Journal: Cell Death & Disease

Article Title: Downregulation of ZFP36L1 contributes to methotrexate resistance in osteosarcoma through enhanced NHEJ DNA repair mechanisms

doi: 10.1038/s41419-025-08217-4

Figure Lengend Snippet: A heat maps showing the expression of RBPs with significant differences in GSE16089 (parental Saos2 cells vs. resistant Saos2 cells). B qPCR and WB assays were performed to detect the expression of ZFP36L1 and apoptosis marker c-caspase3 in MTX-resistant (143B-R) and parental (143B-P) 143B cells. C Representative IHC images of ZFP36L1, c-caspase3, and c-PARP in individual samples from among 37 OS specimens. Scale bar, 200 μm. D the percentage of samples with low or high ZFP36L1 expression compared to the expression of c-caspase3 and c-PARP. E Kaplan-Meier survival analysis was performed to investigate the association between ZFP36L1 expression levels and overall survival in OS patients. Photograph ( F ) of xenograft tumors from mice injected with 143B cells expressing different levels of ZFP36L1 after MTX treatment. Tumor weights ( G ) and volumes ( H ) were measured. I WB assays were performed to detect apoptosis-associated proteins in xenograft tumors grown in vivo. The data are shown as means ± SEMs; * p < 0.05, ** p < 0.01.

Article Snippet: Next, the sections were incubated with primary antibody against ZFP36L1 (Solarbio, #K110803P), c-PARP (Santa Cruz, #sc-56196), c-caspase3 (CST, #9661S), DCLRE1C (Santa Cruz, #sc-518193), and γ-H2AX (CST, #2577S) overnight at 4 °C.

Techniques: Expressing, Marker, Injection, In Vivo