Journal: iScience
Article Title: Otic organoids: A model to study spiral ganglion neuron characteristics in Tmprss3-deficiency
doi: 10.1016/j.isci.2025.114355
Figure Lengend Snippet: Characterization of SGN-like neurons and glial cells in day-70 otic organoids (A) Day-70 organoids stained for VGLUT1 (green), MAP2 (white), and TUBB3 (red), confirming glutamatergic identity of neurons with neuritic extensions. VGLUT1 shows punctate labeling along MAP2 + neurites, consistent with its synaptic localization. (B) Dissociated day-70 organoid showing a representative SGN-like cell with bipolar morphology. The soma gives rise to two opposing neurites, co-expressing MAP2 (white), TUBB3 (red), and VGLUT1 (green). (C) S100β + glial cells (red), marking supporting glial populations within the organoids. (D) GFAP + glial cells (red), confirming astrocytic-like glial populations in the organoids. (E–E′) PRH + (green), TH + (red), and MAP2 + (white) cells, consistent with type II SGNs. (F–F′) CALB2 + (green) and MAP2 + (white), characteristic of type Ia SGNs. (G–G′) PROX1 + neurons (red) with MAP2 + (white) morphology, consistent with type Ib SGNs. PROX1 labeling appears predominantly perinuclear with partial overlap to DAPI, consistent with its function as a transcription factor. (H–H′) POU4F1 + (green), NF200 + (red), and MAP2 + (white) cells, characteristic of type Ic SGNs. Representative images were obtained from n ≥ 3 organoids per time point from four independent differentiation experiments. Scale bars, 100 μm (C–H), 50 μm (A), 20 μm (B), 10 μm (E′–H′). Nuclei were counterstained with DAPI (blue). See also .
Article Snippet: CALB2 , Santa Cruz , Sc-365956.
Techniques: Staining, Labeling, Expressing
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