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bn82002 (MedChemExpress)

Name: bn82002
Brand: MedChemExpress
Rating: 90 (1 reviews)

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MedChemExpress bn82002
Bn82002, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bn82002/product/MedChemExpress
Average 90 stars, based on 1 article reviews

bn82002 - by Bioz Stars, 2026-03

90/100 stars

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( A ) BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were incubated for 30 min or 2 h in the presence or absence of GalXM-FLUOS (10 µg/ml). After incubation, cells were analyzed by FACScan flow cytometry. Fluorescence histograms of positive cells, from one representative experiment out of five with similar results, were reported. ( B ) In selected experiments, cells were incubated for 30 min as described above and subsequently examined under fluorescent light microscopy. Evans' Blue was used as a counterstain. Note the green fluorescence of GalXM-treated cells.

Journal: PLoS ONE

Article Title: Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage

doi: 10.1371/journal.pone.0012720

Figure Lengend Snippet: ( A ) BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were incubated for 30 min or 2 h in the presence or absence of GalXM-FLUOS (10 µg/ml). After incubation, cells were analyzed by FACScan flow cytometry. Fluorescence histograms of positive cells, from one representative experiment out of five with similar results, were reported. ( B ) In selected experiments, cells were incubated for 30 min as described above and subsequently examined under fluorescent light microscopy. Evans' Blue was used as a counterstain. Note the green fluorescence of GalXM-treated cells.

Article Snippet: CD45 tyrosine phosphatase inhibitor BN82002 hydrochloride salt (BN82002) was obtained from Sigma-Aldrich.

Techniques: Incubation, Flow Cytometry, Fluorescence, Light Microscopy

BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were incubated for 30 min, 2 h or 18 h in the presence or absence of GalXM (10 µg/ml). After incubation, cells were labelled with RPE mAb to CD45 and analyzed by FACScan flow cytometry. The mean of fluorescence intensity (MFI) of labelled cells is shown as a histogram ( A ) or is shown as FACScan histograms ( B ). Error bars denote s.e.m. *, p <0.05 (GalXM-treated vs untreated, n = 7). In selected experiments, cells (1×10 6 /ml) were incubated for 30 min in the presence or absence of GalXM-FLUOS (10 µg/ml). After incubation, cells were labelled with RPE mAb to CD45 and analyzed by FACScan flow cytometry. Dot plots of the percentage of double positive cells, from one representative experiment out of five with similar results, were reported ( C ). Cells (1×10 6 /ml) were incubated for 30 min with GalXM-FLUOS (10 µg/ml) (green), labelled with RPE mAb to CD45 (red), stained with DAPI (blue) and subsequently examined under fluorescent light microscopy (D).

Journal: PLoS ONE

Article Title: Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage

doi: 10.1371/journal.pone.0012720

Figure Lengend Snippet: BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were incubated for 30 min, 2 h or 18 h in the presence or absence of GalXM (10 µg/ml). After incubation, cells were labelled with RPE mAb to CD45 and analyzed by FACScan flow cytometry. The mean of fluorescence intensity (MFI) of labelled cells is shown as a histogram ( A ) or is shown as FACScan histograms ( B ). Error bars denote s.e.m. *, p <0.05 (GalXM-treated vs untreated, n = 7). In selected experiments, cells (1×10 6 /ml) were incubated for 30 min in the presence or absence of GalXM-FLUOS (10 µg/ml). After incubation, cells were labelled with RPE mAb to CD45 and analyzed by FACScan flow cytometry. Dot plots of the percentage of double positive cells, from one representative experiment out of five with similar results, were reported ( C ). Cells (1×10 6 /ml) were incubated for 30 min with GalXM-FLUOS (10 µg/ml) (green), labelled with RPE mAb to CD45 (red), stained with DAPI (blue) and subsequently examined under fluorescent light microscopy (D).

Article Snippet: CD45 tyrosine phosphatase inhibitor BN82002 hydrochloride salt (BN82002) was obtained from Sigma-Aldrich.

Techniques: Incubation, Flow Cytometry, Fluorescence, Staining, Light Microscopy

Fluorescence microscopy analysis of BW5147 and BW5147 (T200 − ) cells incubated for 2 h in the presence or absence (NS) of GalXM ( A ) or GalXM-FLUOS (green) ( B ) (both 10 µg/ml). After incubation, cells were labelled with RPE mAb to CD45 (red) and then examined under fluorescent light microscopy in the presence of DAPI (blue). Note the CD45 segregation in BW5147 cells treated with GalXM ( A ). The colocalization of GalXM with CD45 and the receptor segregation on BW5147 cells was demonstrated in the merged image of panel B ( B ). Original magnification 100x.

