c16 paf  (MedChemExpress)

Journal: iScience

Article Title: Detailed composition of wax esters in mouse sebum and the involvement of FAR2 and AWAT2

doi: 10.1016/j.isci.2026.114836

Figure Lengend Snippet: Impaired production of WEs containing very-long-chain FAls in Far2 KO mouse sebum (A) Conversion of non-hydroxy as well as α- or ω-OH acyl-CoAs to FAls or 1,α/1,ω-diols by FARs, and the metabolic pathways of these products. (B) Total RNAs were prepared from the skin of 8-week-old male C57BL/6 mice ( n = 3), and the expression levels of Far1 , Far2 , and the housekeeping gene Hprt1 were measured via quantitative real-time RT-PCR. Values presented are mean +SD of the mRNA levels relative to Hprt1 . (C and D) Photographs of a Far2 KO male mouse at 6 months of age showing partial hair loss (C) and at 9 months showing whitening hair (D), as well as WT male mice of the same age. (E) WT and Far2 KO male mice ( n = 3 each) were housed in the same cage for 2 weeks, after which mice of different genotypes were transferred to separate cages. Lipids were extracted from the hair of the mice 0 and 2 weeks (w) after they were separated, and the WmE with C18:1 FA/C26:0 FAl was quantified via LC-MS/MS. Values presented are mean quantities +SD (∗ p < 0.05; ∗∗ p < 0.01; Tukey’s test). (F–K) WT and Far2 KO male mice ( n = 3 each) at 5 weeks of age were transferred to separate cages and housed separately for 3 weeks. Lipids were extracted from the hair, and WmEs (F and G), 2α-WdiEs (H), 2ω-WdiEs (I), Chol-OAHFAs (J), and Chol-Es (K) were analyzed via LC-MS/MS. (F and G) Values are the quantities of WmEs containing C16:0 FA and the indicated FAl moieties (F) and those containing the indicated FA and long-chain (LC) or very-long-chain (VLC) FAl moieties (G). Values shown are means +SD (∗ p < 0.05; ∗∗ p < 0.01; Welch’s t test). The simplified structures of the WmEs with the analyzed moieties (FAl and/or FA) are shown. (H and I) Values are the quantities of 2α-WdiEs (H) and 2ω-WdiEs (I), both containing the indicated FA moieties. Values presented are mean quantities +SD (∗ p < 0.05; ∗∗ p < 0.01; Welch’s t test). The simplified structures of the 2α-WdiE (H) and 2ω-WdiE (I) with the analyzed FA moieties are shown. (J and K) Values are the means +SD of the total quantities of Chol-OAHFAs (J) and Chol-Es (K).

Article Snippet: C16:0 Cholesteryl- d 7 ester , Avanti Research , Cat#700149.

Techniques: Expressing, Quantitative RT-PCR, Liquid Chromatography with Mass Spectroscopy

Journal: iScience

Article Title: Detailed composition of wax esters in mouse sebum and the involvement of FAR2 and AWAT2

doi: 10.1016/j.isci.2026.114836

Figure Lengend Snippet: Impaired WmE and 2ω-WdiE production containing long-chain FAs in Awat2 KO mouse sebum (A) Pathways for the production of WmEs and 2ω-WdiEs by AWAT2. AWAT2 produces WmEs using an acyl-CoA and an FAl as substrates. 2ω-WdiEs are produced through an initial esterification reaction of an acyl-CoA with a 1,ω-diol to form diol-FAs, followed by a second esterification reaction of an acyl-CoA with a diol-FA. AWAT2 catalyzes either or both of these reactions. (B) Total RNAs were prepared from the skin of 8-week-old male C57BL/6 mice ( n = 3), and the expression levels of Awat1 , Awat2 , and the housekeeping gene Hprt1 were measured via quantitative real-time RT-PCR. Values presented are mean +SD of the mRNA levels relative to Hprt1 . (C) Photographs of a 9-month-old Awat2 KO male mouse showing partial hair loss (arrowheads) and a WT male mouse of the same age. (D–J) WT and Awat2 KO male mice ( n = 3 each) at 5 weeks of age were transferred to separate cages and housed separately for 3 weeks. Lipids were extracted from the hair, and WmEs (D–F), 2ω-WdiEs (G), 2α-WdiEs (H), Chol-OAHFAs (I), and Chol-Es (J) were analyzed via LC-MS/MS. (D–F) Values are the quantities of the WmEs containing the indicated FA moieties (D) and the WmEs containing C16:0 FA (E) or C16:1 FA (F) and the indicated FAl moieties (E and F). Values shown are means +SD (∗ p < 0.05; ∗∗ p < 0.01; Welch’s t test). The simplified structures of the WmEs with the analyzed moieties (FA or FAl) are shown. (G and H) Values are the quantities of the 2ω-WdiEs (G) and 2α-WdiEs (H) containing the indicated FA moieties. Values presented are mean quantities +SD (∗∗ p < 0.01; Welch’s t test). The simplified structures of the 2ω-WdiE (G) and 2α-WdiE (H) with the analyzed FA moieties are shown. (I and J) Values are the means +SD of the total quantities of Chol-OAHFAs (I) and Chol-Es (J).

Article Snippet: C16:0 Cholesteryl- d 7 ester , Avanti Research , Cat#700149.

Techniques: Produced, Expressing, Quantitative RT-PCR, Liquid Chromatography with Mass Spectroscopy

Journal: iScience

Article Title: Detailed composition of wax esters in mouse sebum and the involvement of FAR2 and AWAT2

doi: 10.1016/j.isci.2026.114836

Figure Lengend Snippet: Impaired WmE production in Awat2 KO mouse meibum (A–D) Lipids were extracted from the eyelids of WT and Awat2 male KO mice ( n = 4 each) at 6–10 weeks of age, and WmEs were analyzed via LC-MS/MS. Values are the quantities of the WmEs containing the indicated FA moieties (A), the sum of the quantities of WmEs containing a saturated (Sat) or mono-unsaturated (MonoU) FA or FAl moiety (B), the quantities of WmEs containing C16:0 FA (C) or C16:1 FA (D), and the indicated FAl moieties (C and D). Values shown are means +SD (∗ p < 0.05; ∗∗ p < 0.01; Welch’s t test). The simplified structures of the WmEs with the analyzed moieties (FA or FAl) are shown.

Article Snippet: C16:0 Cholesteryl- d 7 ester , Avanti Research , Cat#700149.

Techniques: Liquid Chromatography with Mass Spectroscopy