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bs‑0571r  (Bioss)


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    Bioss bs‑0571r
    Bs‑0571r, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bs%E2%80%910571r/CCNA2+Polyclonal+Antibody/pm34935054-65-155-157
    Average 92 stars, based on 3 article reviews
    bs‑0571r - by Bioz Stars, 2026-09
    92/100 stars

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    other:

    Article Title: Dichloromethane fractions of Calystegia soldanella induce S‑phase arrest and apoptosis in HT‑29 human colorectal cancer cells.
    Article Snippet: The antibodies used were as follows: anti‐Fas (cat. no. sc‐7886; rabbit; Santa cruz Biotechnology, inc.), anti‐caspase‐8 (cat. no. sc‐7890; rabbit; Santa cruz Biotechnology, inc.), anti‐Bcl‐2 (cat. no. sc‐7382; mouse; Santa cruz Biotechnology, inc.), anti‐Bcl‐extra‐large (xl; cat. no. sc‐7195; rabbit; Santa cruz Biotechnology, inc.), anti‐Bad (cat. no. sc‐8044; mouse; Santa cruz Biotechnology, inc.), anti‐Bax (cat. no. sc‐7480; mouse; Santa cruz Biotechnology, inc.), anti‐caspase‐9 (cat. no. sc‐7885; rabbit; Santa cruz Biotechnology, inc.), anti‐caspase‐7 (cat. no. sc‐6138; rabbit; Santa cruz Biotechnology, inc.), anti‐caspase‐3 (cat. no. sc‐7148; rabbit; Santa cruz Biotechnology, inc.), anti‐X‐linked inhibitor of apop‐ tosis protein (XiaP; cat. no. 2045; rabbit; cell Signaling Technology, inc.), anti‐cellular inhibitor of apoptosis protein (ciaP)‐1 (cat. no. 7065; rabbit; cell Signaling Technology, inc.), anti‐ciaP‐2 (cat. no. 3130; rabbit; cell Signaling Technology, inc.), anti‐cytochrome c (cat. no. 4272; rabbit; cell Signaling Technology, inc.), anti‐cytochrome c oxidase subunit iV (coX iV; cat. no. 4844; rabbit; cell Signaling Technology, inc.), anti‐cyclin a (cat. no. BS‐0571r; rabbit; BioSS), anti‐cdK2 (cat. no. sc‐163; rabbit; Santa cruz Biotechnology, inc.), anti‐cell division cycle 25 a (cdc25a; cat. no. sc‐7389; mouse; Santa cruz Biotechnology, inc.) and anti‐cyclin dependent kinase inhibitor 1 (p21; cat. no. sc‐271532; rabbit; Santa cruz Biotechnology, inc.). anti‐β‐actin (cat. no. sc‐47778; mouse; Santa cruz Biotechnology, inc.) antibody was used as the loading control.



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    DCM fraction treatment induces S-phase arrest in HT-29 cells. (A) HT-29 cells were incubated with various concentrations of the DCM fraction for 20 h. Flow cytometry was used to determine the percentages of cells in each phase of the cell cycle. The letters displayed above the concentrations represent significant differences (P<0.05) as determined by Bonferroni's multiple comparisons test; groups with different letters are significantly different to one another, whereas those with the same letter are not. (B) Protein expression levels of S-phase-related proteins cyclin A, CDK2, Cdc25A and p21 were measured using western blotting. All results were normalized to the untreated control (0 µg/ml). Data are presented as the mean ± SD of three independent experiments. *P<0.05 vs. 0 µg/ml DCM. DCM, dichloromethane; CDK2, cyclin-dependent kinase 2; Cdc25A, cell division cycle 25 homolog A; p21, cyclin dependent kinase inhibitor 1.
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    DCM fraction treatment induces S-phase arrest in HT-29 cells. (A) HT-29 cells were incubated with various concentrations of the DCM fraction for 20 h. Flow cytometry was used to determine the percentages of cells in each phase of the cell cycle. The letters displayed above the concentrations represent significant differences (P<0.05) as determined by Bonferroni's multiple comparisons test; groups with different letters are significantly different to one another, whereas those with the same letter are not. (B) Protein expression levels of S-phase-related proteins cyclin A, CDK2, Cdc25A and p21 were measured using western blotting. All results were normalized to the untreated control (0 µg/ml). Data are presented as the mean ± SD of three independent experiments. *P<0.05 vs. 0 µg/ml DCM. DCM, dichloromethane; CDK2, cyclin-dependent kinase 2; Cdc25A, cell division cycle 25 homolog A; p21, cyclin dependent kinase inhibitor 1.

