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brd3  (Bethyl)


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    Bethyl brd3
    Brd3, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brd3/product/Bethyl
    Average 95 stars, based on 106 article reviews
    brd3 - by Bioz Stars, 2026-02
    95/100 stars

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    The effect of MIA on the expression of BET proteins in the hippocampus. PIC (20 mg/kg b.w.) was injected intraperitoneally at gestation day 17 to female mice. Twelve-month-old offspring male mice were decapitated, and the brain tissue was collected. (A) The levels of mRNA for Brd2 , Brd3 , and Brd4 genes in the hippocampus were measured by using qPCR and calculated by the ΔΔCt method. An averaged Ct-value from three various reference genes ( Gusb , Hprt , Rn18s ) was used for calculation. (B) The levels of Brd2, Brd3, and Brd4 proteins in the hippocampus were determined by using ELISA assays and normalized to the total protein level. The presented data are means ± SEM. *** p < 0.001, compared with the control group. N = 7–8 (A) and 6 (B) . Each data point (○) represents an individual animal. Statistical analyses were conducted using two-way ANOVA followed by the Bonferroni post hoc test, selected based on data distribution.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Inhibition of BET proteins modulates amyloid-beta accumulation and cognitive performance in middle-aged mice prenatally exposed to maternal immune activation

    doi: 10.3389/fnmol.2025.1619583

    Figure Lengend Snippet: The effect of MIA on the expression of BET proteins in the hippocampus. PIC (20 mg/kg b.w.) was injected intraperitoneally at gestation day 17 to female mice. Twelve-month-old offspring male mice were decapitated, and the brain tissue was collected. (A) The levels of mRNA for Brd2 , Brd3 , and Brd4 genes in the hippocampus were measured by using qPCR and calculated by the ΔΔCt method. An averaged Ct-value from three various reference genes ( Gusb , Hprt , Rn18s ) was used for calculation. (B) The levels of Brd2, Brd3, and Brd4 proteins in the hippocampus were determined by using ELISA assays and normalized to the total protein level. The presented data are means ± SEM. *** p < 0.001, compared with the control group. N = 7–8 (A) and 6 (B) . Each data point (○) represents an individual animal. Statistical analyses were conducted using two-way ANOVA followed by the Bonferroni post hoc test, selected based on data distribution.

    Article Snippet: The level of mRNA for selected genes was analyzed using commercially available TaqMan Gene Expression Assays: Abca1 (Mm00442646_m1), Adam10 (Mm00545742_m1), Aph1b (Mm00781167_m1), App (Mm01344172_m1), Arg1 (Mm00475988_m1), Bace1 (Mm00478664_m1), Brd2 (Mm01271171_g1), Brd3 (Mm01326697_m1), Brd4 (Mm01350417_m1), Gusb (Mm01197698_m1), Hprt (Mm00446968_m1), Il1b (Mm00434228_m1), Il6 (Mm00446190_m1), Mme (Mm01285049_m1), Ncstn (Mm00452010_m1), Nos2 (Mm00440502_m1), Psen1 (Mm00501184_m1), Psen2 (Mm00448405_m1), Rn18s (Mm03928990_m1), Tnf (Mm00443258_m1).

    Techniques: Expressing, Injection, Enzyme-linked Immunosorbent Assay, Control