Journal: PLoS ONE

Article Title: Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage

doi: 10.1371/journal.pone.0012720

Figure Lengend Snippet: Fluorescence microscopy analysis of BW5147 and BW5147 (T200 − ) cells incubated for 2 h in the presence or absence (NS) of GalXM ( A ) or GalXM-FLUOS (green) ( B ) (both 10 µg/ml). After incubation, cells were labelled with RPE mAb to CD45 (red) and then examined under fluorescent light microscopy in the presence of DAPI (blue). Note the CD45 segregation in BW5147 cells treated with GalXM ( A ). The colocalization of GalXM with CD45 and the receptor segregation on BW5147 cells was demonstrated in the merged image of panel B ( B ). Original magnification 100x.

Article Snippet: CD45 tyrosine phosphatase inhibitor BN82002 hydrochloride salt (BN82002) was obtained from Sigma-Aldrich.

Techniques: Fluorescence, Microscopy, Incubation, Light Microscopy

( A ) BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were incubated for 18 h in the presence or absence (NS) of PHA (10 µg/ml) or GalXM (10 µg/ml). After incubation, cells were collected by cytospin and stained by Hemacolor. GalXM-treated cells exhibited altered morphology, surface blubs and nuclear fragmentation (arrows). N = cell nucleus; original magnification 40x. In selected experiments, cells were incubated for 18 h in the presence or absence (NS) of PHA (10 µg/ml), mAb to CD3 (1 µg/ml) or GalXM (10 µg/ml). After incubation, the percentage of cells undergoing apoptosis was evaluated by PI staining and analyzed using FACScan flow cytometry. Data are expressed as fold increase of percentage of apoptotic cells ( B ), or shown as FACScan histograms from one representative experiment out of seven with similar results ( C ). *, p <0.05 (treated vs untreated, n = 7). Error bars denote s.e.m.

Journal: PLoS ONE

Article Title: Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage

doi: 10.1371/journal.pone.0012720

Figure Lengend Snippet: ( A ) BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were incubated for 18 h in the presence or absence (NS) of PHA (10 µg/ml) or GalXM (10 µg/ml). After incubation, cells were collected by cytospin and stained by Hemacolor. GalXM-treated cells exhibited altered morphology, surface blubs and nuclear fragmentation (arrows). N = cell nucleus; original magnification 40x. In selected experiments, cells were incubated for 18 h in the presence or absence (NS) of PHA (10 µg/ml), mAb to CD3 (1 µg/ml) or GalXM (10 µg/ml). After incubation, the percentage of cells undergoing apoptosis was evaluated by PI staining and analyzed using FACScan flow cytometry. Data are expressed as fold increase of percentage of apoptotic cells ( B ), or shown as FACScan histograms from one representative experiment out of seven with similar results ( C ). *, p <0.05 (treated vs untreated, n = 7). Error bars denote s.e.m.

Article Snippet: CD45 tyrosine phosphatase inhibitor BN82002 hydrochloride salt (BN82002) was obtained from Sigma-Aldrich.

Techniques: Incubation, Staining, Flow Cytometry

BW5147 and BW5147 (T200 − ) cells (both 5×10 6 /ml) were pre-activated for 30 min in the presence or absence (NS) of mAb to CD3 (1 µg/ml) and then incubated for 10 or 30 min in the presence or absence (NS) of GalXM (10 µg/ml). After incubation, cell lysates were analyzed by Western blotting. Membranes were incubated with antibodies to phospho-Lck and Lck. pLck was normalized against Lck. *, p <0.05 (mAb to CD3 plus GalXM treated vs untreated, n = 5). Error bars denote s.e.m.

Journal: PLoS ONE

Article Title: Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage

doi: 10.1371/journal.pone.0012720

Figure Lengend Snippet: BW5147 and BW5147 (T200 − ) cells (both 5×10 6 /ml) were pre-activated for 30 min in the presence or absence (NS) of mAb to CD3 (1 µg/ml) and then incubated for 10 or 30 min in the presence or absence (NS) of GalXM (10 µg/ml). After incubation, cell lysates were analyzed by Western blotting. Membranes were incubated with antibodies to phospho-Lck and Lck. pLck was normalized against Lck. *, p <0.05 (mAb to CD3 plus GalXM treated vs untreated, n = 5). Error bars denote s.e.m.

Article Snippet: CD45 tyrosine phosphatase inhibitor BN82002 hydrochloride salt (BN82002) was obtained from Sigma-Aldrich.

