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zeb2  (Bioss)


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    Bioss zeb2
    Figure 1. <t>ZEB2</t> promoted the activation of astrocyte after OGD/R in vitro. A: Western blot assay of ZEB2 expression in astrocyte. B: Quantification of Western blot band intensity. C: Immunofluorescence staining for GFAP (green fluorescence), ZEB2 (red fluores cence), and DAPI (blue) in astrocytes. D: Fluorescence intensity of ZEB2. E: Fluorescence intensity of GFAP. E: Fluorescence intensity of ZEB2. E: Fluorescence intensity of GFAP. (Error bars represent mean ± SD, Magnification:400×; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
    Zeb2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zeb2/product/Bioss
    Average 94 stars, based on 3 article reviews
    zeb2 - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Zinc finger E-Box binding homeobox 2 (ZEB2)-induced astrogliosis protected neuron from pyroptosis in cerebral ischemia and reperfusion injury."

    Article Title: Zinc finger E-Box binding homeobox 2 (ZEB2)-induced astrogliosis protected neuron from pyroptosis in cerebral ischemia and reperfusion injury.

    Journal: Bioengineered

    doi: 10.1080/21655979.2021.2012551

    Figure 1. ZEB2 promoted the activation of astrocyte after OGD/R in vitro. A: Western blot assay of ZEB2 expression in astrocyte. B: Quantification of Western blot band intensity. C: Immunofluorescence staining for GFAP (green fluorescence), ZEB2 (red fluores cence), and DAPI (blue) in astrocytes. D: Fluorescence intensity of ZEB2. E: Fluorescence intensity of GFAP. E: Fluorescence intensity of ZEB2. E: Fluorescence intensity of GFAP. (Error bars represent mean ± SD, Magnification:400×; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
    Figure Legend Snippet: Figure 1. ZEB2 promoted the activation of astrocyte after OGD/R in vitro. A: Western blot assay of ZEB2 expression in astrocyte. B: Quantification of Western blot band intensity. C: Immunofluorescence staining for GFAP (green fluorescence), ZEB2 (red fluores cence), and DAPI (blue) in astrocytes. D: Fluorescence intensity of ZEB2. E: Fluorescence intensity of GFAP. E: Fluorescence intensity of ZEB2. E: Fluorescence intensity of GFAP. (Error bars represent mean ± SD, Magnification:400×; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

    Techniques Used: Activation Assay, In Vitro, Western Blot, Expressing, Immunofluorescence, Staining, Fluorescence

    Figure 2. ZEB2 promoted astrogliosis after OGD/R in vitro. A: Western blot assay of ZEB2 and GFAP expression in astrocyte. B: Quantification of ZEB2 Western blot band intensity. C: Quantification of ZEB2 Western blot band intensity. (Error bars represent mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
    Figure Legend Snippet: Figure 2. ZEB2 promoted astrogliosis after OGD/R in vitro. A: Western blot assay of ZEB2 and GFAP expression in astrocyte. B: Quantification of ZEB2 Western blot band intensity. C: Quantification of ZEB2 Western blot band intensity. (Error bars represent mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

    Techniques Used: In Vitro, Western Blot, Expressing

    Figure 6. ZEB2 expression after AAV injection and MCAO/R model establishment. A. Western blot assay was used to evaluate the infection of AAV. B. Quantification of ZEB2 Western blot band intensity in each groups.
    Figure Legend Snippet: Figure 6. ZEB2 expression after AAV injection and MCAO/R model establishment. A. Western blot assay was used to evaluate the infection of AAV. B. Quantification of ZEB2 Western blot band intensity in each groups.

    Techniques Used: Expressing, Injection, Western Blot, Infection

    Figure 7. Overexpression of ZEB2 in brain tissues of MCAO/R rats promoted neuron regeneration by alleviating pyroptosis. A: Representative track images of each group mice. B: Mean escape latency time during the orientation navigation test on 1, 3,7,14,21,28 days after MCAO/R. C: Time of the rats stay in the target quadrant on 28 days after MCAO/R. D: mNSS score on 1, 3,7,14,21,28 days after MCAO/R. E: Representative images of brain slices stained by TTC in different groups at 28 days after MCAO/R. F: Staining by Nissl showed the Nissl bodies of hippocampus and cerebral cortex. G: Staining by Luxol fast blue (LFB) showed the myelin of hippocampus and cerebral cortex. H: Western blot analysis of the expression of pyroptosis protein i.e., caspase-1, IL-1β, NLRP3, gasdermin, cleaved N-terminal GSDMD in brain tissue. I: Relative protein expression of caspase-1. J. Relative protein expression of IL-1β. K: Relative protein expression of NLRP3. L: Relative protein expression of gasdermin, M: Relative protein expression of cleaved N-terminal GSDMD. N: ELISA analysis of the expression of IL-1β in brain tissue of rats on 28 days after MCAO/R. O: ELISA analysis of the expression of IL-18 in brain tissue of rats on 28 days after MCAO/R. (Error bars represent mean ± SD, Magnification:400×; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Figure Legend Snippet: Figure 7. Overexpression of ZEB2 in brain tissues of MCAO/R rats promoted neuron regeneration by alleviating pyroptosis. A: Representative track images of each group mice. B: Mean escape latency time during the orientation navigation test on 1, 3,7,14,21,28 days after MCAO/R. C: Time of the rats stay in the target quadrant on 28 days after MCAO/R. D: mNSS score on 1, 3,7,14,21,28 days after MCAO/R. E: Representative images of brain slices stained by TTC in different groups at 28 days after MCAO/R. F: Staining by Nissl showed the Nissl bodies of hippocampus and cerebral cortex. G: Staining by Luxol fast blue (LFB) showed the myelin of hippocampus and cerebral cortex. H: Western blot analysis of the expression of pyroptosis protein i.e., caspase-1, IL-1β, NLRP3, gasdermin, cleaved N-terminal GSDMD in brain tissue. I: Relative protein expression of caspase-1. J. Relative protein expression of IL-1β. K: Relative protein expression of NLRP3. L: Relative protein expression of gasdermin, M: Relative protein expression of cleaved N-terminal GSDMD. N: ELISA analysis of the expression of IL-1β in brain tissue of rats on 28 days after MCAO/R. O: ELISA analysis of the expression of IL-18 in brain tissue of rats on 28 days after MCAO/R. (Error bars represent mean ± SD, Magnification:400×; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Techniques Used: Over Expression, Staining, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay



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