cdk6i bsj  (MedChemExpress)

Journal: Journal of Advanced Research

Article Title: Histone deacetylases facilitate Th17-cell differentiation and pathogenicity in autoimmune uveitis via CDK6/ID2 axis

doi: 10.1016/j.jare.2024.07.029

Figure Lengend Snippet: CDK6 regulated a positive feedback loop with ID2 to promote Th17 pathogenicity and EAU development. A. Correlation heatmap showing the relative levels between downregulated rescue-TFs and CDK6. B. Venn diagram showing the intersection of downregulated rescue-TFs and downregulated rescue-DEGs. C. Venn diagram showing the intersection of ID2′s target genes and Top 10 Downregulated rescue-DEGs in PPI. D. The flow cytometry histograms (left) and column charts (right) showing the level of CDK6 in CD4 + IL-17A + Th17 cells among IRBP 1-20 , IRBP 1-20 + CDK6 siRNA, and IRBP 1-20 + CDK6i groups in vitro ( n = 5/group). E. The flow cytometry histograms (left) and column charts (right) showing the level of ID2 in CD4 + IL-17A + Th17 cells among IRBP 1-20 and IRBP 1-20 + ID2i groups in vitro ( n = 5/group). F. The flow cytometry histograms (left) and column charts (right) showing the percentage of the level of CDK6 in CD4 + IL-17A + Th17 cells among IRBP 1-20 , IRBP 1-20 + ID2i groups in vitro ( n = 5/group). G-H. The flow cytometry histograms (left) and column charts (right) showing the percentage of CD4 + IL-17A + Th17 cells ( G ), the level of PIM1 ( H ) in CD4 + IL-17A + Th17 cells among IRBP 1-20 , IRBP 1-20 + CDK6 siRNA, and IRBP 1-20 + CDK6i groups in vitro ( n = 5/group). I. The representative fundus examination images of EAU mice, CDK6i-treated EAU mice, and ID2i-treated EAU mice after immunization at day 14 (left). The white arrows indicate retinal inflammatory exudates. The column charts showing the clinical scores of the three groups ( n = 5/group, right). J. The representative H&E-stained images of EAU mice, CDK6i-treated EAU mice, and ID2i-treated EAU mice after immunization at day 14 (left). Scale bars: 50 μm. The black arrows indicate retinal folding. The column charts showing the pathology scores between the three groups ( n = 5/group, right). K-L. The flow cytometry histograms (left) and column charts (right) showing the percentage of retinal CD4 + cells ( K ) and CD4 + IL-17A + (Th17) ( L ) of EAU, CDK6i, and ID2i groups ( n = 5/group). Significance in D-E, G-L was calculated using one-way ANOVA test; significance in F was calculated using Mann-Whitney U test; *P<0.05, **P<0.01, ****P<0.0001.

Article Snippet: Isolated PBMCs were incubated with anti-human CD3/CD28 magnetic beads alone or with beads plus HDACi Belinostat (0.3 μM) with or without CDK6i BSJ-03–123 (3 μM, MedChem Express, cat. no. HY-111556) and ID2i helichrysetin (50 μM, TargetMol, cat. no. TN1727) , .

Techniques: Flow Cytometry, In Vitro, Staining, MANN-WHITNEY

Journal: Journal of Advanced Research

Article Title: Histone deacetylases facilitate Th17-cell differentiation and pathogenicity in autoimmune uveitis via CDK6/ID2 axis

doi: 10.1016/j.jare.2024.07.029

Figure Lengend Snippet: The HDACs/CDK6/ID2 axis is associated with AU progression and regulates the pathogenicity of human Th17 cells. A. The column charts showing the levels of HDAC1, pHDAC3, HDAC6, CDK6 and ID2 in human Th17 cells from HC, initial AU, relapsed AU, and recovery AU patients in flow cytometry analysis ( n = 6/group). B. The flow cytometry histograms (left) and column charts (right) showing the level of CDK6 in human CD3 + CD8 - T cells among Beads and Beads + CDK6i groups in vitro ( n = 5/group). C. The flow cytometry histograms (left) and column charts (right) showing the level of ID2 in human CD3 + CD8 - T cells among Beads and Beads + ID2i groups in vitro ( n = 5/group). D-G. The flow cytometry histograms (left) and column charts (right) showing the percentage of human Th17 cells ( D ), the level of GM-CSF ( E ), CDK6 ( F ), and ID2 ( G ) in human Th17 cells among Blank, Beads, Beads + HDACi, Beads + HDACi + CDK6i, and Beads + HDACi + ID2i groups in vitro ( n = 5/group). H-I. The flow cytometry histograms (left) and column charts (right) showing the level of Acetyl-H3 ( H ) and p53 ( I ) in human Th17 cells among Blank, Beads, Beads + HDACi groups in vitro ( n = 5/group). Significance in B-C was calculated using one-way ANOVA test; significance in D-I was calculated using Mann-Whitney U test; *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

Article Snippet: Isolated PBMCs were incubated with anti-human CD3/CD28 magnetic beads alone or with beads plus HDACi Belinostat (0.3 μM) with or without CDK6i BSJ-03–123 (3 μM, MedChem Express, cat. no. HY-111556) and ID2i helichrysetin (50 μM, TargetMol, cat. no. TN1727) , .

Techniques: Flow Cytometry, In Vitro, MANN-WHITNEY

Journal: iScience

Article Title: Cdk6’s functions are critically regulated by its unique C-terminus

doi: 10.1016/j.isci.2024.111697

Figure Lengend Snippet:

Article Snippet: BSJ-03-123 , MedChemExpress LLC , Cat# HY-111556.

Techniques: Recombinant, Luciferase, Magnetic Beads, Immunoprecipitation, Bicinchoninic Acid Protein Assay, Mass Spectrometry, Modification, SYBR Green Assay, Western Blot, Bradford Protein Assay, Software