c20a4 chondrocyte cell line  (Millipore)

Journal: Bioactive Materials

Article Title: In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones

doi: 10.1016/j.bioactmat.2025.04.013

Figure Lengend Snippet: The biocompatibility of the scaffolds. (A) Live/dead staining of chondrocytes seeded on different scaffolds at one, three, and five days (live cells: green, dead cells: red; Scale bar = 200 μm). (B) The relative fluorescence intensity of live (Calcein AM) and dead (PI) cells one day after cell seeding was analyzed by Image J. (n = 3) (C) The relative fluorescence intensity of live and dead cells five days after cell seeding was analyzed by Image J. (n = 3) (D) SEM of chondrocytes and scaffolds after three days of co-culture, scale bar = 100 μm. (E) Cell viability after co-culturing chondrocytes and scaffolds (n = 3). (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001).

Article Snippet: The six-well plate was rinsed with ultrapure water and allowed to dry, and then applied to C20A4 human chondrocytes (cell line, Merk) for days, followed by RNA extraction using Trizol.

Techniques: Staining, Fluorescence, Co-Culture Assay

Journal: Bioactive Materials

Article Title: In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones

doi: 10.1016/j.bioactmat.2025.04.013

Figure Lengend Snippet: Staining of the defect area at the early implantation stage. (A) Expression of CD86 (M1), CD206 (M2), and ALP in the bone layer 10 days after implantation, and (B) expression of CD86 (M1), CD206 (M2), and ALP in the bone layer 30 days after implantation. The H&E and Saf-O staining of Coll scaffold (C) and Dopa-Col II scaffold (D) 30 days after implantation. (E) The IHC staining of Col 10 of the Dopa-Col II scaffold 30 days after implantation. The large number of hypertrophic chondrocytes (blue box) and ossification in the center of hypertrophic chondrocytes (yellow box). (Scale bar = 350 μm).

Article Snippet: The six-well plate was rinsed with ultrapure water and allowed to dry, and then applied to C20A4 human chondrocytes (cell line, Merk) for days, followed by RNA extraction using Trizol.

Techniques: Staining, Expressing, Immunohistochemistry

Journal: Bioactive Materials

Article Title: In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones

doi: 10.1016/j.bioactmat.2025.04.013

Figure Lengend Snippet: In vivo and in vitro mechanism verification. (A) After days of culturing chondrocytes in the Blank and Coated six-well plates, the gene expression levels of cartilage-related markers COL2A1 and SOX9, hypertrophic cartilage markers RUNX2 and COL10, as well as cartilage endochondral ossification markers pathway IHH and PTH1R using qRT-PCR experiments (n = 3). IF staining of PTH1R(B), IHH(C), and IHC staining of Col 10 (D) after 5–15 days of chondrocyte culture. IF staining of VEGFR2(E) and IHC staining of CD31 (F) after 5–15 days of vascular endothelial cell culture. (G) Staining results of scaffolds after 15 days of chondrocyte culture, H&E, Saf-O, and IHC staining of Col2. Scale bar = 50μm. (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001).

Article Snippet: The six-well plate was rinsed with ultrapure water and allowed to dry, and then applied to C20A4 human chondrocytes (cell line, Merk) for days, followed by RNA extraction using Trizol.

Techniques: In Vivo, In Vitro, Gene Expression, Quantitative RT-PCR, Staining, Immunohistochemistry, Cell Culture

Journal: Pharmaceuticals

Article Title: Biological Activities of Deer Antler-Derived Peptides on Human Chondrocyte and Bone Metabolism

doi: 10.3390/ph17040434

Figure Lengend Snippet: Effects of different metal ions and dipeptide YR on osteoblast growth. Note: Osteoblast proliferation activity is represented as the cell number. Data are presented as means ± SD from three independent experiments. Significance was determined through a one-tailed test analysis, with * p < 0.05 and ** p < 0.005 indicating a significant difference between peptide 2 combined with different metal ions and the no-treatment condition.

Article Snippet: Human osteoblasts (hFOB1.19), sourced from the American Type Cell Collection (ATCC), and articular chondrocyte C20A4, obtained from MERCK (Darmstadt, Germany), were cultured as reported previously [ ].

Techniques: Activity Assay, One-tailed Test