Techniques: Incubation, Western Blot

( A ) BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were pre-activated for 30 min in the presence or absence (NS) of PHA (10 µg/ml) and then incubated for 30 min in the presence or absence (NS) of GalXM (10 µg/ml) or BN82002 (6 µM). After incubation cells were labelled with antibody to phospho-ZAP70 and then analyzed by FACScan flow cytometry. The mean of fluorescence intensity (MFI) of labelled cells is shown as a histogram. *, p <0.05 (treated vs untreated, n = 7). ( B ) BW5147 and BW5147 (T200 − ) cells (both 5×10 6 /ml) were pre-activated for 30 min in the presence or absence (NS) of mAb to CD3 (1 µg/ml) and then incubated for 30 min in the presence or absence (NS) of GalXM (10 µg/ml) or BN82002 (6 µM). After incubation, cell lysates were analyzed by Western blotting; membranes were incubated with antibodies to phospho-Erk1/2 and Erk1/2. pErk was normalized against Erk. *, p <0.05 (treated vs untreated, n = 7). Error bars denote s.e.m. ( C ) BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were pre-activated for 30 min in the presence or absence (NS) of PHA (10 µg/ml) and then incubated for 48 h in the presence or absence (NS) of GalXM (10 µg/ml) or BN82002 (6 µM). After incubation, cell proliferation was evaluated by ViaLight Plus Kit. *, p <0.05 (treated vs untreated, n = 7). Error bars denote s.e.m.

Journal: PLoS ONE

Article Title: Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage

doi: 10.1371/journal.pone.0012720

Figure Lengend Snippet: ( A ) BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were pre-activated for 30 min in the presence or absence (NS) of PHA (10 µg/ml) and then incubated for 30 min in the presence or absence (NS) of GalXM (10 µg/ml) or BN82002 (6 µM). After incubation cells were labelled with antibody to phospho-ZAP70 and then analyzed by FACScan flow cytometry. The mean of fluorescence intensity (MFI) of labelled cells is shown as a histogram. *, p <0.05 (treated vs untreated, n = 7). ( B ) BW5147 and BW5147 (T200 − ) cells (both 5×10 6 /ml) were pre-activated for 30 min in the presence or absence (NS) of mAb to CD3 (1 µg/ml) and then incubated for 30 min in the presence or absence (NS) of GalXM (10 µg/ml) or BN82002 (6 µM). After incubation, cell lysates were analyzed by Western blotting; membranes were incubated with antibodies to phospho-Erk1/2 and Erk1/2. pErk was normalized against Erk. *, p <0.05 (treated vs untreated, n = 7). Error bars denote s.e.m. ( C ) BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were pre-activated for 30 min in the presence or absence (NS) of PHA (10 µg/ml) and then incubated for 48 h in the presence or absence (NS) of GalXM (10 µg/ml) or BN82002 (6 µM). After incubation, cell proliferation was evaluated by ViaLight Plus Kit. *, p <0.05 (treated vs untreated, n = 7). Error bars denote s.e.m.

Article Snippet: CD45 tyrosine phosphatase inhibitor BN82002 hydrochloride salt (BN82002) was obtained from Sigma-Aldrich.

Techniques: Incubation, Flow Cytometry, Fluorescence, Western Blot

( A ) BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were pre-treated for 10 min with ASN 05260638 (20 µM) and then incubated for 18 h in the presence or absence (NS) of GalXM (10 µg/ml). After incubation, the percentage of cells undergoing apoptosis was evaluated by PI staining and analyzed using FACScan flow cytometry. Data are expressed as FACScan histograms from one representative experiment out of five with similar results. ( B ) Schematic representation of GalXM effect on BW5147 cells. GalXM induces immunosuppressive effects on T cells through Lck inactivation. This effects occurs after GalXM association to CD45, which inhibits its dephosphatase activity.

Journal: PLoS ONE

Article Title: Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage

doi: 10.1371/journal.pone.0012720

Figure Lengend Snippet: ( A ) BW5147 and BW5147 (T200 − ) cells (both 1×10 6 /ml) were pre-treated for 10 min with ASN 05260638 (20 µM) and then incubated for 18 h in the presence or absence (NS) of GalXM (10 µg/ml). After incubation, the percentage of cells undergoing apoptosis was evaluated by PI staining and analyzed using FACScan flow cytometry. Data are expressed as FACScan histograms from one representative experiment out of five with similar results. ( B ) Schematic representation of GalXM effect on BW5147 cells. GalXM induces immunosuppressive effects on T cells through Lck inactivation. This effects occurs after GalXM association to CD45, which inhibits its dephosphatase activity.

Article Snippet: CD45 tyrosine phosphatase inhibitor BN82002 hydrochloride salt (BN82002) was obtained from Sigma-Aldrich.

Techniques: Incubation, Staining, Flow Cytometry, Activity Assay

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