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claudin 10  (Bioss)


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    Bioss claudin 10
    The expression of tight junction molecules in IL-22 −/− and WT mice. (A) IL-22 −/− , n = 6, and WT, n = 5, mouse uterine samples were harvested 48 h post intraperitoneal LPS injection. Total mRNA samples were extracted and processed by qRT-PCR to quantify the expression of tight junction molecules. The data are provided as mean ± SEM from three independent experiments. (B) IL-22 −/− , n = 6, and WT, n = 5, mouse uterine samples were harvested 48 h post intraperitoneal LPS injection, frozen fixed, and processed for IHC analysis. Original magnification, ×40; scale bar, 100 µm. H-score of tight junctions was calculated to measure whether the difference in the staining between the groups is statistically significant. The data from t-test are provided as mean ± SEM. (C–F) IF analysis of claudins’ expression in WT (C, E) and IL-22 −/− mice (D, F) . Claudin-2 staining is shown in panels (C , D) and <t>claudin-10</t> staining in panels E, F Small panels on the left demonstrate (from top to bottom) the following: nuclei staining in DAPI, blue; pan-cytokeratin staining in FITC, green; claudin-2 or claudin-10 in TRITC, red; merged image. Original magnification, ×10. Large panels demonstrate insets shown on the merged images; original magnification, ×20. Scale bar is 100 µm. WT, wild type; IL-22 −/− , IL-22 knockout; H-score, histological score; LPS, lipopolysaccharide; IHC, immunohistochemistry; IF, immunofluorescence; FITC, fluorescein isothiocyanate. * p < 0.05.
    Claudin 10, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/claudin 10/product/Bioss
    Average 92 stars, based on 2 article reviews
    claudin 10 - by Bioz Stars, 2026-02
    92/100 stars

    Images

    1) Product Images from "IL-22 regulates endometrial regeneration by enhancing tight junctions and orchestrating extracellular matrix"

    Article Title: IL-22 regulates endometrial regeneration by enhancing tight junctions and orchestrating extracellular matrix

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.955576

    The expression of tight junction molecules in IL-22 −/− and WT mice. (A) IL-22 −/− , n = 6, and WT, n = 5, mouse uterine samples were harvested 48 h post intraperitoneal LPS injection. Total mRNA samples were extracted and processed by qRT-PCR to quantify the expression of tight junction molecules. The data are provided as mean ± SEM from three independent experiments. (B) IL-22 −/− , n = 6, and WT, n = 5, mouse uterine samples were harvested 48 h post intraperitoneal LPS injection, frozen fixed, and processed for IHC analysis. Original magnification, ×40; scale bar, 100 µm. H-score of tight junctions was calculated to measure whether the difference in the staining between the groups is statistically significant. The data from t-test are provided as mean ± SEM. (C–F) IF analysis of claudins’ expression in WT (C, E) and IL-22 −/− mice (D, F) . Claudin-2 staining is shown in panels (C , D) and claudin-10 staining in panels E, F Small panels on the left demonstrate (from top to bottom) the following: nuclei staining in DAPI, blue; pan-cytokeratin staining in FITC, green; claudin-2 or claudin-10 in TRITC, red; merged image. Original magnification, ×10. Large panels demonstrate insets shown on the merged images; original magnification, ×20. Scale bar is 100 µm. WT, wild type; IL-22 −/− , IL-22 knockout; H-score, histological score; LPS, lipopolysaccharide; IHC, immunohistochemistry; IF, immunofluorescence; FITC, fluorescein isothiocyanate. * p < 0.05.
    Figure Legend Snippet: The expression of tight junction molecules in IL-22 −/− and WT mice. (A) IL-22 −/− , n = 6, and WT, n = 5, mouse uterine samples were harvested 48 h post intraperitoneal LPS injection. Total mRNA samples were extracted and processed by qRT-PCR to quantify the expression of tight junction molecules. The data are provided as mean ± SEM from three independent experiments. (B) IL-22 −/− , n = 6, and WT, n = 5, mouse uterine samples were harvested 48 h post intraperitoneal LPS injection, frozen fixed, and processed for IHC analysis. Original magnification, ×40; scale bar, 100 µm. H-score of tight junctions was calculated to measure whether the difference in the staining between the groups is statistically significant. The data from t-test are provided as mean ± SEM. (C–F) IF analysis of claudins’ expression in WT (C, E) and IL-22 −/− mice (D, F) . Claudin-2 staining is shown in panels (C , D) and claudin-10 staining in panels E, F Small panels on the left demonstrate (from top to bottom) the following: nuclei staining in DAPI, blue; pan-cytokeratin staining in FITC, green; claudin-2 or claudin-10 in TRITC, red; merged image. Original magnification, ×10. Large panels demonstrate insets shown on the merged images; original magnification, ×20. Scale bar is 100 µm. WT, wild type; IL-22 −/− , IL-22 knockout; H-score, histological score; LPS, lipopolysaccharide; IHC, immunohistochemistry; IF, immunofluorescence; FITC, fluorescein isothiocyanate. * p < 0.05.

