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c-7902  (Millipore)


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    Millipore c-7902
    C 7902, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c-7902/calcium+chloride/pm37626875-281-79-82
    Average 90 stars, based on 1 article reviews
    c-7902 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Reduction of Ca 2+ Entry by a Specific Block of KCa3.1 Channels Optimizes Cytotoxic Activity of NK Cells against T-ALL Jurkat Cells.
    Article Snippet: Product Supplier Catalog/ Specification Number Stock Concentration Solvent Anti-CD107a-FITC Biolegend 326810 NS NS Fluo-4 AM Invitrogen by Thermo Fisher Scientific F1221 2 mM DMSO Fura-2 AM Invitrogen by Thermo Fisher Scientific F14201 2 mM DMSO CellTracker Deep Red Invitrogen by Thermo Fisher Scientific C34565 2 mM DMSO Hoechst 33342 Thermo FisherScientific 62249 20 mM H2O Blockers, salts, buffers Monensin Sigma-Aldrich 22373-78-0 2 mM Methanol NS6180 Tocris 4864 10 mM DMSO MgTx Tocris 3563 10 mM H2O PBS Gibco 70013-032 10X H2O CaCl2 Sigma C-7902 1 M H2O MgCl2 Sigma M-2670-100G 1 M H2O KCl Sigma P-5405 NA H2O EGTA Sigma E-4378 100 mM H2O HEPES Sigma H-7523 NA H2O KOH Sigma P-5958 1 M H2O NaOH Sigma S-0899 1 M H2O

    Solvent:

    Article Title: Reduction of Ca 2+ Entry by a Specific Block of KCa3.1 Channels Optimizes Cytotoxic Activity of NK Cells against T-ALL Jurkat Cells.
    Article Snippet: Product Supplier Catalog/ Specification Number Stock Concentration Solvent Anti-CD107a-FITC Biolegend 326810 NS NS Fluo-4 AM Invitrogen by Thermo Fisher Scientific F1221 2 mM DMSO Fura-2 AM Invitrogen by Thermo Fisher Scientific F14201 2 mM DMSO CellTracker Deep Red Invitrogen by Thermo Fisher Scientific C34565 2 mM DMSO Hoechst 33342 Thermo FisherScientific 62249 20 mM H2O Blockers, salts, buffers Monensin Sigma-Aldrich 22373-78-0 2 mM Methanol NS6180 Tocris 4864 10 mM DMSO MgTx Tocris 3563 10 mM H2O PBS Gibco 70013-032 10X H2O CaCl2 Sigma C-7902 1 M H2O MgCl2 Sigma M-2670-100G 1 M H2O KCl Sigma P-5405 NA H2O EGTA Sigma E-4378 100 mM H2O HEPES Sigma H-7523 NA H2O KOH Sigma P-5958 1 M H2O NaOH Sigma S-0899 1 M H2O



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    5×10 5 HTB-10, HTB-11 and CHME-5 cells were subcultured in 25 cm 2 tissue culture flask 24 hrs before transduction, then removed the media, DPBS washed cells twice, added 0.5 mL vector suspension (1×10 7 IU/mL, MOI = 10, for HTB-11, MOI = 1) containing 8 µg/mL polybrene, and incubated at 37°C for 2 hours with gentle mixing every 15 minutes. Then aspirated vector suspension and added 4 mL of fresh growth medium and incubated at 37°C with 5% CO 2 . The medium was replaced 24 hours post-infection and observed cells on day 3 post-infection under fluorescence microscope (Nikon Eclipse TE2000-U). (A) Photomicrographs of LV-transduced neuronal cell lines showing GFP exression. NL: Normal Light. FL: Fluorescent Light. (B) Immunofluorescent staining of GFP in neuronal cell lines. NL: Normal Light. GFP: overlay of green fluorescence. TRITC: overlay of red fluorescence. (C) Moderate to high levels of secreted hBDNF in LV-transduced neuronal cells conditioned media quantified by ELISA. Capture and detection antibodies were rabbit anti-human BDNF, goat anti-human IgG-Biotin respectively. (D) Stable expression of hBDNF detected from transduced neuronal cells. ELISA was performed every 5 passages for 20 passages to assess long-term stable expression of hBDNF gene. (E) Accumulative expression of hBDNF detected in LV-transduced HTB-11 cells.

    Journal: PLoS ONE

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    doi: 10.1371/journal.pone.0082030

    Figure Lengend Snippet: 5×10 5 HTB-10, HTB-11 and CHME-5 cells were subcultured in 25 cm 2 tissue culture flask 24 hrs before transduction, then removed the media, DPBS washed cells twice, added 0.5 mL vector suspension (1×10 7 IU/mL, MOI = 10, for HTB-11, MOI = 1) containing 8 µg/mL polybrene, and incubated at 37°C for 2 hours with gentle mixing every 15 minutes. Then aspirated vector suspension and added 4 mL of fresh growth medium and incubated at 37°C with 5% CO 2 . The medium was replaced 24 hours post-infection and observed cells on day 3 post-infection under fluorescence microscope (Nikon Eclipse TE2000-U). (A) Photomicrographs of LV-transduced neuronal cell lines showing GFP exression. NL: Normal Light. FL: Fluorescent Light. (B) Immunofluorescent staining of GFP in neuronal cell lines. NL: Normal Light. GFP: overlay of green fluorescence. TRITC: overlay of red fluorescence. (C) Moderate to high levels of secreted hBDNF in LV-transduced neuronal cells conditioned media quantified by ELISA. Capture and detection antibodies were rabbit anti-human BDNF, goat anti-human IgG-Biotin respectively. (D) Stable expression of hBDNF detected from transduced neuronal cells. ELISA was performed every 5 passages for 20 passages to assess long-term stable expression of hBDNF gene. (E) Accumulative expression of hBDNF detected in LV-transduced HTB-11 cells.

    Article Snippet: The secondary antibody used was Donkey anti-Goat IgG with TRITC (Rockland, USA).Cells were visually examined and documented using an inverted fluorescence microscope (Nikon Eclipse TE2000-U) with a digital camera attachment.

    Techniques: Transduction, Plasmid Preparation, Incubation, Infection, Fluorescence, Microscopy, Staining, Enzyme-linked Immunosorbent Assay, Expressing