    Journal: Molecular Medicine Reports

    Article Title: Dichloromethane fractions of Calystegia soldanella induce S-phase arrest and apoptosis in HT-29 human colorectal cancer cells

    doi: 10.3892/mmr.2021.12576

    Figure Lengend Snippet: DCM fraction treatment induces S-phase arrest in HT-29 cells. (A) HT-29 cells were incubated with various concentrations of the DCM fraction for 20 h. Flow cytometry was used to determine the percentages of cells in each phase of the cell cycle. The letters displayed above the concentrations represent significant differences (P<0.05) as determined by Bonferroni's multiple comparisons test; groups with different letters are significantly different to one another, whereas those with the same letter are not. (B) Protein expression levels of S-phase-related proteins cyclin A, CDK2, Cdc25A and p21 were measured using western blotting. All results were normalized to the untreated control (0 µg/ml). Data are presented as the mean ± SD of three independent experiments. *P<0.05 vs. 0 µg/ml DCM. DCM, dichloromethane; CDK2, cyclin-dependent kinase 2; Cdc25A, cell division cycle 25 homolog A; p21, cyclin dependent kinase inhibitor 1.

    Article Snippet: The antibodies used were as follows: Anti-Fas (cat. no. sc-7886; rabbit; Santa Cruz Biotechnology, Inc.), anti-caspase-8 (cat. no. sc-7890; rabbit; Santa Cruz Biotechnology, Inc.), anti-Bcl-2 (cat. no. sc-7382; mouse; Santa Cruz Biotechnology, Inc.), anti-Bcl-extra-large (xL; cat. no. sc-7195; rabbit; Santa Cruz Biotechnology, Inc.), anti-Bad (cat. no. sc-8044; mouse; Santa Cruz Biotechnology, Inc.), anti-Bax (cat. no. sc-7480; mouse; Santa Cruz Biotechnology, Inc.), anti-caspase-9 (cat. no. sc-7885; rabbit; Santa Cruz Biotechnology, Inc.), anti-caspase-7 (cat. no. sc-6138; rabbit; Santa Cruz Biotechnology, Inc.), anti-caspase-3 (cat. no. sc-7148; rabbit; Santa Cruz Biotechnology, Inc.), anti-X-linked inhibitor of apoptosis protein (XIAP; cat. no. 2045; rabbit; Cell Signaling Technology, Inc.), anti-cellular inhibitor of apoptosis protein (cIAP)-1 (cat. no. 7065; rabbit; Cell Signaling Technology, Inc.), anti-cIAP-2 (cat. no. 3130; rabbit; Cell Signaling Technology, Inc.), anti-cytochrome c (cat. no. 4272; rabbit; Cell Signaling Technology, Inc.), anti-cytochrome c oxidase subunit IV (COX IV; cat. no. 4844; rabbit; Cell Signaling Technology, Inc.), anti-cyclin A (cat. no. BS-0571R; rabbit; BIOSS), anti-CDK2 (cat. no. sc-163; rabbit; Santa Cruz Biotechnology, Inc.), anti-cell division cycle 25 A (Cdc25A; cat. no. sc-7389; mouse; Santa Cruz Biotechnology, Inc.) and anti-cyclin dependent kinase inhibitor 1 (p21; cat. no. sc-271532; rabbit; Santa Cruz Biotechnology, Inc.).

    Techniques: Incubation, Flow Cytometry, Expressing, Western Blot