    Techniques Used: Expressing, Injection, Quantitative RT-PCR, Staining, Knock-Out, Immunohistochemistry, Immunofluorescence

    The schematic presentation of the impact of IL-22 on endometrial regeneration. In comparison to WT animals, IL-22 −/− mice have lower expression of tight junctions and mucin-1. Decreased amount of tight junctions leads to permeability reduction. Cellular density of stromal compartment in IL-22 −/− mice is lower than in WT mice. This might be explained by disrupted permeability of tight junctions, which fail to adequately facilitate paracellular flux (shown in green dotted double-headed arrow in WT mice). This might lead to stromal edema that occurred in IL-22 −/− mice post endotoxin-induced abortion. Hence, IL-22 enhances paracellular flux performed by tight junctions (claudin-2 and claudin-10) and upregulates cell surface pathogen protectors (mucin-1) contributing to proper regeneration of endometrial layers after inflammation-triggered abortion. IL-22 also participates in the remodeling of the uterine tissue in inflammatory environment by regulating other cell–cell contacts called adherens junctions (E- and N-cadherin; not shown on the scheme). Therefore, IL-22 can be considered to perform a dual role: enhance uterine regeneration postpartum and barrier/protect against secondary infertility caused by inflammation. LPS, lipopolysaccharide; WT, wild type; IL-22 −/− , IL-22 knockout; Na, sodium; H 2 O, water.
    Figure Legend Snippet: The schematic presentation of the impact of IL-22 on endometrial regeneration. In comparison to WT animals, IL-22 −/− mice have lower expression of tight junctions and mucin-1. Decreased amount of tight junctions leads to permeability reduction. Cellular density of stromal compartment in IL-22 −/− mice is lower than in WT mice. This might be explained by disrupted permeability of tight junctions, which fail to adequately facilitate paracellular flux (shown in green dotted double-headed arrow in WT mice). This might lead to stromal edema that occurred in IL-22 −/− mice post endotoxin-induced abortion. Hence, IL-22 enhances paracellular flux performed by tight junctions (claudin-2 and claudin-10) and upregulates cell surface pathogen protectors (mucin-1) contributing to proper regeneration of endometrial layers after inflammation-triggered abortion. IL-22 also participates in the remodeling of the uterine tissue in inflammatory environment by regulating other cell–cell contacts called adherens junctions (E- and N-cadherin; not shown on the scheme). Therefore, IL-22 can be considered to perform a dual role: enhance uterine regeneration postpartum and barrier/protect against secondary infertility caused by inflammation. LPS, lipopolysaccharide; WT, wild type; IL-22 −/− , IL-22 knockout; Na, sodium; H 2 O, water.

    Techniques Used: Expressing, Permeability, Knock-Out



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