Journal: bioRxiv
Article Title: Single-cell atlas of pig-to-monkey kidney xenotransplantation reveals macrophage chimerism and an IFN-ε orchestrated graft protective immune niche
doi: 10.64898/2026.03.21.713416
Figure Lengend Snippet: (A) Haematoxylin and eosin-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of glomeruli (row 1), peritubular capillaries (row 2), and tubulointerstitial compartments (row 3) are shown. Black arrows show non-infiltrated peritubular capillaries (sections a2 and c2), and infiltrated peritubular capillaries (section b2 and d2). (B) Immunohistochemistry-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of IgM (row 1), IgG (row 2) and C4d (row 3) stainings are shown. (C) Immunofluorescence staining of infiltrated macrophages with signature proteins. Scale bars, 100 µm.
Article Snippet: The primary antibody panel including: anti-IgM (11016-1-AP, 1:100, Proteintech), anti-IgG (ab109489, 1:500, Abcam), C4d (22233-1-AP, 1:200, Proteintech), anti-CD31 (ab28364, 1:50, Abcam), anti-vWF (ab6994, 1:200, Abcam), anti-CD3 (GB12014, 1:1000, Servicebio) and anti-CD15 (YT0726, 1:200, Immunoway) for IHC; anti-CD68 (bs-0649R, 1:200, Bioss), anti-SPP1 (83341-1-RR, 1:200, Proteintech), anti-VEGFA (66828-1-Ig, 1:200, Proteintech), anti-PD-L1 (ab205921, 1:200, Abcam), anti-IDO1 (ab211017, 1:1000, Abcam), anti-ARG1 (66129-1-Ig, 1:200, Proteintech), anti-FCGR2B (NB100-65338, 1:100, Novus Biologicals), anti-CD8a (66868-1-Ig, 1:400, Proteintech), and anti-KLRD1 (84466-5-RR, 1:200, Proteintech) for multiplexed IF.
Techniques: Control, Immunohistochemistry, Immunofluorescence, Staining
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