brcamp  (MedChemExpress)

Journal: Cell reports

Article Title: Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells

doi: 10.1016/j.celrep.2020.107711

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: To test endothelial cell function, human neonatal microvascular endothelial cells (Lonza, Walkersville, MD) or human iPSC-VECs (iCell Endothelial Cells) (Cellular Dynamics, Fujifilm, Madison, WI) in culture (2D) and assembled vasculature of liver scaffolds were incubated in EBM PLUS Basal Medium supplemented with EGM-2 SingleQuots Kit (Lonza, Walkersville, MD) for human neonatal microvascular endothelial cells and VascuLife VEGF Medium (Cell Technologies, Fremont, CA) supplemented with iCell Endothelial Cells Medium Supplement (Cellular Dynamics, Fujifilm, Madison, WI) for human iPS-derived endothelial cells containing either 1pM of 1a,25-dihydroxyvitamin D3 (Sigma-Aldrich, Saint Louis, MO) or 1 μM retinoic acid (Sigma-Aldrich, Saint Louis, MO) or 1mM Sp-8-BrcAMPs (BIOLOG, Life Science Institute, Bremen, Germany), 1 μM or 100nM Phorbol myristate acetate (PMA) (Sigma-Aldrich, Saint Louis, MO) and a combination of 100nM PMA/10 mM Forskolin (Sigma-Aldrich, Saint Louis, MO) for 24 h. Human tissue plasminogen activator was measured using an ELISA, according to the manufacturer’s instructions (Abcam, Cambridge, MA).

Techniques: Recombinant, Concentration Assay, Knock-Out, Membrane, SYBR Green Assay, Acid Assay, MTT Assay, DNA Purification, Enzyme-linked Immunosorbent Assay, Hydroxyproline Assay, Colorimetric Assay, Binding Assay, Software, Micro-CT, Transmission Assay, Microscopy, Micro-MRI, High Performance Liquid Chromatography