorting facs assays calcein am
    Fluorescence Activated Cell Sorting Facs Assays Calcein Am, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein/Calcein-AM/pm42298670-157-0-8
    Average 96 stars, based on 105 article reviews
    fluorescence activated cell sorting facs assays calcein am - by Bioz Stars, 2026-09
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    Related Articles

    Mouse Assay:

    Article Title: Exposure to 6-PPD Quinone suppresses the proliferation and differentiation of osteoblasts by Nr4a1-p38 MAPK signal axis
    Article Snippet: 6-PPD quinone (6-PPDQ), a new environmental contaminant, has been frequently detected in different environmental matrices and widely identified as causing health hazard.. However, most early studies have primarily reported 6-PPDQ-induced toxic effects on visceral organs but do not consider its skeletal toxicity as well as the associated molecular mechanism after 6-PPDQ exposure, which remains largely unknown.. This study investigated the impacts of 6-PPDQ on skeleton in vitro and in vivo.

    Injection:

    Article Title: Exposure to 6-PPD Quinone suppresses the proliferation and differentiation of osteoblasts by Nr4a1-p38 MAPK signal axis
    Article Snippet: 6-PPD quinone (6-PPDQ), a new environmental contaminant, has been frequently detected in different environmental matrices and widely identified as causing health hazard.. However, most early studies have primarily reported 6-PPDQ-induced toxic effects on visceral organs but do not consider its skeletal toxicity as well as the associated molecular mechanism after 6-PPDQ exposure, which remains largely unknown.. This study investigated the impacts of 6-PPDQ on skeleton in vitro and in vivo.

    Article Title: Nrf2/Nlrp3 signaling in aging BMSCs: Traf6 intervention as a novel approach to osteoporosis treatment
    Article Snippet: After the transfection knockdown efficiency was determined to be satisfactory, mice in the D-Gal and LV-shtraf6 groups were injected subcutaneously with d -Galactose (HY–N0210, MCE, Shanghai, China) at 150 mg/kg/d [ ]. .. Five mice in each group were injected intraperitoneally with 10 mg/kg calcein (HY-D0040, MCE, Shanghai, China) 10 and 3 days before harvesting, and the undecalcified hard tissue sections were taken in time after taking the materials. ..

    Article Title: MMP-2 associated imbalance of VEGF/Endostatin is linked to suppression of the PI3K/AKT/HIF-1α pathway in steroid-induced osteonecrosis of femoral head
    Article Snippet: .. In the sixth week of modeling, mice received an intraperitoneal injection of Calcein (10 mg/kg, dissolved in 2% NaHCO3 solution; MedChemExpress, NJ, USA), followed by a second injection 6 days later. ..

    Article Title: BMP2@PDA-LDH Functionalized Coating on ZK60 Magnesium Alloy: Enhanced Corrosion Resistance and Promoted Bone Defect Regeneration
    Article Snippet: .. At 2 weeks post-surgery, rats were injected with calcein (10 mg/kg, MCE). .. At 8 weeks, specimens were fixed in 4% PFA, dehydrated, embedded in resin, sectioned (200 μm), ground (20–30 μm), and imaged via confocal laser scanning microscope (STELLARIS 5, Leica, Germany).

    Article Title: Sympathetic overactivity-induced type H endothelial cell senescence contributes to the impairment of vascularized osteogenesis by PKM2-mediated glycolysis in preclinical stages of Alzheimer's mice.
    Article Snippet: Alzheimer's disease (AD) is characterized by early-onset bone loss, yet the underlying mechanisms remain elusive.. Here, we demonstrated that sympathetic hyperactivity drives vascularized osteogenesis impairment in preclinical AD through a novel PKM2-glycolysis–mediated bone vascular endothelial cell (EC) senescence pathway.. Utilizing systematic transcriptome profiling complemented by in vitro functional assays and in vivo validation studies, we found that norepinephrine (NE)-induced PKM2 downregulation disrupts glycolytic flux, triggering mitochondrial dysfunction-induced senescence (MiDAS) in vascular ECs.

    other:

    Article Title: Fatty acid distribution in human milk triacylglycerols regulates lipid absorption through calcium-mediated mucus phase transition.
    Article Snippet: Calcium ions (Ca2+) and intestinal mucus act as dynamic barriers regulating lipid transport.. This study reveals that the sn-2 positional distribution of palmitic acid in triacylglycerols (TAGs) modulates mucin gelation and lipid diffusion via a calcium-dependent mechanism.. Under gastrointestinal pH, Ca2+ at 3–4 mM compacts mucin and restricts lipid diffusion, while >5 mM Ca2+ induces porous structures.



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    Image Search Results


    Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

    Journal: Bioactive Materials

    Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.048

    Figure Lengend Snippet: Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

    Article Snippet: A Calcein/PI Cell Viability/Cytotoxicity Assay Kit (Beyotime, China) was used to recognize the living and dead cells.

    Techniques: In Vitro, Staining, Migration, Negative Control, Cell Culture, Software, Control