bt549  (ATCC)

Journal: Translational Oncology

Article Title: Piperlongumine suppresses YAP-ET-1-CXCL2 signaling to modulates aggressiveness of triple-negative breast cancer cells

doi: 10.1016/j.tranon.2026.102700

Figure Lengend Snippet: Piperlongumine (PL) exerts inhibitory effects on the growth of triple-negative breast cancer (TNBC) cells. (A) The chemical structure of PL. (B) MDA-MB-231 and BT549 TNBC cells were treated with various concentrations of PL (0, 1.25, 2.5, 5, 10, and 25 μ M) for 24, 48, and 72 h, and cell viability was measured by CCK8 assay. Data are presented as percentages relative to the untreated group, with values expressed as the mean ± standard deviation (SD) of three independent experiments. IC50 values were calculated. (C) Representative images of EdU-stained proliferative MDA-MB-231 and BT549 cells treated with PL for 24 h. Scale bar, 100 μ m. (D) Apoptosis of MDA-MB-231 and BT549 cells treated with PL for 24 h was detected by flow cytometry. (E) Western blot analysis of BAX and BCL2 expression (left) and cleaved-caspase 3 and cleaved-caspase 9 expression (right) in MDA-MB-231 and BT549 cells treated with PL for 24 h. Quantitative analysis was performed using ImageJ software. * p < 0.05, ** p < 0.01, and *** p < 0.001, as determined by unpaired t -tests.

Article Snippet: The human BT549 (RRID: CVCL_1092) and MDA-MB-231 (RRID: CVCL_0062) TNBC cell lines were purchased from American Type Culture Collection.

Techniques: CCK-8 Assay, Standard Deviation, Staining, Flow Cytometry, Western Blot, Expressing, Software

Journal: Translational Oncology

Article Title: Piperlongumine suppresses YAP-ET-1-CXCL2 signaling to modulates aggressiveness of triple-negative breast cancer cells

doi: 10.1016/j.tranon.2026.102700

Figure Lengend Snippet: Piperlongumine (PL) exerts inhibitory effects on the invasiveness of triple-negative breast cancer (TNBC) cells. (A) Transwell migration and invasion assays of BT549, MDA-MB-231, and 4T1 TNBC cells treated with PL (0, 1, and 5 μ M) for 24 h, with representative images (left) and quantitative analysis (right). Scale bar, 50 μ m. (B) Wound-healing assay of TNBC cells treated with PL for 24 h, with quantification of wound closure by ImageJ shown on the right. * p < 0.05, ** p < 0.01, *** p < 0.001 by unpaired t -test. Scale bar, 100 μ m. (C) Immunofluorescence staining of actin filaments in TNBC cells treated with PL (1 μ M) for 24 h using Alexa Fluor 488-conjugated phalloidin. Scale bar, 50 μ m. (D) Western blot analysis of epithelial–mesenchymal transition (EMT) marker expression in BT549 and MDA-MB-231 cells treated with PL for 24 h.

Article Snippet: The human BT549 (RRID: CVCL_1092) and MDA-MB-231 (RRID: CVCL_0062) TNBC cell lines were purchased from American Type Culture Collection.

Techniques: Migration, Wound Healing Assay, Immunofluorescence, Staining, Western Blot, Marker, Expressing

Journal: Translational Oncology

Article Title: Piperlongumine suppresses YAP-ET-1-CXCL2 signaling to modulates aggressiveness of triple-negative breast cancer cells

doi: 10.1016/j.tranon.2026.102700

Figure Lengend Snippet: Piperlongumine (PL) inhibits YAP signaling in triple-negative breast cancer (TNBC) cells. (A) Western blot analysis of YAP protein levels in MDA-MB-231 and BT549 cells treated with PL (0, 1, and 5 μ M) for 24 h. (B) Western blot analysis of YAP and phosphorylated YAP (p-YAP) expression treated with PL in different time points. (C) Western blot analysis of phosphorylated LATS (p-LATS) and phosphorylated AMPK (p-AMPK) at different time points after PL treatment. (D) Representative immunofluorescence images of YAP expression in BT549 and MDA-MB-231 cells treated with PL for 24 h at the indicated concentrations, with quantification of nuclear YAP-positive cells. Scale bar, 50 μ m. * p < 0.05 and ** p < 0.01, as determined by unpaired t -tests.

Article Snippet: The human BT549 (RRID: CVCL_1092) and MDA-MB-231 (RRID: CVCL_0062) TNBC cell lines were purchased from American Type Culture Collection.

Techniques: Western Blot, Expressing, Immunofluorescence

Journal: Translational Oncology

Article Title: Piperlongumine suppresses YAP-ET-1-CXCL2 signaling to modulates aggressiveness of triple-negative breast cancer cells

doi: 10.1016/j.tranon.2026.102700

Figure Lengend Snippet: ET-1 is a downstream of YAP and is suppressed by PL. (A) Real-time PCR analysis and Western blotting analysis of ET-1 and YAP expression levels in YAP-knockdown BT549 and MDA-MB-231 cells. (B) qPCR analysis of ET-1 mRNA levels in MDA-MB-231 and BT549 cells treated with PL (5 μ M) for 24 h. (C) Western blotting analysis of YAP, p-YAP (S61), and ET-1 protein levels in BT549 and MDA-MB-231 cells treated with increasing concentrations of PL for 24 h. (D) Representative immunofluorescence images of ET-1 expression in TNBC cells treated with PL (5 μ M) for 24 h. *** p < 0.001 as determined by unpaired t -tests. Scale bar, 50 μ m.

Article Snippet: The human BT549 (RRID: CVCL_1092) and MDA-MB-231 (RRID: CVCL_0062) TNBC cell lines were purchased from American Type Culture Collection.

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Expressing, Knockdown, Immunofluorescence