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Proteintech c parp
C Parp, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 821 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+parp/PARP1+Antibody/pm41874277-266-47-49
Average 96 stars, based on 821 article reviews
c parp - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Incubation:

Article Title: <p>MicroRNA-375 Targets ATG14 to Inhibit Autophagy and Sensitize Hepatocellular Carcinoma Cells to Sorafenib</p>
Article Snippet: Protein concentration was determined using a BCA Protein Assay kit (Generay, Shanghai, China), and proteins were separated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), then transferred onto PVDF membranes (Millipore). .. The membranes were incubated with primary antibodies against LC3, P62 (1:1000, Cell Signaling Technology, MA, USA), ATG14, Caspase-3, PARP, C-PARP, or β-actin (1:1000, Proteintech Group, Chicago, IL, USA), then blocked with 5% fat-free milk powder in TBST buffer at 4°C overnight. .. The membranes were incubated with secondary antibody (CWBIO; 1:5000 dilution) and bands were detected by enhanced chemiluminescence (Thermo Fisher Scientific) using an submit your manuscript | www.dovepress.com DovePress OncoTargets and Therapy 2020:133558 O nc oT ar ge ts a nd T he ra py d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 46 .1 61 .6 1.

Article Title: Coumarins from Jinhua Finger Citron: Separation by Liquid–Liquid Chromatography and Potential Antitumor Activity
Article Snippet: Subsequently, 20.0 g of protein was loaded into a 10% gel and transmembrane PVDF (Millipore, Burlington, MA, USA, IPVH00010). .. After sealing with 5% skim milk for 1 h at room temperature, membranes were incubated with primary antibodies against PARP (CST, Danvers, MA, USA, 9542S), c-PARP, and Bcl-2 (Proteintech, Rosemont, IL, USA, 12789-1-AP) overnight at 4 °C. ..

Article Title: Coumarins from Jinhua Finger Citron: Separation by Liquid-Liquid Chromatography and Potential Antitumor Activity.
Article Snippet: Subsequently, 20.0 g of protein was loaded into a 10% gel and transmembrane PVDF (Millipore, Burlington, MA, USA, IPVH00010). .. After sealing with 5% skim milk for 1 h at room temperature, membranes were incubated with primary antibodies against PARP (CST, Danvers, MA, USA, 9542S), c-PARP, and Bcl-2 (Proteintech, Rosemont, IL, USA, 12789-1-AP) overnight at 4 ◦C. ..

Control:

Article Title: Pan-Cancer Analysis of PGAM1 and Its Experimental Validation in Uveal Melanoma Progression
Article Snippet: .. Next, the membranes were probed separately overnight at 4 °C with primary antibodies against PGAM1 (Proteintech, 1:1000), c-PARP (Abways, 1:1000), E-cadherin (Proteintech, 1:1000), Bcl-2 (Proteintech, 1:1000), Snail (Proteintech, 1:1000), PD-L1 (Abcam, 1:1000), and GAPDH (Abways, 1:3000) as a loading control. ..

Polyacrylamide Gel Electrophoresis:

Article Title: Hsp70-Targeting Chimeras Enable Dual Proteasomal and Lysosomal Degradation of Intracellular and Extracellular Proteins.
Article Snippet: Developing targeted protein degradation (TPD) strategies with disease-specific mechanisms, modularity, and facile designability could ensure drug efficacy and selectivity.. Herein, a small-molecule, Hsp70-based targeted protein degradation platform, termed Hsp70TAC, is described that enables tumor-selective degradation of both intracellular and extracellular proteins through distinct cellular pathways.. By conjugating protein-of-interest (POI) ligands to Hsp70 inhibitors, Hsp70TACs exploits the chaperone functions of Hsp70 to enable protein degradation through both the ubiquitin-proteasome system and the endocytosislysosome pathway.



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Santa Cruz Biotechnology c parp
A heat maps showing the expression of RBPs with significant differences in GSE16089 (parental Saos2 cells vs. resistant Saos2 cells). B qPCR and WB assays were performed to detect the expression of ZFP36L1 and apoptosis marker c-caspase3 in MTX-resistant (143B-R) and parental (143B-P) 143B cells. C Representative IHC images of ZFP36L1, c-caspase3, and <t>c-PARP</t> in individual samples from among 37 OS specimens. Scale bar, 200 μm. D the percentage of samples with low or high ZFP36L1 expression compared to the expression of c-caspase3 and c-PARP. E Kaplan-Meier survival analysis was performed to investigate the association between ZFP36L1 expression levels and overall survival in OS patients. Photograph ( F ) of xenograft tumors from mice injected with 143B cells expressing different levels of ZFP36L1 after MTX treatment. Tumor weights ( G ) and volumes ( H ) were measured. I WB assays were performed to detect apoptosis-associated proteins in xenograft tumors grown in vivo. The data are shown as means ± SEMs; * p < 0.05, ** p < 0.01.
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Image Search Results


A heat maps showing the expression of RBPs with significant differences in GSE16089 (parental Saos2 cells vs. resistant Saos2 cells). B qPCR and WB assays were performed to detect the expression of ZFP36L1 and apoptosis marker c-caspase3 in MTX-resistant (143B-R) and parental (143B-P) 143B cells. C Representative IHC images of ZFP36L1, c-caspase3, and c-PARP in individual samples from among 37 OS specimens. Scale bar, 200 μm. D the percentage of samples with low or high ZFP36L1 expression compared to the expression of c-caspase3 and c-PARP. E Kaplan-Meier survival analysis was performed to investigate the association between ZFP36L1 expression levels and overall survival in OS patients. Photograph ( F ) of xenograft tumors from mice injected with 143B cells expressing different levels of ZFP36L1 after MTX treatment. Tumor weights ( G ) and volumes ( H ) were measured. I WB assays were performed to detect apoptosis-associated proteins in xenograft tumors grown in vivo. The data are shown as means ± SEMs; * p < 0.05, ** p < 0.01.

Journal: Cell Death & Disease

Article Title: Downregulation of ZFP36L1 contributes to methotrexate resistance in osteosarcoma through enhanced NHEJ DNA repair mechanisms

doi: 10.1038/s41419-025-08217-4

Figure Lengend Snippet: A heat maps showing the expression of RBPs with significant differences in GSE16089 (parental Saos2 cells vs. resistant Saos2 cells). B qPCR and WB assays were performed to detect the expression of ZFP36L1 and apoptosis marker c-caspase3 in MTX-resistant (143B-R) and parental (143B-P) 143B cells. C Representative IHC images of ZFP36L1, c-caspase3, and c-PARP in individual samples from among 37 OS specimens. Scale bar, 200 μm. D the percentage of samples with low or high ZFP36L1 expression compared to the expression of c-caspase3 and c-PARP. E Kaplan-Meier survival analysis was performed to investigate the association between ZFP36L1 expression levels and overall survival in OS patients. Photograph ( F ) of xenograft tumors from mice injected with 143B cells expressing different levels of ZFP36L1 after MTX treatment. Tumor weights ( G ) and volumes ( H ) were measured. I WB assays were performed to detect apoptosis-associated proteins in xenograft tumors grown in vivo. The data are shown as means ± SEMs; * p < 0.05, ** p < 0.01.

Article Snippet: Next, the sections were incubated with primary antibody against ZFP36L1 (Solarbio, #K110803P), c-PARP (Santa Cruz, #sc-56196), c-caspase3 (CST, #9661S), DCLRE1C (Santa Cruz, #sc-518193), and γ-H2AX (CST, #2577S) overnight at 4 °C.

Techniques: Expressing, Marker, Injection, In Vivo